“…It must be emphasised, however, that this worker studied, not growing cultures, but washed suspensions of bacteria. Apart from the difference between measuring manometrically the acid liberated over a short period of time from a heavy suspension of washed cells, and observing the glucose breakdown in an actively growing culture over a period of 24 or 48 hours, there is the further observation, made by Kendall, Friedemann and Ishikawa [1930], that it is difficult to obtain re,producible observations on the rate of chemical changes produced by washed suspensions of bacteria owing to the variable damage caused to the organisms by the washing and centrifuging processes. Finkle's results are not therefore strictly comparable with those described in this communication.…”