Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases

Pereira, Cátia D.; Espadas, Guadalupe; Martins, Filipa; Bertrand, Anne T.; Servais, Laurent; Sabidó, Eduard; Chevalier, Philippe; da Cruz e Silva, Odete A.B.; Rebelo, Sandra

doi:10.1016/j.bbrep.2024.101757

Biochemistry and Biophysics Reports

2024

DOI: 10.1016/j.bbrep.2024.101757

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Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases

Cátia D. Pereira,

Guadalupe Espadas,

Filipa Martins

et al.

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LAP1 Interactome Profiling Provides New Insights into LAP1’s Physiological Functions

Pereira,

Espadas,

Martins

et al. 2024

IJMS

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The nuclear envelope (NE), a protective membrane bordering the nucleus, is composed of highly specialized proteins that are indispensable for normal cellular activity. Lamina-associated polypeptide 1 (LAP1) is a NE protein whose functions are just beginning to be unveiled. The fact that mutations causing LAP1 deficiency are extremely rare and pathogenic is indicative of its paramount importance to preserving human health, anticipating that LAP1 might have a multifaceted role in the cell. Mapping the LAP1 protein interactome is, thus, imperative to achieve an integrated view of its potential biological properties. To this end, we employed in silico- and mass spectrometry-based approaches to identify candidate LAP1-interacting proteins, whose functional attributes were subsequently characterized using bioinformatics tools. Our results reveal the complex and multifunctional network of protein–protein interactions associated to LAP1, evidencing a strong interconnection between LAP1 and cellular processes as diverse as chromatin and cytoskeleton organization, DNA repair, RNA processing and translation, as well as protein biogenesis and turnover, among others. Novel interactions between LAP1 and DNA repair proteins were additionally validated, strengthening the previously proposed involvement of LAP1 in the maintenance of genomic stability. Overall, this study reaffirms the biological relevance of LAP1 and the need to deepen our knowledge about this NE protein, providing new insights about its potential functional partners that will help guiding future research towards a mechanistic understanding of LAP1’s functioning.

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LAP1 Interactome Profiling Provides New Insights into LAP1’s Physiological Functions

Pereira,

Espadas,

Martins

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

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Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases

Cited by 1 publication

References 125 publications

LAP1 Interactome Profiling Provides New Insights into LAP1’s Physiological Functions

LAP1 Interactome Profiling Provides New Insights into LAP1’s Physiological Functions

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