Quantitative phosphoproteomics of protein kinase SnRK1 regulated protein phosphorylation in Arabidopsis under submergence

Cho, Hsing-Yi; Teng, Wen; Wang, Ying-Tsui; Shih, Ming-Che

doi:10.1093/jxb/erw107

Cited by 112 publications

(113 citation statements)

References 63 publications

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“…An Arabidopsis SnRK1.1 dominant-negative mutant showed lower tolerance to submergence (Cho et al, 2016). However, overexpression of the SnRK1 subfamily members KIN10 as well as the dominant-negative mutation in SnRK1.1 had no consequences on the starvation-dependent dampening of ADH expression (Fig.…”

Section: Discussionmentioning

confidence: 93%

“…8A). We then tested the dominant-negative SnRK1.1 K48M line (Cho et al, 2016). The results showed that C starvation repressed ADH expression also in SnRK1.1 K48M , suggesting that the starvation-dependent effects of ADH expression are independent of SnrK1.1 (Fig.…”

Section: Sugar Starvation Dampens the Anaerobic Response Upstream Of mentioning

confidence: 99%

“…The quintuple erfVII mutant (Marín-de la ) is a null mutant for RAP2.2, RAP2.3, RAP2.12, HRE1, HRE2, and a knockdown for RAP2.2 (Giuntoli et al, 2017b); a line constitutively expressing a version of the RAP2.12 protein that is also stable in air (35S:D-RAP2.12; Licausi et al, 2011); a line expressing GUS under the control of the PCO1 promoter (pPCO1:GUS; Weits et al, 2014); and a line overexpressing HRA1 . The bzip63 mutant and the bZIP63 overexpressor (35S:bZIP63) (Mair et al, 2015), two independent KIN10 overexpressor lines (OE1 KIN10 and OE2 KIN10) (Baena-González et al, 2007); the Arabidopsis SnRK1.1 dominant-negative mutant (SnRK1.1 K48M ; Cho et al, 2016); a line expressing RAP2.3 with an HA tag (35S:RAP2.3-HA; Gibbs et al, 2014).…”

Section: Plant Materialsmentioning

confidence: 99%

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Gene Regulation and Survival under Hypoxia Requires Starch Availability and Metabolism

et al. 2017

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Plants respond to hypoxia, often caused by submergence, by expressing a specific set of genes that contribute to acclimation to this unfavorable environmental condition. Genes induced by low oxygen include those encoding enzymes for carbohydrate metabolism and fermentation, pathways that are required for survival. Sugar availability is therefore of crucial importance for energy production under hypoxia. Here, we show that Arabidopsis (Arabidopsis thaliana) plants require starch for surviving submergence as well as for ensuring the rapid induction of genes encoding enzymes required for anaerobic metabolism. The starchless pgm mutant is highly susceptible to submergence and also fails to induce anaerobic genes at the level of the wild type. Treating wild-type plants under conditions inducing sugar starvation results in a weak induction of alcohol dehydrogenase and other anaerobic genes. Induction of gene expression under hypoxia requires transcription factors belonging to group VII ethylene response factors (ERF-VII) that, together with plant Cys oxidases, act as an oxygen-sensing mechanism. We show that repression of this pathway by sugar starvation occurs downstream of the hypoxia-dependent stabilization of ERF-VII proteins and independently of the energy sensor protein kinases SnRK1

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Section: Discussionmentioning

confidence: 93%

Section: Sugar Starvation Dampens the Anaerobic Response Upstream Of mentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

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Gene Regulation and Survival under Hypoxia Requires Starch Availability and Metabolism

et al. 2017

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“…In fact, evidence for this scenario was provided recently by the finding that ABI4, a central TF involved in different chloroplast retrograde signaling pathways, was phosphorylated by the MAP kinase MPK6 in a Ca 2+ -dependent manner (Guo et al, 2016). Strikingly, MPK6 also was recently found to be regulated by SnRK1 under hypoxia conditions (Cho et al, 2016).…”

Section: Anterograde Signals To Organelles Via Phosphorylation Of Tramentioning

confidence: 99%

“…SnRK1 activation also is triggered by hypoxia, leading to suppression of mitochondrial aerobic respiration resulting in cellular energy deprivation (Im et al, 2014;Cho et al, 2016). Flooding stress or hypoxia leads to a reduced availability of O 2 as the final electron acceptor in the mitochondrial electron transport chain (mETC), causing a rapid decrease in the cellular ATP:ADP ratio (Bailey-Serres and Voesenek, 2008).…”

Section: Snrk1 In Metabolic and Hormone Signaling Under Stress Conditmentioning

confidence: 99%

The SnRK1 Kinase as Central Mediator of Energy Signaling between Different Organelles

et al. 2018

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The evolutionarily conserved SNF1-related protein kinase 1 (SnRK1) kinase complex is a key regulator in adjusting cellular metabolism during starvation, stress conditions, and growth-promoting conditions. Over the last two decades, extensive genetic evidence for a widespread SnRK1 signaling network has accumulated. It is now well established that SnRK1 is a central integrator of energy signaling. However, little is known about the connections between the cytoplasmic and nuclear-localized SnRK1 and plastids and mitochondria as the main energy-producing compartments in the cell. Here, we review recent findings indicating how SnRK1 affects metabolic adaptation, including plastidial and mitochondrial functions. Special emphasis is put on identified direct targets of SnRK1, which would eventually enable cross talk with organelles. In this context, a number of transcription factors (TFs) are emerging as mediators of SnRK1 signaling, potentially linking SnRK1 activity to organellar functions. Furthermore, many SnRK1 targets act in various hormonal signaling pathways, which are at least partly localized in plastids. With this review, we summarize the current knowledge on SnRK1 organelle interaction and provide ideas on the potential molecular mechanisms governing these interactions. METABOLIC REPROGRAMMING BY SNRK1 KINASE ACTIVITY UNDER DIFFERENT GROWTH AND STRESS CONDITIONSAlready under optimal growth conditions, plants need to continuously adjust their metabolic balance between autotrophic growth based on photosynthesis during the day and respiration during the night. At daytime, sugars and other metabolites are produced by photosynthesis in chloroplasts and further distributed to be used in other metabolic pathways at different cellular compartments or transported to nonphotosynthetic sink tissues. During the night, starch and sugars supply carbon equivalents for respiration, providing the necessary energy for further growth and metabolic activities. Additionally, metabolic reprogramming is required for plants to adjust their metabolism to diverse biotic and abiotic environmental stimuli. This often leads to a stop of plant growth involving a reduction in ribosomal protein synthesis, and in parallel, accumulation of protective metabolites or defense compounds. This switching of cellular energy metabolism is mediated by the activity of the evolutionarily conserved AMPK/ SNF1/SnRK1 kinase complex (Box 1; Crozet et al.,

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Redox state‐dependent modulation of plant SnRK1 kinase activity differs from AMPK regulation in animals

et al. 2017

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The evolutionarily highly conserved SNF1‐related protein kinase (SnRK1) protein kinase is a metabolic master regulator in plants, balancing the critical energy consumption between growth‐ and stress response‐related metabolic pathways. While the regulation of the mammalian [AMP‐activated protein kinase (AMPK)] and yeast (SNF1) orthologues of SnRK1 is well‐characterised, the regulation of SnRK1 kinase activity in plants is still an open question. Here we report that the activity and T‐loop phosphorylation of AKIN10, the kinase subunit of the SnRK1 complex, is regulated by the redox status. Although this regulation is dependent on a conserved cysteine residue, the underlying mechanism is different to the redox regulation of animal AMPK and has functional implications for the regulation of the kinase complex in plants under stress conditions.

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Quantitative phosphoproteomics of protein kinase SnRK1 regulated protein phosphorylation in Arabidopsis under submergence

Abstract: HighlightTo illustrate the hypoxia signal mediated by SnRK1.1, phosphoproteomics was used to identify not only the new SnRK1.1 putative targets related to submergence, but also to gain an insight into early hypoxia signalling events.

Cited by 112 publications

References 63 publications

Gene Regulation and Survival under Hypoxia Requires Starch Availability and Metabolism

Gene Regulation and Survival under Hypoxia Requires Starch Availability and Metabolism

The SnRK1 Kinase as Central Mediator of Energy Signaling between Different Organelles

Redox state‐dependent modulation of plant SnRK1 kinase activity differs from AMPK regulation in animals

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