Quantitative proteomic analyses provide insights into the hyalinocytes and granulocytes phagocytic killing of ivory shell Babylonia areolata in vitro

Di, Guilan; Zhu, Guorong; Chen, Xinhua; Miao, Xiulian; Li, Min; Fu, Jun; Wang, Ning; Zhu, Lei; Huang, Meng; Ke, Caihuan

doi:10.1016/j.aquaculture.2021.736898

Cited by 6 publications

(3 citation statements)

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“…Initially, the distinction between hyalinocytes and granulocytes was made based on morphological parameters, years later it has been confirmed that there are also functional differences between both haemolymph cell types after analysis of metabolic and functional parameters (122,221). Recently, research efforts are focusing on revealing dissimilar immune functions among marine invertebrate haemocyte subpopulations, for example in ascidians (222); crustaceans as shrimps (223)(224)(225)(226)(227)(228), lobsters (229) and crabs (230); gastropod molluscs as sea snails (231)(232)(233)(234), abalones (235) and sea hares (236); and bivalve molluscs as oysters (182,216,237,238). This review focuses on the major findings in functional differences between the main bivalve haemocyte types.…”

Section: Functional Differences Between Hyalinocytes and Granulocytesmentioning

confidence: 99%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

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Bivalve molluscs stand out for their ecological success and their key role in the functioning of aquatic ecosystems, while also constituting a very valuable commercial resource. Both ecological success and production of bivalves depend on their effective immune defence function, in which haemocytes play a central role acting as both the undertaker of the cellular immunity and supplier of the humoral immunity. Bivalves have different types of haemocytes, which perform different functions. Hence, identification of cell subpopulations and their functional characterisation in immune responses is essential to fully understand the immune system in bivalves. Nowadays, there is not a unified nomenclature that applies to all bivalves. Characterisation of bivalve haemocyte subpopulations is often combined with 1) other multiple parameter assays to determine differences between cell types in immune-related physiological activities, such as phagocytosis, oxidative stress and apoptosis; and 2) immune response to different stressors such as pathogens, temperature, acidification and pollution. This review summarises the major and most recent findings in classification and functional characterisation of the main haemocyte types of bivalve molluscs.

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Section: Functional Differences Between Hyalinocytes and Granulocytesmentioning

confidence: 99%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

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“…The Oxidative phosphorylation pathway related to energy metabolism was enriched in HCs of all treatment groups. This pathway might provide oxidative energy and kill pathogens by oxidative burst for HCs 74,75 . Granulocytes, especially GRCs, were mainly enriched in immune-related pathways.…”

Section: Discussionmentioning

confidence: 99%

Single-Cell Transcriptomic Analysis of Specific Responses of Different Cell Populations of Hemocytes to the Re-infection of Bacteria, a Case Study in Abalone

Zhang,

Sun

et al. 2024

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It is commonly believed that invertebrates lack immune memory due to the absence of immunoglobulins, related molecules, cells, and organs. However, our previous research demonstrated that Haliotis discus hannai, a prominent abalone species cultivated in China, often faces substantial economic losses due to diseases, particularly those caused by Vibrio sp. exhibited higher survival rates upon re-infection with Vibrio parahaemolyticus compared to the initial infection, implying the existence of immune memory. We hypothesized that hemocytes, which play a critical role in pathogen resistance in abalone, might be involved in the immune memory process. Therefore, we aimed to investigate the hemocyte response mechanism to V. parahaemolyticus re-infection to provide valuable insights for preventing and controlling abalone vibriosis and advancing sustainable abalone aquaculture. Additionally, our research aimed to contribute to understanding the origin and evolution of immune memory mechanisms. This study constructed a transcriptome map of abalone hemocytes using 10× Genomics single-cell RNA sequencing (scRNA-seq). Traditionally, abalone hemocytes were categorized into three cell types: hyalinocytes, semi-granulocytes, and granulocytes. The initial cell division resulted in the formation of 15 clusters further through subsequent analysis using scRNA-seq. Among these clusters, cluster_11 exhibited unique characteristics, indicating a more mature cluster of GRCs. This specific subpopulation displayed significant functionality as a core immune regulator, manifesting robust phagocytic and endocytic activities and substantial involvement in signal transduction and immune regulatory processes. Furthermore, we analyzed and detailed functional variances among different hemocyte types. Through the implementation of RNA interference technology, we validated the interplay between key signaling pathways. Interestingly, our findings suggested the potential existence of a classical TLR/NF-κB signaling pathway in abalone hemocytes, which may contribute to the immune regulation process in response to V. parahaemolyticus re-infection, as preliminarily confirmed in our study.

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“…This seafood is rich in nutrients and has delicious flavour, making it a popular choice. It also has a large market demand [ [1] , [2] , [3] , [4] ]. Over recent years, due to breakthroughs and improvements in breeding technology, there has been a rapid development of industrial breeding of spotted babylon in the southeast coastal areas of China.…”

Section: Introductionmentioning

confidence: 99%