Quantitative prevalence and characterization of Campylobacter from chicken and duck carcasses from poultry slaughterhouses in South Korea

Chon, Jung‐Whan; Lee, Soo-Kyung; Yoon, Yohan; Yoon, Ki Sun; Kwak, Hyo‐Sun; Joo, In-Sun; Seo, Kun‐Ho

doi:10.3382/ps/pey120

Cited by 15 publications

(12 citation statements)

References 32 publications

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“…In this study, a high rate of Campylobacter was detected in 240 (28.40%) isolates from meat carcasses, and this gives valuable insight into the possible risks of foodborne infection to humans. Studies carried out in Italy by Stella et al [55], in Malaysia [56], in China [57], in France [58], and in South Korea [59] also detected Campylobacter in meat samples with detection rates of 34.10%, 50.9%, 48.9%, 76%, and 31.67%, respectively, and our findings are in line with their reports. Other studies carried out in Spain by García-Sánchez et al [60], in Pakistan by Nisar et al [3], in Yangzhou, China by Zou et al [61], and in Northern Poland by Andrzejewska et al [62] also detected Campylobacter in meat samples, and these results are also akin with their reports.…”

Section: Discussionsupporting

confidence: 92%

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

Igwaran

Okoh

2020

Foods

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Raw meats are sometimes contaminated with Campylobacter species from animal faeces, and meats have repeatedly been implicated in foodborne infections. This study evaluated the prevalence, virulence genes, antimicrobial susceptibility patterns, and resistance gene determinants in Campylobacter species isolated from retailed meat carcasses. A total of 248 raw meat samples were collected from butcheries, supermarkets, and open markets; processed for enrichment in Bolton broth; and incubated at 42 °C for 48 h in 10% CO2. Thereafter, the broths were streaked on modified charcoal cefoperazone deoxycholate agar (mCCDA) plates and incubated at the same conditions and for the same amount of time. After incubation, colonies were isolated and confirmed by Polymerase chain reaction using specific oligonucleotide sequences used for the identification of the genus Campylobacter, species, and their virulence markers. The patterns of antimicrobial resistance profiles of the identified isolates were studied by disk diffusion method against 12 antibiotics, and relevant resistance genes were assessed by PCR. From culture, 845 presumptive Campylobacter isolates were obtained, of which 240 (28.4%) were identified as genus Campylobacter. These were then characterised into four species, of which C. coli had the highest prevalence rate (22.08%), followed by C. jejuni (16.66%) and C. fetus (3.73%). The virulence genes detected included iam (43.14%), cadF (37.25%), cdtB (23.53%), flgR (18.63%), and flaA (1.96%), and some of the isolates co-harboured two to four virulence genes. Of the 12 antibiotics tested, the highest phenotypic resistance displayed by Campylobacter isolates was against clindamycin (100%), and the lowest level of resistance was observed against imipenem (23.33%). The frequency of resistance genes detected included catll (91.78%), tetA (68.82%), gyra (61.76%), ampC (55%), aac(3)-IIa (aacC2)a (40.98%), tetM (38.71%), ermB (18.29%), tetB (12.90%), and tetK (2.15%). There is a high incidence of Campylobacter species in meat carcasses, suggesting these to be a reservoir of campylobacteriosis agents in this community, and as such, consumption of undercooked meats in this community is a potential health risk to consumers.

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Section: Discussionsupporting

confidence: 92%

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

Igwaran

Okoh

2020

Foods

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“…According to statistics provided by the Animal and Plant Quarantine Agency of Korea 7 (accessed on May 2019) over the last 2 years, the antibiotic-resistance rates of poultry-derived C. jejuni against ciprofloxacin, nalidixic acid, and tetracycline were 97.4, 94.7, and 63.2%, respectively. In addition, almost all C. jejuni isolates from humans and livestock in Korea were resistant to at least one antibiotic (Kim et al, 2016;Chon et al, 2018). However, in this study, the antibiotic-resistance patterns of C. jejuni from wild mice deviated from this pattern.…”

Section: Discussioncontrasting

confidence: 65%

The Wild Mouse (Micromys minutus): Reservoir of a Novel Campylobacter jejuni Strain

et al. 2020

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Campylobacter jejuni is one of the most common zoonotic pathogens worldwide. Although the main sources of human C. jejuni infection are livestock, wildlife can also affect C. jejuni transmission in humans. However, it remains unclear whether wild mice harbor C. jejuni and are involved in the "environment-wildlife-livestock-human" transmission cycle of C. jejuni in humans. Here, we characterized C. jejuni from wild mice and identified genetic traces of wild mouse-derived C. jejuni in other hosts using a traditional approach, along with comparative genomics. We captured 115 wild mice (49 Mus musculus and 66 Micromys minutus) without any clinical symptoms from 22 sesame fields in Korea over 2 years. Among them, M. minutus were typically caught in remote areas of human houses and C. jejuni was solely isolated from M. minutus (42/66, 63.6%). We identified a single clone (MLST ST-8388) in all 42 C. jejuni isolates, which had not been previously reported, and all isolates had the same virulence/survival-factor profile, except for the plasmid-mediated virB11 gene. No isolates exhibited antibiotic resistance, either in phenotypic and genetic terms. Comparative-genomic analysis and MST revealed that C. jejuni derived from M. minutus (strain SCJK2) was not genetically related to those derived from other sources (registered in the NCBI genome database and PubMLST database). Therefore, we hypothesize that C. jejuni from M. minutus is a normal component of the gut flora following adaptation to the gastro-intestinal tract. Furthermore, M. minutus-derived C. jejuni had different ancestral lineages from those derived from other sources, and there was a low chance of C. jejuni transmission from M. minutus to humans/livestock because of their habitat. In conclusion, M. minutus may be a potential reservoir for a novel C. jejuni, which is genetically different from those of other sources, but may not be involved in the transmission of C. jejuni to other hosts, including humans and livestock. This study could form the basis for further studies focused on understanding the transmission cycle of C. jejuni, as well as other zoonotic pathogens originating from wild mice.

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“…mCCD agar showed greater efficacy to enumerate Campylobacter than to isolate the bacterium after 24-h enrichment in Bolton broth ( P < 0.0001) in concurrently analyzed samples. Similar to our findings, Chon et al. (2018) detected higher Campylobacter prevalence rates in poultry meat samples by an enumerative assay than by a qualitative analysis with a selective enrichment step.…”

Section: Resultssupporting

confidence: 91%

Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil

et al. 2020

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Chicken is a leading source of thermotolerant Campylobacter , which triggers human foodborne enteritis. This study evaluated thermotolerant Campylobacter contamination of retail chicken in southern Brazil, using qualitative and quantitative analyses. Selective enrichment in Bolton broth for 24 and 48 h after plating onto modified charcoal–cefoperazone–deoxycholate ( mCCD ) agar and Preston agar was assessed. The combined results of the detection and enumeration methods revealed a frequency of 70% occurrence of thermotolerant Campylobacter in chicken samples. Campylobacter was enumerated in 60% of the samples, whereas 46% of the samples were positive in the qualitative analysis. Quantitative analysis showed average counts of 3.10 ± 0.15 log 10 CFU/sample. Higher numbers of Campylobacter -positive samples were found using 24-h enrichment before plating onto Preston agar (46%) than onto mCCD agar (2%). The majority of isolated strains were identified as Campylobacter jejuni , and Campylobacter coli was also found but to a lesser extent. Subtyping revealed a clear distinction between strains isolated from different chicken sources. The enriched samples plated onto mCCD agar showed extensive spreading of nonproducing extended-spectrum β-lactamases Proteus mirabilis that hampered the identification of Campylobacter colonies. P. mirabilis strains showed resistance to cefoperazone, trimethoprim, and polymyxin B present in broth and plate media used and were inhibited by rifampicin present in Preston agar. The results underline the effect of the spread of contaminant strains on Campylobacter cultures, which might be prevented using a recently revised International Organization for Standardization method for qualitative analysis of chicken.

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Quantitative prevalence and characterization of Campylobacter from chicken and duck carcasses from poultry slaughterhouses in South Korea

Cited by 15 publications

References 32 publications

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

The Wild Mouse (Micromys minutus): Reservoir of a Novel Campylobacter jejuni Strain

Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil

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