Quantitative method for measuring adjuvant-induced granuloma angiogenesis in insulin-treated diabetic mice.

Kimura, Masayasu; Amemiya, Kouji; Yamada, Tadashi; Suzuki, Jun

doi:10.1248/bpb1978.9.442

Cited by 44 publications

(31 citation statements)

References 6 publications

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“…This is supported by the fact that insulin treatment restored the impaired inflammatory responses in diabetes. As described in a previous paper (Kimura et al, 1986), in sulin does not enhance granuloma formation in normal mice, but it restores the suppressed formation observed in alloxan diabetic mice. In this study, diabecit KK-CA y mice did not have decreased granuloma formation, but a relative decrease can be detected in the mice having greater body weight when it is considered that ddY mice also show a positive correlationship between body weight and granuloma weight.…”

Section: Discussionsupporting

confidence: 65%

“…Factors affecting angiogenesis are also important since poor angiogenesis occurs prominently in diabetic mice, as previously reported (Kimura et al, 1986).…”

Section: Discussionmentioning

confidence: 59%

“…We have reported the effect of insulin on angiogenesis (Kimura et al, 1986) in adjuvant-induced pouch granuloma (Kimura et al, 1985). In this form of experimental inflammation, poor angiogenesis was observed in alloxan-induced diabetes and this was completely restored by insulin treatment.…”

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confidence: 99%

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Insulin Resistance of Adjuvant-Induced Granuloma Pouch Formation in Genetically Diabetic KK-CA<SUP>y</SUP> Mice

et al. 1987

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The characteristics of adjuvant-induced pouch granuloma in genetically diabetic KK-CAy mice with hyperinsulinemia were investigated. Both the dose-response relationship and the time-course experiments showed that the wet weight of pouch granuloma in diabetic KK-CAy mice was lower than in ddY normal mice. Insulin treatment enhanced granuloma formation in KKCAy mice, and it restored the suppressed DNA content in the granuloma tissue to the level in ddY mice. Although the DNA content was dosedependently increased by insulin, the ratio of DNA content to granuloma weight was constant. In severely diabetic mice, the granuloma weight was not different from that in normoglycemic mice, despite significantly higher blood insulin levels and greater body weight. Insulin stimulated granuloma formation in severely diabetic KK-CAy mice only when higher doses (1mg/kg) were given. This evidence suggests that suppression of granuloma formation in diabetic KK-CAy mice is due to insulin resistance and that restoration requires pharmacological doses of insulin.

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Section: Discussionsupporting

confidence: 65%

“…Factors affecting angiogenesis are also important since poor angiogenesis occurs prominently in diabetic mice, as previously reported (Kimura et al, 1986).…”

Section: Discussionmentioning

confidence: 59%

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Insulin Resistance of Adjuvant-Induced Granuloma Pouch Formation in Genetically Diabetic KK-CA<SUP>y</SUP> Mice

et al. 1987

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“…The volume fraction occupied by the blood vessels was estimated using a 486-point square lattice (18 Â 27) with a field-ofview of 0.209 mm 2 . Functional vessels were determined in a similar manner following intravenous injection of carmine red dye (Sigma) (Kimura et al, 1986). In these experiments, 1 ml of a saline solution containing 10% (w v À1 ) carmine red dye and 5% (w v À1 ) gelatin were injected into the tail veins of anaesthetised animals.…”

Section: Tumour Histology and Immunohistochemical Examinationmentioning

confidence: 99%

Early detection of tumour immune-rejection using magnetic resonance imaging

et al. 2003

Br J Cancer

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Dynamic contrast agent-enhanced magnetic resonance imaging measurements of the perfusion of an immunogenic murine tumour showed that immune rejection was preceded by an increase in the apparent vascular volume of the tumour. This increase in vascularity, which has been observed previously in other tumours undergoing immune rejection, was confirmed by histological analysis of tumour sections obtained postmortem. Magnetic resonance imaging measurements similar to this could be used in the clinic to monitor the early responses of tumours to immunotherapy, before there is any change in tumour growth rate or volume.

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“…Measurement and visualization of the angiogenesis in the granulation tissue were carried out using carmine dye (Natural Red 4, Sigma Chemical Co.) according to the method described by Kimura et al (1986) and Colville-Nash et al (1995) with slight modi®cations (Ghosh et al, 2000). Six days after injection of the carrageenin solution, 3 ml of a 5% (w v 71 ) solution of carmine dye in saline containing 5% (w v 71 ) gelatin (Sankoh Jun-yaku, Tokyo, Japan) at 378C was injected into the tail vein of each rat.…”

Section: Determination Of Angiogenesis In Carrageenin-induced Granulamentioning

confidence: 99%

Inhibition by acharan sulphate of angiogenesis in experimental inflammation models

Ghosh

Hirasawa

Lee

et al. 2002

British J Pharmacology

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1 The e ects of acharan sulphate, a glycosaminoglycan isolated from the giant African snail Achatina fulica, on angiogenesis in the granulation tissue were analysed using an air pouch-type carrageenin-induced in¯ammation model in rats and a cotton thread-induced in¯ammation model in mice. 2 In the carrageenin-induced in¯ammation model in rats, intra-pouch injections of acharan sulphate (5 and 50 mg) inhibited the pouch¯uid accumulation and the granulation tissue formation as well as the angiogenesis in the granulation tissue at day 6 in a dose-dependent manner. 3 The inhibitory e ects of acharan sulphate at 50 mg on the pouch¯uid accumulation and the leucocyte in®ltration into the pouch¯uid was not so e ective as that of the cyclo-oxygenase inhibitor indomethacin at 100 mg, but the inhibitory e ects of acharan sulphate at 50 mg on the granulation tissue formation and angiogenesis in the granulation tissue were almost the same as those of indomethacin at 100 mg. 4 Acharan sulphate did not a ect levels of vascular endothelial growth factor (VEGF) in the granulation tissue and in the pouch¯uid at day 6, but indomethacin signi®cantly lowered them. 5 In the cotton thread-induced in¯ammation model in mice, injections of acharan sulphate (10 mg) at the site of the cotton thread implantation inhibited the granulation tissue formation and angiogenesis as indomethacin (20 mg) did. Acharan sulphate (10 mg) did not a ect levels of VEGF in the cotton thread-induced granulation tissue at day 5, but indomethacin (20 mg) signi®cantly lowered them. 6 In culture of human vascular endothelial cells, acharan sulphate at 10 and 100 mg ml 71 inhibited VEGF-induced capillary tube formation. 7 These ®ndings suggest that the inhibitory e ect of acharan sulphate on angiogenesis in carrageenin-and cotton thread-induced granulation tissues is not due to the inhibition of VEGF protein induction, but is due to the inhibition of VEGF-induced vascular tube formation.

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Quantitative method for measuring adjuvant-induced granuloma angiogenesis in insulin-treated diabetic mice.

Cited by 44 publications

References 6 publications

Insulin Resistance of Adjuvant-Induced Granuloma Pouch Formation in Genetically Diabetic KK-CA<SUP>y</SUP> Mice

Insulin Resistance of Adjuvant-Induced Granuloma Pouch Formation in Genetically Diabetic KK-CA<SUP>y</SUP> Mice

Early detection of tumour immune-rejection using magnetic resonance imaging

Inhibition by acharan sulphate of angiogenesis in experimental inflammation models

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