Quantitative microbial risk assessment of bioaerosols from a manure application site

Jahne, Michael A.; Rogers, Shane; Holsen, Thomas M.; Grimberg, Stefan J.

doi:10.1007/s10453-014-9348-0

Cited by 53 publications

(42 citation statements)

References 67 publications

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“…Additional background samples (n = 6) were collected at nearby fields to which manure had not been applied during the previous 6 mo. Bioaerosol samplers (one each per event) included liquid impingers for the determination of the total ambient concentration by quantitative real-time polymerase chain reaction (qPCR), six-stage impactors for the determination of the size distribution, and static-water surface samplers (SWSS) for the determination of dry deposition flux as described by Jahne et al (2015). Briefly, autoclaved (121°C, 20 psi, for 15 min) liquid impingers (SKC Biosamplers, Eighty Four, PA) were loaded with 20 mL autoclaved phosphate buffered saline (PBS; pH 7.4) and operated at a flow rate of 12.5 L min −1 .…”

Section: Sample Collection and Analysismentioning

confidence: 99%

“…Generic E. coli (Chern et al, 2011) was analyzed in manure samples but not bioaerosol samples owing to the comparatively low manure concentrations. The qPCR reactions were completed in duplicate on a Roche LightCycler 480 as previously described ( Jahne et al, 2015), using cycling parameters reported by the respective authors. The qPCR calibration curves and performance characteristics, including analytical limits of detection, are included in Supplemental Table S4; sources of DNA standards are provided in Supplemental Table S5.…”

Section: Sample Collection and Analysismentioning

confidence: 99%

“…Bioaerosol emission and transport for each sampling event were modeled with the AERMOD dispersion model using Lakes Environmental AERMOD View as described by Jahne et al (2015). Data sources included ArcGIS (ESRI) base maps and field shapefiles, surface and upper air meteorological data preprocessed from the Fifth-Generation Penn State/National Center for Atmospheric Research Mesoscale Model (Lakes Environmental), USGS surface and terrain data (National Land Cover Data and 15-min digital elevation model), bioaerosol size distribution measured by six-stage impactors, and onsite meteorology (1-min average temperature, relative humidity, wind speed, and wind direction) logged by a Pegasus EX weather station (Columbia Weather Systems).…”

Section: Emission and Transport Modelingmentioning

confidence: 99%

“…A pplication of livestock manure to agricultural land is a valuable farming technique, with twofold benefits of waste management and fertilization. However, zoonotic pathogens in applied manure can be released from application sites through surface runoff and aerosol transport ( Jahne et al, 2015;USEPA, 2005). In addition to direct human exposure, these pathogens may present public health risks through contamination of food crops; an estimated two-thirds of deaths from food-borne disease are due to zoonotic bacterial pathogens also found in animal manure (Bowman, 2009;USEPA, 2013).…”

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confidence: 99%

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Bioaerosol Deposition to Food Crops near Manure Application: Quantitative Microbial Risk Assessment

et al. 2016

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Production of both livestock and food crops are central priorities of agriculture; however, food safety concerns arise where these practices intersect. In this study, we investigated the public health risks associated with potential bioaerosol deposition to crops grown in the vicinity of manure application sites. A field sampling campaign at dairy manure application sites supported the emission, transport, and deposition modeling of bioaerosols emitted from these lands following application activities. Results were coupled with a quantitative microbial risk assessment model to estimate the infection risk due to consumption of leafy green vegetable crops grown at various distances downwind from the application area. Inactivation of pathogens ( spp., spp., and O157:H7) on both the manure-amended field and on crops was considered to determine the maximum loading of pathogens to plants with time following application. Overall median one-time infection risks at the time of maximum loading decreased from 1:1300 at 0 m directly downwind from the field to 1:6700 at 100 m and 1:92,000 at 1000 m; peak risks (95th percentiles) were considerably greater (1:18, 1:89, and 1:1200, respectively). Median risk was below 1:10,000 at >160 m downwind. As such, it is recommended that a 160-m setback distance is provided between manure application and nearby leafy green crop production. Additional distance or delay before harvest will provide further protection of public health.

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Section: Sample Collection and Analysismentioning

confidence: 99%

Section: Sample Collection and Analysismentioning

confidence: 99%

Section: Emission and Transport Modelingmentioning

confidence: 99%

mentioning

confidence: 99%

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Bioaerosol Deposition to Food Crops near Manure Application: Quantitative Microbial Risk Assessment

et al. 2016

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“…This is mostly due to high manure production and public acceptance of manure application practice for thousands of years [62]. In addition, most of the studies focus on the pathogens in biosolids, and there is lack of comparison studies for pathogens in manure [20,62].…”

Section: Characterization Of Biosolids and Manurementioning

confidence: 99%

Effects of Biosolids and Manure Application on Microbial Water Quality in Rural Areas in the US

Oun

Kumar

Harrigan

et al. 2014

Water

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Most of the waterborne disease outbreaks observed in North America are associated with rural drinking water systems. The majority of the reported waterborne outbreaks are related to microbial agents (parasites, bacteria and viruses). Rural areas are characterized by high livestock density and lack of advanced treatment systems for animal and human waste, and wastewater. Animal waste from livestock production facilities is often applied to land without prior treatment. Biosolids (treated municipal wastewater sludge) from large wastewater facilities in urban areas are often transported and applied to land in rural areas. This situation introduces a potential for risk of human exposure to waterborne contaminants such as human and zoonotic pathogens originating from manure, biosolids, and leaking septic systems. This paper focuses on waterborne outbreaks and sources of microbial pollution in rural areas in the US, characterization of the microbial load of biosolids and manure, association of biosolid and manure application with microbial contamination of surface and groundwater, risk assessment and best management practice for biosolids and manure application to protect water quality. Gaps in knowledge are identified, and recommendations to improve the water quality in the rural areas are discussed. OPEN ACCESS

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Bioaerosol Sources

Wéry

Galès

2017

Microbiology of Aerosols

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Quantitative microbial risk assessment of bioaerosols from a manure application site

Cited by 53 publications

References 67 publications

Bioaerosol Deposition to Food Crops near Manure Application: Quantitative Microbial Risk Assessment

Bioaerosol Deposition to Food Crops near Manure Application: Quantitative Microbial Risk Assessment

Effects of Biosolids and Manure Application on Microbial Water Quality in Rural Areas in the US

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