Quantitative morphometric patterns in cartilage and bone from the humeral heads of end-stage osteoarthritis patients

Pawson, D.J.; Glanzmann, Michael C.; Luechinger, Roger; Müller, Ralph; Stok, Kathryn S.

doi:10.1016/j.joca.2015.04.009

Cited by 15 publications

(20 citation statements)

References 39 publications

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“…Although, subchondral osteoclasts were previously considered by many investigators to be the key regulatory cell type in subchondral‐bone contributing to OA progress (Babaji et al, ; Bertuglia et al, ; Dong et al, ), recent studies have suggested different pathological progression in OA, as PTOA exhibited subchondral bone sclerosis rather than subchondral bone loss (Osterberg et al, ; Pawson, Glanzmann, Luechinger, Muller, & Stok, ). Our structural data indicated that, while the total subchondral bone TV and Tb.Sp declined, the BV/TV and Tb.Th increased (Figure d–g), suggesting presence of subchondral bone sclerosis.…”

Section: Discussionmentioning

confidence: 99%

Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post‐traumatic osteoarthritis

Lin

Shao

Zeng

et al. 2018

Journal Cellular Physiology

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PI3K/AKT signaling is essential in regulating pathophysiology of osteoarthritis (OA). However, its potential modulatory role in early OA progression has not been investigated yet. Here, a mouse destabilization OA model in the tibia was used to investigate roles of PI3K/AKT signaling in the early subchondral bone changes and OA pathological process. We revealed a significant increase in PI3K/AKT signaling activation which was associated with aberrant bone formation in tibial subchondral bone following destabilizing the medial meniscus (DMM), which was effectively prevented by treatment with PI3K/AKT signaling inhibitor LY294002. PI3K/AKT signaling inhibition attenuated articular cartilage degeneration. Serum and bone biochemical analyses revealed increased levels of MMP-13, which was found expressed mainly by osteoblastic cells in subchondral bone. However, this MMP-13 induction was attenuated by LY294002 treatment. Furthermore, PI3K/AKT signaling was found to enhance preosteoblast proliferation, differentiation, and expression of MMP-13 by activating NF-κB pathway. In conclusion, inhibition of PI3K/AKT/NF-κB axis was able to prevent aberrant bone formation and attenuate cartilage degeneration in OA mice.

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Section: Discussionmentioning

confidence: 99%

Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post‐traumatic osteoarthritis

Lin

Shao

Zeng

et al. 2018

Journal Cellular Physiology

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“…Third, for this study, we used soft cushions filled with Fomblin oil. 18 It is not approved as a medical product and cannot be used in clinical routine. According to our personal experience, Fomblin oil is well suited to reduce susceptibility effects at tissue-air interfaces.…”

Section: Discussionmentioning

confidence: 99%

“…To reduce susceptibility effects at tissue-air interfaces, soft cushions filled with Fomblin oil (200 mL and 400 mL; Solvay, Italy) were placed on top and around the patella at both field strengths. 18 Legs were immobilized using sand bags and straps. Possible adverse effects of 3 T and 7 T imaging with respect to duration of the symptom, intensity, requirement for further treatment, and relationship to medical device were recorded.…”

Section: Study Subjectsmentioning

confidence: 99%

Clinical Magnetic Resonance Imaging of the Knee at 7 T

et al. 2019

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The aim of this study was to evaluate the efficiency and performance of different fat suppression techniques for clinical 7 T knee magnetic resonance imaging including the slice-selective gradient reversal (SSGR) technique. MATERIALS AND METHODS This article is an ethical boardapproved prospective study with written informed consent from 8 volunteers (mean, 31 ± 4 years). It included fat phantom and knee magnetic resonance imaging at 3 T (Magnetom Skyra; Siemens Healthcare) and at 7 T (Achieva, Philips Healthcare). At 3 T, an axial proton density-weighted turbo spin echo sequence with spectral adiabatic inversion recovery (SPAIR) was acquired. At 7 T, a series of 5 proton density-weighted turbo spin echo sequences was acquired: (a) without fat suppression, (b) with spectral presaturation with inversion recovery (SPIR), (c) with SPAIR, (d) with SSGR, and (e) with the combination of SSGR + SPIR. Additional noise scans allowed pixelwise calculation of signal-to-noise ratio and contrast-to-noise ratio maps. Quantitative data at 7 T were compared with each other but not to 3 T. Two independent radiologists evaluated overall image quality, homogeneity and grade of fat suppression, and the delineation between 2 adjacent structures. Results were compared using Wilcoxon signed rank and paired sample t tests. RESULTS Relative signal-to-noise ratios of fat demonstrated that the SPIR technique reduced the fat signal to 45% ± 5.4%; SPAIR, 18% ± 1.2%; SSGR, 14% ± 1.1%; and SSGR + SPIR, 11% ± 0.3%. Contrast-to-noise ratio showed superior contrast between muscle-fat (P < 0.001) and fluid-fat (P 0.001) for SSGR and SSGR + SPIR. The radiologists rated the overall image quality higher at 7 T than 3 T. The homogeneity and grade of fat suppression as well as the delineation between 2 different (adjacent) structures were rated best for SSGR + SPIR. CONCLUSIONS At 7 T, fat saturation for clinical knee imaging using SSGR and the combination of SSGR + SPIR was superior compared with standard methods based on spectrally selective radiofrequency pulses.

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“…Numerous studies were based on morphometric analyses of relevance to bones specimens (osteology-based) [1][2][3][4][5]. The morphometric studies of bones were usually focusing on observing and quantifying; morphologies and shapes, dimensional parameters, surface area of articular and non-articular regions, and volumetric measurements (volumetry) of a whole bone or segments of bone (processes, tubercles, tuberosities, cavities, sinuses, and others) [2,[5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A Biomedical Device Innovation: A Method for Accurate Calculation of Volume (Volumetry) of Irregular Bone Specimens

Imam¹

2017

MOJAP

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The morphometric studies are considered to be abundant in connection with the study of Human Anatomy and Comparative Anatomy. The estimation of volume, also known as volumetry, is integral to these studies although it is not frequently included. Several methods exist to measure the accurate size (volume) of bones and certain soft tissue; these techniques are not limited to replica manufacturing and the implementation of Archimedes' principles of buoyancy and fluid displacement. The latter will be explored in connection with a prospective biomedical innovation of a device for the purpose of estimation of the size of bones particularly those of variable shapes, dimensions, and highly irregular shapes.

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Quantitative morphometric patterns in cartilage and bone from the humeral heads of end-stage osteoarthritis patients

Abstract: The ability to correlate bone and cartilage changes is valuable, as these structural cues may allow a more targeted diagnostic approach and a better classification of the disease, leading to improved therapies.

Cited by 15 publications

References 39 publications

Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post‐traumatic osteoarthritis

Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post‐traumatic osteoarthritis

Clinical Magnetic Resonance Imaging of the Knee at 7 T

A Biomedical Device Innovation: A Method for Accurate Calculation of Volume (Volumetry) of Irregular Bone Specimens

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