Quantitative measures of femoral fracture repair in rats derived by micro-computed tomography

Nyman, Jeffry S.; Munoz, Steve; Jadhav, Satyawan B.; Mansour, Alfred; Yoshii, Toshitaka; Mundy, Gregory R.; Gutiérrez, Gloria

doi:10.1016/j.jbiomech.2009.01.016

Cited by 73 publications

(94 citation statements)

References 25 publications

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“…After scanning and reconstruction, a region of interest (ROI) including the osteotomy site was determined, and quantification of bone morphometry was performed. 15 …”

Section: Specimen Harvestmentioning

confidence: 99%

Endothelial progenitor cells promote fracture healing in a segmental bone defect model

Ateşok

Stewart

et al. 2010

Journal Orthopaedic Research

103

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The objective of this study was to evaluate the effects of local endothelial progenitor cell (EPC) therapy on bone regeneration in a rat model. A segmental bone defect (5 mm) was created in the femur and fixed with a mini-plate. There were two groups: EPC-treated (N ¼ 28) and control (N ¼ 28). Seven animals were sacrificed from each group at 1, 2, 3, and 10 weeks postoperatively. Healing of the defect was evaluated with radiographic, histological, and quantitative micro-computed tomography (micro-CT) scans. Radiographically, mean scores of the EPC and control groups were, respectively, 1.16-0.61 (p < 0.05) at 1 week, 2.53-1.54 (p < 0.05) at 2 weeks, and 4.58-2.35 at 3 weeks (p < 0.05). At 10 weeks, all the animals in the EPC-treated group had complete union (7/7), but in the control group none achieved union (0/7). Histological evaluation revealed that specimens from EPC-treated animals had abundant new bone and vessel formation compared to that in controls. Micro-CT assessment of the samples from the animals sacrificed at 10 weeks (N ¼ 14) showed significantly improved parameters of bone volume (36.58-10.57, p ¼ 0.000), bone volume density (0.26-0.17, p ¼ 0.000), model index À2.22-2.79, p ¼ 0.000), trabecular number (1.28-0.91, p ¼ 0.063), trabecular thickness (0.21-0.15, p ¼ 0.001), trabecular spacing (0.63-1.07, p ¼ 0.022), bone surface (353.75-152.08, p ¼ 0.000), and bone surface to bone volume ratio (9.54-14.24, p ¼ 0.004) for the EPC group compared to control, respectively. In conclusion, local EPC therapy significantly enhanced bone regeneration in a segmental defect model in rat femur diaphysis. ß

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“…After scanning and reconstruction, a region of interest (ROI) including the osteotomy site was determined, and quantification of bone morphometry was performed. 15 …”

Section: Specimen Harvestmentioning

confidence: 99%

Endothelial progenitor cells promote fracture healing in a segmental bone defect model

Ateşok

Stewart

et al. 2010

Journal Orthopaedic Research

103

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“…A custom processing module was then used for stereological analysis (CT analyzer software; SkyScan, Kontich, Belgium). Consistent with the results of other papers that have investigated fracture-healing in a rodent closed femoral fracture model [15][16][17] , stereological parameters-including bone volume, total volume, bone volume fraction, bone mineral density, tissue mineral density, and bone mineral content-were calculated. Minimum and average computed tomography (CT)-based torsional rigidity were also calculated with the method developed by Nazarian and colleagues 18 .…”

Section: Stereological Analysismentioning

confidence: 78%

Parameters for Lithium Treatment Are Critical in Its Enhancement of Fracture-Healing in Rodents

Bernick

Wang

Sigal

et al. 2014

The Journal of Bone and Joint Surgery

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“…Micro-CT imaging provides qualitative and quantitative analyses of bone such as bridging cortices fusion and volumetric BMD (Habermann et al 2010;Nyman et al 2009). As expected from the micro-CT analysis, the worst fracture healing was observed in the ORX group while the best healing was observed in the TEN group.…”

Section: Discussionmentioning

confidence: 99%

Effects of Eurycoma Longifolia on Fracture Healing of Androgen-Deficient Osteoporosis Model: A Micro Computed Tomograph Analysis

Shuid¹

2016

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ABSTRAK Tomografi berkomputer mikro (mikro-CT) adalah peralatan yang berguna untuk memantau pemulihan patah tulang pada model osteoporosis. Ia menyediakan imej 3D kalus fraktur yang boleh digunakan untuk mengira parameter tulang secara kuantitatif. Dalam kajian in, mikro & Health 2016;11(2): 267-277 Azri J. et al. osteoporotic fracture in males was associated with significant morbidity and has huge impact emotionally and financially on their families and the society (Johnell et al. 2001).There are three phases of fracture healing; the reactive phase, reparative phase and remodeling phase. In the reactive phase, platelets are activated to release products such as transforming growth factor-β that triggers inflammation. At the same time, haematoma is formed to seal off the fracture area (Grundnes & Reikeras 1993;Marsh & Li 1999). In the reparative phase, granulation tissue is replaced by fibrocartilage and external callus is formed by intramembranous ossification at the periosteum. Finally, in the remodeling phase, the callus and ABSTRACTMicro computed tomography (micro-CT) imaging is a useful tool to monitor fracture healing in osteoporosis model. It creates a 3-D image of the fracture callus which can be analysed to assess bone parameters quantitatively. In this study, micro-CT was used to assess the fracture healing of orchidectomised rats, an androgen-deficient osteoporosis model. The effects of Eurycoma longifolia, a medicinal plant with proandrogenic effects, on fracture healing were assessed. The rats were grouped into orchidectomised-control (ORX), sham-operated (SHAM), orchidectomised and injected with testosterone intramuscularly once weekly (TEN) and orchidectomised and daily oral gavage of Eurycoma longifolia (EL). Treatment duration was six weeks following bone fracture. Fracture was induced in the right tibia of all the rats. A total of 100 axial slices above and below fracture line were scanned with a micro-CT. The micro-CT analysis was able to detect significant difference in the fracture healing rate of ORX and TEN groups. The bridging cortices and fraction of mineralized tissue of the bridging cortices of the callous of ORX group was significantly lower than TEN group. No significant micro-CT changes were seen in the fracture healing of the EL group. The effect of EL on fracture healing was not demonstrable in orchidectomised rat model.

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Quantitative measures of femoral fracture repair in rats derived by micro-computed tomography

Cited by 73 publications

References 25 publications

Endothelial progenitor cells promote fracture healing in a segmental bone defect model

Endothelial progenitor cells promote fracture healing in a segmental bone defect model

Parameters for Lithium Treatment Are Critical in Its Enhancement of Fracture-Healing in Rodents

Effects of Eurycoma Longifolia on Fracture Healing of Androgen-Deficient Osteoporosis Model: A Micro Computed Tomograph Analysis

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