Quantitative profiling of glucosinolates by LC–MS analysis reveals several cultivars of cabbage and kale as promising sources of sulforaphane

Sasaki, Katsunori; Neyazaki, Makiko; Shindo, Kazutoshi; Ogawa, Takayoshi; Momose, Masaki

doi:10.1016/j.jchromb.2012.07.017

Cited by 48 publications

(33 citation statements)

References 30 publications

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“…It should be highlighted that different methods for analyzing GLSs, in accordance with the presence (intact or non-intact desulfo-derivatives) of a sulfate group, have been reported in the literature. Regarding the analysis of intact GLSs, solid-phase extraction has been successfully used to extract intact GLSs with other matrices, especially vegetables (Rochfort et al 2006;Sasaki et al 2012); similar results have been seen with pressurized liquid extraction (Alarcón-Flores et al 2013;Mohn et al 2007). Nevertheless, the preferable and simplest choice is a solvent extraction with heated water or mixtures of methanol and water (Ares et al 2014;Bell et al 2015;Domínguez-Perles et al 2010;Glauser et al 2012;López-Berenguer et al 2008;Millán et al 2009;Rochfort et al 2008).…”

Section: Introductionmentioning

confidence: 63%

“…Consequently, taking into account our experience and the favorable results obtained in recent research (Ares et al 2015;Yáñez et al 2014), we decided to use solvent extraction as the sample treatment. Analysis of intact GLSs was usually performed by LC with ultraviolet or diode array (Ares et al 2014;Gratacós-Cubarsí et al 2010;López-Berenguer et al 2008;Rochfort et al 2006;Truchado et al 2010;) and mass spectrometry (MS) (Alarcón-Flores et al 2013;Ares et al 2014;Bell et al 2015;Domínguez-Perles et al 2010;Glauser et al 2012;López-Berenguer et al 2008;Millán et al 2009;Rochfort et al 2008;Sasaki et al 2012) detectors, while C 18 analytical columns were used in most cases. Among these, MS, and in particular tandem mass spectrometry (MS/MS), has emerged as one of the preferred detection techniques, providing as it does sufficient sensitivity and unambiguous identification and quantification of GLSs (Alarcón-Flores et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Intact Glucosinolates in Beeswax by Liquid Chromatography Tandem Mass Spectrometry

2015

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A new method was developed to determine 12 intact glucosinolates (glucoiberin, glucoraphanin, glucoerucin, gluconapin, glucotropaeolin, glucobrassicin, gluconasturtiin, glucoalyssin, 4-hydroxyglucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin, sinigrin) in beeswax, which is an authorized food additive in the European Union, by means of liquid chromatography tandem mass spectrometry with electrospray ionization. An efficient extraction procedure was proposed (average analyte recoveries were between 88 and 101 %); this involved the dissolution of melted beeswax with hexane, a solvent extraction using a mixture of ethanol and water (80:20, v/v), and a concentration step in a rotary evaporator. Chromatography was performed on a C 18 analytical column with a mobile phase of formic acid in water (0.5 %, v/v) and formic acid in acetonitrile (0.5 %, v/v), at a flow rate of 1 mL/min. The method was fully validated in terms of selectivity, limits of detection and quantification, linearity, precision, and accuracy. The limits of detection and quantification values were below 7 and 23 μg/kg, respectively. The proposed method was applied to analyze beeswax samples collected from organic apiaries.

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Section: Introductionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Analysis of Intact Glucosinolates in Beeswax by Liquid Chromatography Tandem Mass Spectrometry

2015

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“…2), but different proportions of them have been observed in different studies. Generally, glucoraphanin is present in low amount; however, some kale varieties, such as black kale, can contain high glucoraphanin level [90].…”

Section: 9mentioning

confidence: 99%

Glucosinolates in Food

et al. 2016

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“…This is not easy to answer for the following reasons. First, the bioavailability of sulforaphane upon cabbage consumption is highly variable among different consumers [46]. Second, the presence of glucoraphanin in different Brassica oleracea varieties is highly variable [46].…”

Section: Broccoli Is a Rich Source Of Sulforaphanementioning

confidence: 99%

“…First, the bioavailability of sulforaphane upon cabbage consumption is highly variable among different consumers [46]. Second, the presence of glucoraphanin in different Brassica oleracea varieties is highly variable [46]. The variation of glucoraphanin in six cultivars of broccoli ranged from 12.2 to 119.4 mg/100 g fresh weight, with an average of 60 mg/100 g serving.…”

Section: Broccoli Is a Rich Source Of Sulforaphanementioning

confidence: 99%

Sulforaphane and related mustard oils in focus of cancer prevention and therapy

Herr¹,

Lozanovski

Houben

et al. 2012

Wien Med Wochenschr

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The plant family Brassicaceae, formerly Cruciferae, contains mustard oil glycosides, from which mustard oils are enzymatically hydrolyzed. Mustard oils offer protection from pests, microorganisms and fungi. More than 120 different mustard oils with various biological functions are known. Since ancient times, these substances are used as natural antibiotics, antiviral drugs and antimycotics. The antioxidative effect of mustard oils contributes to protection from DNA damage. Epidemiological and experimental studies have shown preventive and therapeutic effects of crucifers or isolated substances thereof. Particularly well studied is the mustard oil sulforaphane, which is contained in high concentrations in broccoli and its sprouts. As has been shown in mice recently, sulforaphane also targets the most malignant cancer stem cells, which are not affected by conventional cancer treatments. Based on these promising results, the first prospective clinical studies with cancer patients and sulforaphane-enriched broccoli sprouts have now been initiated in the United States.

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Quantitative profiling of glucosinolates by LC–MS analysis reveals several cultivars of cabbage and kale as promising sources of sulforaphane

Cited by 48 publications

References 30 publications

Analysis of Intact Glucosinolates in Beeswax by Liquid Chromatography Tandem Mass Spectrometry

Analysis of Intact Glucosinolates in Beeswax by Liquid Chromatography Tandem Mass Spectrometry

Glucosinolates in Food

Sulforaphane and related mustard oils in focus of cancer prevention and therapy

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