Quantitative Occurrence of Antibiotic Resistance Genes among Bacterial Populations from Wastewater Treatment Plants Using Activated Sludge

Osińska, Adriana; Korzeniewska, Ewa; Harnisz, Monika; Niestępski, Sebastian

doi:10.3390/app9030387

Cited by 46 publications

(16 citation statements)

References 40 publications

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“…However, the A/O biological treatment system adopted by swine farm 2 is unfavorable for ARGs removal in piggery wastewater. As the inoculation of activated sludge during A/O biological treatment can increase the abundance and diversity of bacteria in wastewater, which facilitate the proliferation and dissemination of ARGs in piggery wastewater 67 – 69 . But troublesomely, through the treatment system of swine liquid manure, considerable levels of these ARGs were still detected in the final effluent from both farms (10 2 –10 6 copies/mL for PWWTS1; 10 3 –10 8 copies/mL for PWWTS2).…”

Section: Resultsmentioning

confidence: 99%

Swine liquid manure: a hotspot of mobile genetic elements and antibiotic resistance genes

Yang

Han

et al. 2020

Sci Rep

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The overuse or abuse of antibiotics as veterinary medicine and growth promoters accelerates antibiotic resistance, creating a serious threat to public health in the world. Swine liquid manure as an important reservoir of antibiotic resistance genes (ARGs) has received much attention, but little information is known regarding the occurrence, persistence and fate of ARGs-associated mobile genetic elements (MGEs) in swine farms, especially their change patterns and removal in full-scale piggery wastewater treatment systems (PWWTSs). In this study, we searched the presence and distribution of MGEs and associated ARGs in swine farms, and addressed their fate and seasonal variation in full-scale PWWTSs by real-time quantitative PCR (qPCR). Our results revealed class 1 integrons, class 2 integrons and conjugative plasmids were prevalent in pig feces and piggery wastewater. A clear pattern of these MGE levels in swine liquid manure was also observed, i.e., intI1 > intI2 > traA (p < 0.01), and their absolute abundances in winter were all higher than that in summer with 0.07–2.23 logs. Notably, MGEs and ARGs prevailed through various treatment units of PWWTSs, and considerable levels of them were present in the treated effluent discharged from swine farms (up to 101–107 copies/mL for MGEs and 103–108 copies/mL for ARGs). There were significant correlations between most ARG abundance and MGE levels (p < 0.05), such as tetQ and traA (r = 0.775), sul1 and intI1 (r = 0.847), qnrS and inI2 (r = 0.859), suggesting the potential of ARGs—horizontal transfer. Thus the high prevalence and enrichment of MGEs and ARGs occurred in pig feces and piggery wastewater, also implicating that swine liquid manure could be a hotspot for horizontal transfer of ARGs.

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Section: Resultsmentioning

confidence: 99%

Swine liquid manure: a hotspot of mobile genetic elements and antibiotic resistance genes

Yang

Han

et al. 2020

Sci Rep

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“…BOD to COD ratio is an important aggregate measure of wastewater characteristics, indicating the biodegradability of wastewater [ 20 ] and microbial community [ 21 , 22 ]. Typical values for the ratio of BOD/COD for untreated domestic wastewater are in the range from 0.3 to 0.8 [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Antibiotic Resistance Profile of Bacteria Isolated from Wastewater Systems in Eastern Ethiopia

Teshome

Alemayehu

Deriba

et al. 2020

Journal of Environmental and Public Health

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World Health Organizations launched a global action plan on antimicrobial resistance since 2015. Along with other objectives, the plan was aimed to strengthen knowledge of the spread of antimicrobial resistance through surveillance and research. Given their high bacterial densities and that they receive antibiotics, metals, and other selective agents, wastewater systems are a logical hotspot for antibiotic resistance surveillance. The current study reports on the result of antibiotic resistance surveillance conducted in selected wastewater systems of Eastern Ethiopia from Feb. 2018 to Oct. 2019. We monitored three wastewater systems in Eastern Ethiopia, such as the activated sludge system of Dire Dawa University, waste stabilization pond of Haramaya University, and a septic tank of Hiwot Fana Specialized University Hospital for 18 months period. We collected 66 wastewater samples from 11 sampling locations and isolated 722 bacteria using selective culture media and biochemical tests. We tested their antibiotic susceptibility using the standard Kirby-Bauer disk diffusion method on the surface of the Mueller-Hinton agar and interpreted the result according to EUCAST guidelines. The result shows the highest percentage of resistance for ampicillin among isolates of hospital wastewater effluent which is 36 (94.7%), 33 (91.7%), and 32 (88.9%) for E. coli, E. faecalis, and E. faecium, respectively. A lower rate of resistance was seen for gentamicin among isolates of activated sludge wastewater treatment system which is 10 (16.4%), 8 (13.3%), 11 (18.9%), and 12 (20.3%) for E. coli, E. faecalis, E. faecium, and P. aeruginosa, respectively. Hospital wastewater exhibited higher resistance than the other two wastewater systems. The Multiple Antibiotic Resistance Index (MARI) has significantly increased in the wastewater’s course treatment process, showing the proliferation of resistance in the wastewater treatment system.

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“…The development of antimicrobial resistance genes (ARGs) in Enterobacteriaceae members has become a global health problem because antibiotic resistance (AR) leads to limitations in the treatment options thereby increasing the rate of morbidity and mortality [38]. Although carbapenem resistance (CR) among E. coli strains has been globally reported [39,40,41], there is a dearth of information on Carbapenem-resistant Escherichia coli (CREC) isolated from different environmental niches in South Africa. In the Eastern Cape Province, this is the rst study that evaluates the genetic diversity among CREC strains using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) genotyping.…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization Among Carbapenem-resistant Escherichia coli Strains Using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) Fingerprinting in South Africa

Ebomah¹,

Okoh²

2020

Preprint

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Background Carbapenems belong to beta-lactam class of antibiotics usually considered as the last line of defense because they can be effective against severe infections caused by prevalent multidrug-resistant (MDR) pathogens. However, carbapenems can be deactivated by bacteria that produce carbapenemase (beta-lactamase). This study was conducted to screen for carbapenem-resistance genes (CRGs) harbored by pathogenic strains of Escherichia coli recovered from different environmental samples. We also assessed the genetic relatedness among selected E. coli pathotypes using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR).Method: Molecular identification and characterization of the presumptive isolates were performed using PCR and isolates that exhibited antimicrobial resistance (AMR) phenotypically were further screened for some relevant CRGs (blaNDM−1, blaKPC and blaOXA−48−like). Furthermore, ERIC-PCR was used to determine the similarity and diversity of 31 E. coli strains which were randomly selected from the different sources analyzed in this study.Result Our findings revealed a total of 238 presumptive E. coli isolates, out of which 192 were confirmed positive for uidA gene. Further screening revealed 77 (40%) isolates belong to six key E. coli pathotypes and 70 of them exhibited phenotypic AMR. Additionally, twenty-nine (41%) of the 70 MDR pathogenic E. coli strains harbored CRGs; with 24 strains harboring blaNDM−1, 8 harboring blaKPC and 2 harboring blaOXA−48−like genes.Conclusion Findings also suggest that the selected E. coli pathotypes belonged to different genomic clusters, while the cluster analysis showed a possible genetic diversity among aquatic and farm isolates. Proper treatment of final effluents before discharge as well as the development of more effective strategies to control and manage the use of antimicrobial agents were strongly recommended.

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Quantitative Occurrence of Antibiotic Resistance Genes among Bacterial Populations from Wastewater Treatment Plants Using Activated Sludge

Cited by 46 publications

References 40 publications

Swine liquid manure: a hotspot of mobile genetic elements and antibiotic resistance genes

Swine liquid manure: a hotspot of mobile genetic elements and antibiotic resistance genes

Antibiotic Resistance Profile of Bacteria Isolated from Wastewater Systems in Eastern Ethiopia

Genetic Characterization Among Carbapenem-resistant Escherichia coli Strains Using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) Fingerprinting in South Africa

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