Quantitative proteomes and in vivo secretomes of progressive and regressive UV‐induced fibrosarcoma tumor cells: Mimicking tumor microenvironment using a dermis‐based cell‐trapped system linked to tissue chamber

Abstract

The alterations of tumor proteome and/or in vivo secretome created by host-tumor cell interaction may be crucial factors for tumors to undergo progression or regression in a host system. Two UV-induced fibrosarcoma tumor cell lines (UV-2237 progressive cells and UV-2240 regressive cells) were used as models to address this issue. Hundreds of proteins including in vivo secretome have been identified and quantified via an isotope-coded protein label (ICPL) in conjunction with high-throughput NanoLC-LTQ MS analysis. A newly designed technology using a dermis-based cell-trapped system was employed to encapsulate and grow 3-D tumor cells. A tissue chamber inserted with a tumor cell-trapped dermis was implanted into mice to mimic the tumor microenvironment. The in vivo secretome created by host-tumor interaction was characterized from samples collected from tissue chamber fluids via ICPL labeling mass spectrometric analysis. Twenty-five proteins including 14-3-3 proteins, heat shock proteins, profilin-1, and a fragment of complement C3 with differential expression in proteomes of UV-2237 and UV-2240 cells were revealed. Three secreted proteins including myeloperoxidase, alpha-2-macroglobulin, and a vitamin D-binding protein have different abundances in the in vivo secretome in response to UV-2237 and UV-2240 cells. Differential tumor proteomes and in vivo secretome were thus accentuated as potential therapeutic targets to control tumor growth.

FSHD Myotubes with Different Phenotypes Exhibit Distinct Proteomes

“…The false-positive rates, determined using a Decoy database as described [53] are as follows: 0.65% (dFSHD12_NE), 0.77% (dFSHD12_TE), 0.13% (aFSHD3_NE) and 0.81% (aFSHD3_TE). Proteins changes were considered significant when the fold change was greater than 1.5 or less than 0.7 as described in [54], [55]. At the individual level, fold changes were assessed using Student’s t -test ( t -test column in tables: *).…”

Staphylococcus aureus Hijacks a Skin Commensal to Intensify Its Virulence: Immunization Targeting β-Hemolysin and CAMP Factor

“…In-gel digestion with trypsin and protein identification via a Nano liquid chromatography linear trap quadrupole tandem mass spectrometry analysis were performed as described previously (Martin and Clynes, 1993; Shi et al ., 2007). SEQUEST was searched with a fragment ion mass tolerance of 0.5Da and a parent ion tolerance of 1.0Da.…”

Porphyrin Metabolisms in Human Skin Commensal Propionibacterium acnes Bacteria: Potential Application to Monitor Human Radiation Risk

“…Nearly everyone hosts P. acnes [41, 42], which accounts for approximately half of the total skin microbiome [44–46]. In addition, the commensal bacterium was found at a very high percentage on the foreheads [10 3 to 10 7 colony-forming unit (CFU)] of normal individuals in Seattle (92%) and Alaska (81%) [47, 48], suggesting no differences of bacterial colonization between different populations during various seasons. Besides porphyrins that are highlighted in this review, there are many fluorophores in P. acnes that potentially can be used to reflect the radiation damage.…”