Quantitative Polarization Imaging of Zebrafish Muscle Structures

Polarization-sensitive Fourier-ptychography microscopy (pFPM) allows for high resolution imaging while maintaining a large field of view, and without mechanical movements of optical-setup components. In contrast to ordinary light microscopes, pFPM provides quantitative absorption and phase information, for complex and birefringent specimens, with high resolution across a wide field of view. Using a semi-spherical home-built LED illumination array, a single polarizer, and a 10x /0.28NA objective, we experimentally demonstrate high performance pFPM with a synthesized NA of 1.1. Applying the standard quantitative method, a measured half-pitch resolution of 244 nm is achieved for the 1951 USAF resolution test target. As application examples, the polarimetric properties of a herbaceous flowering plant and the metastatic carcinoma of human liver cells are analyzed and quantitatively imaged.

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High-resolution polarization-sensitive Fourier ptychography microscopy using a high numerical aperture dome illuminator

GholamiMayani¹,

Tekseth

Breiby

et al. 2022

Opt. Express

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Quantitative Polarization Imaging of Zebrafish Muscle Structures

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High-resolution polarization-sensitive Fourier ptychography microscopy using a high numerical aperture dome illuminator

High-resolution polarization-sensitive Fourier ptychography microscopy using a high numerical aperture dome illuminator

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