Quantitative PCR for the detection of genomic DNA of Epstein-Barr virus in ocular fluids of patients with uveitis

Abstract

EBV DNA was detected by qualitative PCR in ocular fluids of many uveitis patients, but only a small proportion of patients showed high viral loads on quantitative real-time PCR, indicating that replication of the virus takes place only in a few patients.

Development of Epstein–barr Virus Uveitis After Remission of Primary Vitreoretinal Lymphoma

“…The virus has also been linked to conjunctivitis, dacryoadenitis, episcleritis, keratitis, iritis, optic neuritis, and uveitis. 6,9,10 Although rare, EBV DNA has been found in intraocular fluids of patients with uveitis. 9,10 Therefore, EBV may be associated with both PVRL and uveitis.…”

A new era of uveitis: impact of polymerase chain reaction in intraocular inflammatory diseases

“…A new era of uveitis: impact of polymerase chain reaction in intraocular inflammatory diseases 5 Corneal endotheliitis and iritis [26] Acute retinal necrosis [27], [11] HHV2 (HSV2) Acute retinal necrosis [43], [27], [11] HHV3 (varicella-zoster virus; VZV) Herpes zoster ophthalmicus/zoster sine herpete [31], [40] Acute retinal necrosis [28], [27], [11] HHV4 (Epstein-Barr virus; EBV) Anterior uveitis/pan-uveitis [55], [54], [53] Acute retinal necrosis [11] Intraocular lymphoma [56] HHV5 (cytomegalovirus; CMV Corneal endotheliitis [36], [35], [7], [37] Iritis (uveitis) [33], [6], [34] Cytomegalovirus retinitis [32] HHV6 Iritis (uveitis) [59], [61], [60] Endophthalmitis [60] Keratitis [61], [63], [60], [62] AIDS-associated retinitis [64] HHV7 Corneal endotheliitis and iritis [66] HHV8 Corneal endotheliitis and iritis [67] Kaposi's sarcoma (conjunctiva, ocular adnexa) [70] HHV1-8 Eight types of human herpes virus, AIDS acquired immune deficiency syndrome a These are representative references by PCR-associated papers…”

“…EBV infects B-lymphocytes and retinal pigment epithelial (RPE) cells [55], leading to the speculation that the intraocular infiltrating B-lymphocytes and RPE cells release the genome within the eye during intraocular inflammation. In addition, EBV DNA is often detected in ocular samples from patients with severe intraocular inflammation or tissue damage, such as ARN [11,56] and intraocular lymphoma [56].…”

Caractéristiques cliniques et paracliniques des uvéites syphilitiques

“…Dans notre étude, nous n'avons pas retrouvé de corrélation entre une augmentation de la sensibilité en cas d'atteinte bilatérale, d'une baisse d'acuité visuelle initiale plus profonde, d'une durée d'évolution plus longue avant l'instauration du traitement, de la présence d'une coinfection VIH ou de signes extra-oculaires associés. Quatre patients sur 8 ont présenté une PCR positive dans l'humeur aqueuse pour le virus Epstein Barr (EBV) pouvant être la conséquence d'un relargage de cet ADN EBV, déjà présent dans l'oeil, par les cellules endommagées par l'inflammation oculaire ou d'une réactivation d'un EBV latent [18].…”