Quantitative profiling of polar primary metabolites of two chickpea cultivars with contrasting responses to salinity

Dias, Daniel A.; Hill, Camilla Beate; Jayasinghe, Nirupama S.; Atieno, Judith; Sutton, Tim; Roessner, Ute

doi:10.1016/j.jchromb.2015.07.002

Cited by 88 publications

(81 citation statements)

References 22 publications

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“…Sucrose and inositol were quantified as described in Dias et al . (). Calculated concentrations (concentrations based on response of standards and their expected concentrations) were exported, and the final concentrations were expressed in m m on a FW basis.…”

Section: Methodsmentioning

confidence: 97%

Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

Kiani‐Pouya

Roessner

Jayasinghe

et al. 2017

Plant Cell & Environment

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Epidermal bladder cells (EBCs) have been postulated to assist halophytes in coping with saline environments. However, little direct supporting evidence is available. Here, Chenopodium quinoa plants were grown under saline conditions for 5 weeks. One day prior to salinity treatment, EBCs from all leaves and petioles were gently removed by using a soft cosmetic brush and physiological, ionic and metabolic changes in brushed and non-brushed leaves were compared. Gentle removal of EBC neither initiated wound metabolism nor affected the physiology and biochemistry of control-grown plants but did have a pronounced effect on salt-grown plants, resulting in a salt-sensitive phenotype. Of 91 detected metabolites, more than half were significantly affected by salinity. Removal of EBC dramatically modified these metabolic changes, with the biggest differences reported for gamma-aminobutyric acid (GABA), proline, sucrose and inositol, affecting ion transport across cellular membranes (as shown in electrophysiological experiments). This work provides the first direct evidence for a role of EBC in salt tolerance in halophytes and attributes this to (1) a key role of EBC as a salt dump for external sequestration of sodium; (2) improved K retention in leaf mesophyll and (3) EBC as a storage space for several metabolites known to modulate plant ionic relations.

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Section: Methodsmentioning

confidence: 97%

Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

Kiani‐Pouya

Roessner

Jayasinghe

et al. 2017

Plant Cell & Environment

Self Cite

119

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“…Metabolite data extraction and analysis were undertaken based on the protocol described in Dias et al . (). Compound identification was based on an in‐house library of MS spectra, retention indices (based on n ‐alkane standards) and fatty acid standards (Menhaden fish oil; Sigma‐Aldrich).…”

Section: Methodsmentioning

confidence: 97%

Mapping carbon fate during bleaching in a model cnidarian symbiosis: the application of ¹³C metabolomics

et al. 2017

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Coral bleaching is a major threat to the persistence of coral reefs. Yet we lack detailed knowledge of the metabolic interactions that determine symbiosis function and bleaching-induced change. We mapped autotrophic carbon fate within the free metabolite pools of both partners of a model cnidarian-dinoflagellate symbiosis (Aiptasia-Symbiodinium) during exposure to thermal stress via the stable isotope tracer ( C bicarbonate), coupled to GC-MS. Symbiont photodamage and pronounced bleaching coincided with substantial increases in the turnover of non C-labelled pools in the dinoflagellate (lipid and starch store catabolism). However, C enrichment of multiple compounds associated with ongoing carbon fixation and de novo biosynthesis pathways was maintained (glucose, fatty acid and lipogenesis intermediates). Minimal change was also observed in host pools of C-enriched glucose (a major symbiont-derived mobile product). However, host pathways downstream showed altered carbon fate and/or pool composition, with accumulation of compatible solutes and nonenzymic antioxidant precursors. In hospite symbionts continue to provide mobile products to the host, but at a significant cost to themselves, necessitating the mobilization of energy stores. These data highlight the need to further elucidate the role of metabolic interactions between symbiotic partners, during the process of thermal acclimation and coral bleaching.

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“…Organic acids and sugars were quantified using the GC-MS method published in Dias et al (2015). Amino acids and amines were quantified using LC-MS as described in Boughton et al (2011).…”

Section: Methodsmentioning

confidence: 99%

A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress

et al. 2017

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As integral parts of plant signaling networks, phytohormones are involved in the regulation of plant metabolism and growth under adverse environmental conditions, including salinity. Globally, salinity is one of the most severe abiotic stressors with an estimated 800 million hectares of arable land affected. Roots are the first plant organ to sense salinity in the soil, and are the initial site of sodium (Na+) exposure. However, the quantification of phytohormones in roots is challenging, as they are often present at extremely low levels compared to other plant tissues. To overcome this challenge, we developed a high-throughput LC-MS method to quantify ten endogenous phytohormones and their metabolites of diverse chemical classes in roots of barley. This method was validated in a salinity stress experiment with six barley varieties grown hydroponically with and without salinity. In addition to phytohormones, we quantified 52 polar primary metabolites, including some phytohormone precursors, using established GC-MS and LC-MS methods. Phytohormone and metabolite data were correlated with physiological measurements including biomass, plant size and chlorophyll content. Root and leaf elemental analysis was performed to determine Na+ exclusion and K+ retention ability in the studied barley varieties. We identified distinct phytohormone and metabolite signatures as a response to salinity stress in different barley varieties. Abscisic acid increased in the roots of all varieties under salinity stress, and elevated root salicylic acid levels were associated with an increase in leaf chlorophyll content. Furthermore, the landrace Sahara maintained better growth, had lower Na+ levels and maintained high levels of the salinity stress linked metabolite putrescine as well as the phytohormone metabolite cinnamic acid, which has been shown to increase putrescine concentrations in previous studies. This study highlights the importance of root phytohormones under salinity stress and the multi-variety analysis provides an important update to analytical methodology, and adds to the current knowledge of salinity stress responses in plants at the molecular level.

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Quantitative profiling of polar primary metabolites of two chickpea cultivars with contrasting responses to salinity

Cited by 88 publications

References 22 publications

Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

Mapping carbon fate during bleaching in a model cnidarian symbiosis: the application of ¹³C metabolomics

A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress

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Quantitative profiling of polar primary metabolites of two chickpea cultivars with contrasting responses to salinity

Cited by 88 publications

References 22 publications

Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

Mapping carbon fate during bleaching in a model cnidarian symbiosis: the application of 13C metabolomics

A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress

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Product

Resources

About

Mapping carbon fate during bleaching in a model cnidarian symbiosis: the application of ¹³C metabolomics