Quantitative PCR estimates Angiostrongylus cantonensis (rat lungworm) infection levels in semi-slugs (Parmarion martensi)

Jarvi, Susan I.; Farias, Margaret; Howe, Kay; Jacquier, Steven; Hollingsworth, Robert G.; Pitt, William C.

doi:10.1016/j.molbiopara.2012.08.002

Cited by 39 publications

(65 citation statements)

References 9 publications

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“…30,31 Not unexpectedly, the PCR-positive CSF samples in this study had Ct values between 27 and 38, suggesting that these samples contained 1-4 log 10 less DNA than that present in a specimen containing a single larva. This indicates that the real-time PCR can detect DNA from remnants of larvae, such as individual cells, nuclei, or chromosomal fragments, which may leak into the CSF from the brain.…”

Section: Discussionmentioning

confidence: 70%

Real-Time Polymerase Chain Reaction Detection of Angiostrongylus cantonensis DNA in Cerebrospinal Fluid from Patients with Eosinophilic Meningitis

Qvarnström¹,

Xayavong²,

Silva³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Angiostrongylus cantonensis is the most common infectious cause of eosinophilic meningitis. Timely diagnosis of these infections is difficult, partly because reliable laboratory diagnostic methods are unavailable. The aim of this study was to evaluate the usefulness of a real-time polymerase chain reaction (PCR) assay for the detection of A. cantonensis DNA in human cerebrospinal fluid (CSF) specimens. A total of 49 CSF specimens from 33 patients with eosinophilic meningitis were included: A. cantonensis DNA was detected in 32 CSF specimens, from 22 patients. Four patients had intermittently positive and negative real-time PCR results on subsequent samples, indicating that the level of A. cantonensis DNA present in CSF may fluctuate during the course of the illness. Immunodiagnosis and/or supplemental PCR testing supported the real-time PCR findings for 30 patients. On the basis of these observations, this real-time PCR assay can be useful to detect A. cantonensis in the CSF from patients with eosinophilic meningitis.

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Section: Discussionmentioning

confidence: 70%

Real-Time Polymerase Chain Reaction Detection of Angiostrongylus cantonensis DNA in Cerebrospinal Fluid from Patients with Eosinophilic Meningitis

Qvarnström¹,

Xayavong²,

Silva³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…This nematode has been isolated in giant African land snails elsewhere (Kim et al, 2014;Iwanowicz et al, 2015). Angiostrongylus cantonensis is a common parasite of rats and the cause of the emerging infectious neurologic rat lungworm disease (Jarvi et al 2012). We thus incriminate A. fulica as the likely intermediate host of A. canonensis.…”

Section: Discussionmentioning

confidence: 99%

Parasites of edible land snails in Edo State, Nigeria

et al. 2016

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SummaryLand snails are sources of protein to man and are hosts to a number of parasites. It is imperative that the roles of the snail hosts and parasites are clearly defi ned. Before then however, the parasites of the different land snails collected in any locality should be identifi ed. Land snails were collected in the wild in both dry and wet seasons. The internal organs and the faeces were examined for the presence of parasite. In the rainy season of 2015, a total of 272 snails were collected across four major towns (Benin, Uromi, Ekpoma and Auchi) in Edo State, Nigeria, while in the dry season, fewer snails (n=91) were handpicked. The snail species seen are: Achatina achatina (Linnaeus, 1758), Achatina fulica (Férussac, 1821), Acharchatina marginata (Swainson, 1982), Limicolaria aurora (Jay, 1839), L. fl ammea (Müller, 1774) and Limicolariopsis spp. The larvae of Strongyloides stercoralis were isolated from the various snail species with overall prevalence of 54.04 %. Snails positive with Alaria mesocercariae were L. aurora, L. fl ammea and Limicolariopsis spp. Additionally, few L. fl ammea were positive of the cercariae of Drocoelium dedriticum. Meanwhile, some samples of A. fulica harboured larvae of Angiostrongylus cantonesis, sporocysts of Fasciola gigantica and Schistosoma mansoni. Therefore, these edible snails could pose serious health hazard to man and animals by serving as a possible alternative parasite transmission route.

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“…La meningitis eosinofílica causada por A. cantonensis se diagnostica en gran medida a partir de datos epidemiológicos y de la sospecha clínica, lo que es posible cuando el personal está entrenado y alertado. Para el diagnóstico en el laboratorio se han desarrollado algunas PCR de poca sensibilidad y resultados contradictorios, pruebas que no están disponibles en el comercio (15,24,(27)(28)(29)(30)(31)(32)(33)(34)(35).…”

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Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

2018

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Introducción. En el mundo, las angiostrongilosis de mayor impacto en salud humana y animal son ocasionadas por Angiostrongylus cantonensis, A. costaricensis y A. vasorum. En las personas, las formas clínicas son la meningitis eosinofílica y la angiostrongilosis abdominal, y, en los mamíferos cánidos, el daño cardiopulmonar. Se las consideran enfermedades emergentes debido a la propagación mundial del caracol africano Lissachatina fulica, un huésped intermediario de los parásitos. Los escasos métodos de identificación de Angiostrongylus spp. no son muy específicos ni sensibles y son costosos. Se necesita urgentemente una herramienta diagnóstica asequible, sensible y específicapara el manejo de las angiostrongilosis humana y la animal.Objetivo. Desarrollar una prueba de PCR múltiple en tiempo real (qPCR) para identificar las tres especies patógenas de Angiostrongylus.Materiales y métodos. Mediante un análisis bioinformático se seleccionó una secuencia del genoma ITS-2 de Angiostrongylus para garantizar la especificidad del cebador y las sondas. El ADN de los parásitos adultos (control positivo) y de las larvas se extrajo con el estuche DNeasyBlood & Tissue®.Las reacciones de la PCR cuantitativa se ejecutaron en un termociclador Smartcycler Cepheid®, usando el estuche de mezcla maestra QuantiTect®. Como control negativo, se utilizó ADN humano, de otros parásitos y del caracol africano.Resultados. Los valores del ciclo umbral para los controles positivos de ADN fueron: 21 para Angiostrongylus cantonensis, 22 para A. costaricensis y 31 para A. vasorum. En los controles negativos, el ciclo umbral fue cero. La qPCR mostró una eficiencia de amplificación de 2 (100 %).Conclusiones. En el laboratorio se estandarizó una qPCR múltiple para tres especies clínicamente significativas de Angiostrongylus.

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Quantitative PCR estimates Angiostrongylus cantonensis (rat lungworm) infection levels in semi-slugs (Parmarion martensi)

Cited by 39 publications

References 9 publications

Real-Time Polymerase Chain Reaction Detection of Angiostrongylus cantonensis DNA in Cerebrospinal Fluid from Patients with Eosinophilic Meningitis

Real-Time Polymerase Chain Reaction Detection of Angiostrongylus cantonensis DNA in Cerebrospinal Fluid from Patients with Eosinophilic Meningitis

Parasites of edible land snails in Edo State, Nigeria

Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

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