Quantitative phase imaging comparison of digital holographic microscopy and transport of intensity equation phase through simultaneous measurements of live cells

Carney, Shane; Khoo, Ting Chean; Sheikhsofla, Alireza; Ghazanfarpour, Samaneh; Mahajan, Supriya D.; Khmaladze, Alexander; Petruccelli, Jonathan C.

doi:10.1016/j.optlaseng.2023.107581

Cited by 16 publications

(1 citation statement)

References 48 publications

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“…However, Spiegel et al [36] found that FM100 had the potential for droplet loss during sampling from surrounding air and calculated the loss efficiency through theoretical derivation. As a non-contact three-dimensional measurement technology, digital holographic imaging has been successfully used in the field of three-dimensional motion of particles [37][38][39][40][41][42], cloud droplets detection [43,44] and biological cell imaging [45][46][47][48][49]. Madeline et al [43] observed the space structure and droplet size distribution at the smallest turbulence by using airborne holographic imaging.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Investigation of Light Scattering and Digital Holographic Imaging to Measure Liquid Phase Cloud Droplets

Zhang,

Wang,

Yang

et al. 2023

Atmosphere

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The measurement of cloud microphysical parameters plays an important role in describing characteristics of liquid phase clouds and investigating mutual relationships between clouds and precipitation. In this paper, cloud microphysical parameters at Liupan Mountain Weather Station in Ningxia are measured with a high-resolution coaxial digital holographic imager and a fog monitor 120. There are differences in the measurement results between the two instruments. The number concentration measured by the digital holographic imager is about 1.5 times that of the fog monitor 120. However, their Pearson correlation coefficient is above 0.9. Through analysis, we found that the measurement results of the digital holographic imager and fog monitor 120 are differences in 2–4 µm and 7–50µm. For the droplets with the diameters of 4–7 µm, their measurement results have good consistency. By analyzing the influence of wind field and detection sensitivity on the measurement principle, the reasons which caused the difference are proposed. Advice is given to observe topographic clouds by using the above two instruments. In addition, the differences in liquid water content and visibility are analyzed due to the absence of small and large droplets. The study provides data support for improving the accuracy of instruments in measuring cloud droplets and is useful for research in the field of cloud microphysical processes.

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Section: Introductionmentioning

confidence: 99%

A Comparative Investigation of Light Scattering and Digital Holographic Imaging to Measure Liquid Phase Cloud Droplets

Zhang,

Wang,

Yang

et al. 2023

Atmosphere

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Moving grating‐based laser heterodyne digital holographic microscopy system for measuring dynamic phase of living cell attachment

Wang,

Wu,

Zhang

et al. 2023

Journal of Biophotonics

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We propose a laser heterodyne digital holography microscopy system based on a moving grating, which uses the Doppler principle between a moving grating and beam to achieve a low‐frequency bias between the diffracted beams, abandoning traditional heterodyne digital holography that requires multiple acousto‐optic modulators. The dynamic phase distribution obtained using the laser heterodyne digital holography phase‐reconstruction algorithm was more realistic and analyzable than the results of the angular spectrum algorithm. The structure and algorithm were used to capture the shape characteristics of mouse fibroblasts after ~2 h of incubation (37°C, 5% CO2), and the dynamic phase distribution of the cells was monitored in real‐time during the attachment process. The system proposed in this study, with its high spatial resolution and high‐precision phase measurement capability, is suitable for both static and live cells.

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Illumination system contributing zooming function to lensless digital holographic microscope by using lightguide incorporated with volume holographic optical elements

Yu,

Wang,

Chen

et al. 2024

Optics & Laser Technology

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Quantitative phase imaging comparison of digital holographic microscopy and transport of intensity equation phase through simultaneous measurements of live cells

Cited by 16 publications

References 48 publications

A Comparative Investigation of Light Scattering and Digital Holographic Imaging to Measure Liquid Phase Cloud Droplets

A Comparative Investigation of Light Scattering and Digital Holographic Imaging to Measure Liquid Phase Cloud Droplets

Moving grating‐based laser heterodyne digital holographic microscopy system for measuring dynamic phase of living cell attachment

Illumination system contributing zooming function to lensless digital holographic microscope by using lightguide incorporated with volume holographic optical elements

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