Quantitative PCR assay for detection and enumeration of ciguatera-causing dinoflagellate Gambierdiscus spp. (Gonyaulacales) in coastal areas of Japan

Species‐specific PCR assays for Gambierdiscus excentricus and Gambierdiscus silvae (Gonyaulacales, Dinophyceae)

“…Others have subsequently developed equivalent assays for species occurring in the Pacific (Nishimura et al. , Darius et al. , Smith et al.…”

“…To facilitate accurate species identifications, Vandersea et al (2012) developed species-specific quantitative polymerase chain reaction (PCR) assays for identifying many of the Gambierdiscus and Fukuyoa species in the Caribbean. Others have subsequently developed equivalent assays for species occurring in the Pacific (Nishimura et al 2016, Darius et al 2017, Smith et al 2017. Currently, there are no species-specific PCR assays for Gambierdiscus excentricus (Fraga et al 2011) or G. silvae (Fraga and Rodr ıguez 2014).…”

Development of a quantitative PCR assay for the detection and enumeration of a potentially ciguatoxin-producing dinoflagellate, Gambierdiscus lapillus (Gonyaulacales, Dinophyceae)

“…TaqMan Probes & SYBR Green [40,41] G. belizeanus SYBR Green [42] G. caribaeus SYBR Green [42] G. carolinianus SYBR Green [42] G. carpenteri SYBR Green [42] G. pacificus SYBR Green [41] G. polynesiensis SYBR Green [41] G. scabrosus TaqMan Probes [40] G. toxicus SYBR Green [41] Gambierdiscus sp. ribotype 2 SYBR Green [42] Gambierdiscus sp.…”

“…ribotype 2 SYBR Green [42] Gambierdiscus sp. type 2 TaqMan Probes [40] Gambierdiscus sp. type 3 TaqMan Probes [40] Fukuyoa spp.…”

Harmful Algal Blooms in Benthic Systems: Recent Progress and Future Research

“…So far, real-time PCR (qPCR) assays exist for most of the Gambierdiscus species Nishimura et al, 2016) and for O. cf. ovata (Perini et al 2011;Casabianca et al, 2013).…”