Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a PHOSPHOLIPASE Dα1 knockout mutant

Devaiah, Shivakumar P.; Roth, Mary R.; Baughman, Ethan; Li, Maoyin; Tamura, Pamela; Jeannotte, Richard; Welti, Ruth; Wang, Xuemin

doi:10.1016/j.phytochem.2006.06.005

Cited by 271 publications

(314 citation statements)

References 29 publications

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“…Lipid samples were analyzed on a triple quadruple MS/MS equipped for ESI. Data processing was performed as previously described (66). The lipids in each class were quantified in comparison with two internal standards of the class.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Sun

Hicks

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The vacuole is the most prominent compartment in plant cells and is important for ion and protein storage. In our effort to search for key regulators in the plant vacuole sorting pathway, ribosomal large subunit 4 (rpl4d) was identified as a translational mutant defective in both vacuole trafficking and normal development. Polysome profiling of the rpl4d mutant showed reduction in polysome-bound mRNA compared with wild-type, but no significant change in the general mRNA distribution pattern. Ribsomal profiling data indicated that genes in the lipid metabolism pathways were translationally down-regulated in the rpl4d mutant. Live imaging studies by Nile red staining suggested that both polar and nonpolar lipid accumulation was reduced in meristem tissues of rpl4d mutants. Pharmacological evidence showed that sterol and sphingolipid biosynthetic inhibitors can phenocopy the defects of the rpl4d mutant, including an altered vacuole trafficking pattern. Genetic evidence from lipid biosynthetic mutants indicates that alteration in the metabolism of either sterol or sphingolipid biosynthesis resulted in vacuole trafficking defects, similar to the rpl4d mutant. Tissue-specific complementation with key enzymes from lipid biosynthesis pathways can partially rescue both vacuole trafficking and auxin-related developmental defects in the rpl4d mutant. These results indicate that lipid metabolism modulates auxin-mediated tissue differentiation and endomembrane trafficking pathways downstream of ribosomal protein function.

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Section: Discussionmentioning

confidence: 99%

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Sun

Hicks

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…For MS analysis, extracts were prepared and analyzed in a method adapted from Devaiah et al (2006). Extracts were analyzed on a triple quadrupole Applied Biosytems/Sciex API 3000 mass spectrometer.…”

Section: Esi-ms Of Transgenic Linesmentioning

confidence: 99%

Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil

et al. 2017

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ORCID IDs: 0000-0001-6929-7859 (S.M.); 0000-0003-3152-0394 (D.S.); 0000-0001-8255-3255 (C.H.); 0000-0003-0489-3072 (K.C.); 0000-0002-9888-7003 (I.F.).Acyltransferases are key contributors to triacylglycerol (TAG) synthesis and, thus, are of great importance for seed oil quality. The effects of increased or decreased expression of ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) or PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE (PDAT) on seed lipid composition were assessed in several Camelina sativa lines. Furthermore, in vitro assays of acyltransferases in microsomal fractions prepared from developing seeds of some of these lines were performed. Decreased expression of DGAT1 led to an increased percentage of 18:3n-3 without any change in total lipid content of the seed. The tri-18:3 TAG increase occurred predominantly in the cotyledon, as determined with matrix-assisted laser desorption/ionization-mass spectrometry, whereas species with two 18:3n-3 acyl groups were elevated in both cotyledon and embryonal axis. PDAT overexpression led to a relative increase of 18:2n-6 at the expense of 18:3n-3, also without affecting the total lipid content. Differential distributions of TAG species also were observed in different parts of the seed. The microsomal assays revealed that C. sativa seeds have very high activity of diacylglycerol-phosphatidylcholine interconversion. The combination of analytical and biochemical data suggests that the higher 18:2n-6 content in the seed oil of the PDAT overexpressors is due to the channeling of fatty acids from phosphatidylcholine into TAG before being desaturated to 18:3n-3, caused by the high activity of PDAT in general and by PDAT specificity for 18:2n-6. The higher levels of 18:3n-3 in DGAT1-silencing lines are likely due to the compensatory activity of a TAG-synthesizing enzyme with specificity for this acyl group and more desaturation of acyl groups occurring on phosphatidylcholine.

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“…Lipid analysis by automated ESI-MS/MS, data acquisition, data analysis and acyl group identification were performed as described previously (19,20), with modifications (SI Methods). To determine the absolute amount of various phospholipid classes, the total lipid extract was combined with internal standards, quantified as previously described (21).…”

Section: Lipid Profiling By Electrospray Ionization-tandem Mass Spectmentioning

confidence: 99%

Metabolic labeling and direct imaging of choline phospholipids in vivo

Jao

Roth

Welti

2009

Proc. Natl. Acad. Sci. U.S.A.

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Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a PHOSPHOLIPASE Dα1 knockout mutant

Cited by 271 publications

References 29 publications

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil

Metabolic labeling and direct imaging of choline phospholipids in vivo

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