Quantitative profiling of 19 bile acids in rat plasma, liver, bile and different intestinal section contents to investigate bile acid homeostasis and the application of temporal variation of endogenous bile acids

Yang, Tingting; Shu, Ting; Liu, Guanlan; Mei, Huifang; Zhu, Xiaoyu; Huang, Xin; Zhang, Luyong; Jiang, Zhenzhou

doi:10.1016/j.jsbmb.2017.05.015

Cited by 123 publications

(72 citation statements)

References 41 publications

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“…3A, B and C). As previously described, Yang et al [20] recently determined taurine conjugated bile acids to be the most abundant type in bile from rats, whereas the unconjugated cholic acid play a dominant role in the small intestine. As seen in Fig.…”

Section: Maldi Msi On Sections From the Small Intestinementioning

confidence: 77%

“…The most abundant bile acids in humans have recently been determined to be glyco-conjugates [19]. In contrast, Yang et al [20] quantitatively investigated GI fluid from rats and found taurocholic acid to be the most abundant bile acid in the bile duct, whereas the unconjugated cholic acid was mainly present in the small intestine. However, more information is needed about the types of bile acids and phospholipids present in the GI tract of rats.…”

Section: Bile Acids (Mm)mentioning

confidence: 98%

See 1 more Smart Citation

Developing a predictive in vitro dissolution model based on gastrointestinal fluid characterisation in rats

Christfort

Strindberg

Plum

et al. 2019

European Journal of Pharmaceutics and Biopharmaceutics

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Section: Maldi Msi On Sections From the Small Intestinementioning

confidence: 77%

Section: Bile Acids (Mm)mentioning

confidence: 98%

Developing a predictive in vitro dissolution model based on gastrointestinal fluid characterisation in rats

Christfort

Strindberg

Plum

et al. 2019

European Journal of Pharmaceutics and Biopharmaceutics

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“…Several earlier reports indicated that the plasma concentrations of endogenous BAs were affected by the circadian rhythm, the food intake and other major physiological processes (Dekaney, von Allmen, Garrison, Rigby, & Lund, 2008; Eggink et al, 2017; Yamada et al, 2000; Yamajuku et al, 2009; Yang et al, 2017). In our preliminary experiment, we observed that the BA plasma concentrations appeared to be more stable around noon and in the afternoon within one day, and stimulation (e.g.…”

Section: Resultsmentioning

confidence: 99%

A validated surrogate analyte UPLC–MS/MS assay for quantitation of TUDCA, TCDCA, UDCA and CDCA in rat plasma: Application in a pharmacokinetic study of cultured bear bile powder

Zan

Xin-hua

Zhao

et al. 2020

Biomedical Chromatography

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Bear bile is a valuable medicinal material used in traditional Chinese medicine for over 2000 years. However, developing a substitute has become necessary because of protection measures for this endangered species. The ingredients of in vitro cultured bear bile powder (CBBP) include tauroursodeoxycholic acid (TUDCA), taurochenodeoxycholic acid (TCDCA), ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA, and it has pharmacological properties that are similar to those of natural bear bile powder (NBBP). In this study, the pharmacokinetic parameters of both CBBP and NBBP were measured in rats with a new surrogate analyte LC–MS method using stable isotopes as surrogate analytes (D4‐TUDCA, D4‐TCDCA, D4‐UDCA and D4‐CDCA) with response factors validated in authentic matrix (plasma) for simultaneously monitoring the authentic analytes (TUDCA, TCDCA, UDCA and CDCA). The method validation was satisfactory for the linear regression (r, 0.9975–0.9994), precision (RSD intra‐day, 0.72–9.35%; inter‐day, 3.82–9.02%), accuracy (RE, −12.42–5.67%) and matrix effect (95.53–99.80%), along with analyte recovery (95.90–98.82%) and stability (89.48–101.81%) of surrogate analytes, and precision (RSD intra‐day, 1.06– 11.51%; inter‐day, 2.23– 11.38%), accuracy (RE, −7.40–10.76%) and stability (87.37–111.70%) of authentic analytes. We successfully applied this method to evaluate the pharmacokinetics of CBBP and NBBP in rats, which revealed the critical in vivo properties of both bear bile preparations.

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“…The synthesis of BAs is the main elimination pathway for cholesterol, and a growing number of studies have shown that BAs homeostasis played an important regulatory role in lipid metabolism (D. Q. Wang, Neuschwander‐Tetri, Portincasa, & Pandak, ; Yang et al, ; Yu, Jiang, & Zhang, ). Our study revealed that the dysregulated BA homeostasis was attenuated by quercetin in T2DM‐induced NAFLD.…”

Section: Discussionmentioning

confidence: 99%

Quercetin improves nonalcoholic fatty liver by ameliorating inflammation, oxidative stress, and lipid metabolism in db/db mice

Yang

Cai

et al. 2019

Phytotherapy Research

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189

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Multiphase pathological processes involve in Type 2 diabetes (T2DM)‐induced nonalcoholic fatty liver disease (NAFLD). However, the therapies are quite limited. In the present study, the hepatoprotective effects and underlying mechanisms of quercetin in T2DM‐induced NAFLD were investigated. T2DM‐induced NAFLD and quercetin treatment models were established in vivo and in vitro. The results revealed that quercetin alleviated serum transaminase levels and markedly reduced T2DM‐induced histological alterations of livers. Additionally, quercetin restored superoxide dismutase, catalase, and glutathione content in livers. Not only that, quercetin markedly attenuated T2DM‐induced production of interleukin 1 beta, interleukin 6, and TNF‐α. Accompanied by the restoration of the increased serum total bile acid (p = .0001) and the decreased liver total bile acid (p = .0005), quercetin could reduce lipid accumulation in the liver of db/db mice. Further mechanism studies showed that farnesoid X receptor 1/Takeda G‐protein‐coupled receptor 5 signaling pathways was involved in quercetin regulation of lipid metabolism in T2DM‐induced NAFLD. In high D‐glucose and free fatty acid cocultured HepG2 cells model, quercetin eliminated lipid droplets and restored the upregulated total cholesterol and triglyceride levels. Similar to the findings in mice, quercetin could also activate farnesoid X receptor 1/Takeda G‐protein‐coupled receptor 5 signaling pathway. These findings suggested that quercetin might be a potentially effective drug for the treatment of T2DM‐induced NAFLD.

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Quantitative profiling of 19 bile acids in rat plasma, liver, bile and different intestinal section contents to investigate bile acid homeostasis and the application of temporal variation of endogenous bile acids

Cited by 123 publications

References 41 publications

Developing a predictive in vitro dissolution model based on gastrointestinal fluid characterisation in rats

Developing a predictive in vitro dissolution model based on gastrointestinal fluid characterisation in rats

A validated surrogate analyte UPLC–MS/MS assay for quantitation of TUDCA, TCDCA, UDCA and CDCA in rat plasma: Application in a pharmacokinetic study of cultured bear bile powder

Quercetin improves nonalcoholic fatty liver by ameliorating inflammation, oxidative stress, and lipid metabolism in db/db mice

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