Quantitative Multiplex Detection of Pathogen Biomarkers on Multichannel Waveguides

Mukundan, Harshini; Xie, Hongtu; Price, Dominique N.; Kubicek-Sutherland, Jessica Z.; Grace, W. Kevin; Anderson, Aaron S.; Martinez, Jennifer S.; Hartman, N. F.; Swanson, Basil I.

doi:10.1021/ac901497g

Cited by 46 publications

(52 citation statements)

References 15 publications

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“…Lipid micelles for use in waveguide experiments were prepared as described previously [2,5,13]. Briefly, 2 mM DOPC and 1% cap-biotinyl (mol/mol) were combined in a glass tube and the chloroform evaporated under argon gas.…”

Section: Lipid Micelle Preparationmentioning

confidence: 99%

“…Silicon oxynitride waveguides were purchased from nGimat (Norcross, GA), functional silicon dioxide surfaces were maintained by Spectrum Thin Films (Hauppauge, NY) and waveguides were cleaned/ prepared as described previously [2,3,8,13]. Briefly, waveguides were cleaned by sonication in a water bath for 5 min each in chloroform, ethanol, and then nanopure water.…”

Section: Waveguide Preparation and Flow Cell Assemblymentioning

confidence: 99%

“…Immunoassays were performed on a waveguide-based optical biosensor [2,3,8,13]. All incubations occurred at room temperature.…”

Section: Lta Membrane Insertion Assaymentioning

confidence: 99%

“…The sensor detects fluorescently labeled antibodies that probe only surface-bound molecules, within the evanescent field, resulting in minimal autofluorescent noise in complex samples (such as serum). This platform also allows for a multiplex format in which a suite of biomarkers can be detected simultaneously using multichannel waveguides and photostable and tunable quantum dots as the fluorescence reporter [2]. Measurements are extremely rapid (3 sec), yield minimal non-specific and background signals, and can be adapted to the simultaneous detection of a wide variety of fluorescent labels.…”

Section: Introductionmentioning

confidence: 99%

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Membrane Insertion for Direct Detection of Lipoteichoic Acid

Kubicek-Sutherland

Hengartner

Mukundan

2017

Trans. Mat. Res. Soc. Japan

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The incorrect diagnosis of bacterial pathogens results in the misuse of antibiotics, a major contributor to the evolution of antibiotic resistance. The ability to rapidly identify a pathogen could facilitate more effective treatment strategies, reducing the unnecessary use of antibiotics. Current diagnostics of bacterial infections often require cell growth, and are therefore less capable of informing timely treatment options. Here, we describe the development of a rapid assay (<45 min) to identify Gram-positive bacteria directly in human serum without any growth or isolation required. We have developed a fluorescence-based immunoassay specifically targeting the Gram-positive bacterial biomarker lipoteichoic acid (LTA). LTA is an amphiphilic molecule with both hydrophobic and hydrophilic portions. Our approach, termed membrane insertion, exploits the amphiphilic biochemistry of the molecule by allowing it to passively insert itself into a lipid bilayer, thereby trapping it for subsequent probing with a fluorescently labeled α-LTA antibody. Using this assay, we have successfully detected less than 10 µg/ml of LTA directly in spiked human serum. We intend to combine this rapid assay with others currently in development into a diagnostic tool for point-of-care detection of bacterial pathogens.

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Section: Lipid Micelle Preparationmentioning

confidence: 99%

Section: Waveguide Preparation and Flow Cell Assemblymentioning

confidence: 99%

“…Immunoassays were performed on a waveguide-based optical biosensor [2,3,8,13]. All incubations occurred at room temperature.…”

Section: Lta Membrane Insertion Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Membrane Insertion for Direct Detection of Lipoteichoic Acid

Kubicek-Sutherland

Hengartner

Mukundan

2017

Trans. Mat. Res. Soc. Japan

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“…Recently, QDs have been used in a dual-parameter assay performed in a multichannel waveguide-based chip. The assay can be completed in 15 min [60]. Also, multiplexed testing of cancer biomarkers carcinoembryonic antigen, cancer antigen 125, and Her-2/Neu has been done in a microfluidic nanochip using microparticles as solid phase.…”

Section: Fluorescent Labelsmentioning

confidence: 99%

Simple and inexpensive immunoassay-based diagnostic tests

Päkkilä

Soukka

2011

Bioanal Rev

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Simple and inexpensive yet sensitive and robust diagnostic tests are critically needed for resource-poor settings to enable timely diagnosis and effective use of limited health care resources. Current tests are often too expensive, too slow, or have compromised clinical performance, and they often require health care professional to perform the test. In addition, most assays are not intended to be used in extreme environmental conditions, but their performance may be affected by high temperatures and humidity often encountered in resource-poor settings. This review provides an overview of current immunoassay technologies and their advantages and limitations with respect to their feasibility to resourcepoor settings. Future trends of immunoassay development for decentralized testing are also discussed. Homogeneous assays as such are out of the scope of this article because they are generally not yet sensitive enough or otherwise less feasible for inexpensive rapid diagnostic tests.

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Recent progress and challenges on the bioassay of pathogenic bacteria

2020

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The present review (containing 242 references) illustrates the importance and application of optical and electrochemical methods as well as their performance improvement using various methods for the detection of pathogenic bacteria. The application of advanced nanomaterials including hyper branched nanopolymers, carbon‐based materials and silver, gold and so on. nanoparticles for biosensing of pathogenic bacteria was also investigated. In addition, a summary of the applications of nanoparticle‐based electrochemical biosensors for the identification of pathogenic bacteria has been provided and their advantages, detriments and future development capabilities was argued. Therefore, the main focus in the present review is to investigate the role of nanomaterials in the development of biosensors for the detection of pathogenic bacteria. In addition, type of nanoparticles, analytes, methods of detection and injection, sensitivity, matrix and method of tagging are also argued in detail. As a result, we have collected electrochemical and optical biosensors designed to detect pathogenic bacteria, and argued outstanding features, research opportunities, potential and prospects for their development, according to recently published research articles.

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Quantitative Multiplex Detection of Pathogen Biomarkers on Multichannel Waveguides

Cited by 46 publications

References 15 publications

Membrane Insertion for Direct Detection of Lipoteichoic Acid

Membrane Insertion for Direct Detection of Lipoteichoic Acid

Simple and inexpensive immunoassay-based diagnostic tests

Recent progress and challenges on the bioassay of pathogenic bacteria

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