Quantitative proteomic analysis comparing grades ICRS1 and ICRS3 in patients with osteoarthritis

Abstract

Osteoarthritis (OA), which is caused by joint damage, is the most common form of arthritis, affecting millions of people worldwide. This damage can accumulate over time, which is why aging is one of the main contributors to joint damage associated with OA. The OA-related proteins that have been reported to date have been identified by the comparative analysis of OA patients with normal controls, following surgical or pharmacological treatment. For the first time, the present study analyzed OA-related proteins in patients with OA according to the International Cartilage Repair Society (ICRS) scale. Changes in protein expression can be observed during the OA process. The present study demonstrated differential protein expression patterns in articular cartilage from ICRS1and ICRS3-graded OA patients. ICRS grade-matched OA knee samples from 12 OA patients, 6 ICRS grade 1 patients and 6 ICRS3 patients were subjected to proteomic analysis using the LTQ-Orbitrap mass spectrometry system. A total of 231 unique proteins were identified as expressed across the ICRS1 and ICRS3 OA patient groups. Relative differences in protein expression associated with the following classifications were observed: Biological adhesion, cell killing, cellular process, development process and molecular function. Although some of these proteins have been previously reported to be associated with rheumatoid arthritis, including cartilage oligomeric matrix protein, collagen types, angiogenin, complement C5 and CD59 glycoprotein, numerous additional proteins were newly identified, which may further help our understanding of disease pathogenesis. These findings suggested that these proteins may be used to develop novel therapeutic targets for OA.

Identification and quantification of degradome components in human synovial fluid reveals an increased proteolytic activity in knee osteoarthritis patients vs controls

“…In both the qualitative and quantitative comparisons, we found peptide levels of HRG to differ the most between the OA and the control group (Table S5 and S6). HRG has been reported to be increased in OA in previous proteomics studies [27,28]. HRG is an abundant protein in plasma and has been referred to as "the Swiss army knife" of plasma due to its involvement in many biological processes and ability to interact with multiple ligands simultaneously [29].…”

Study the Level of Collagen2-1NO2 (Coll2-1NO2) in Serum of Iraqi Patients with Osteoarthritis

“…This condition causes irreparable damage to the function of the joints as a consequence of aging, trauma, wear and tear on cartilage, and persistent inflammation in the joints. An precise diagnosis of OA, which is characterized by the increasing narrowing of joint space, the formation of osteophytes, the sclerosis of the subchondral bone, and the generation of cysts, is made feasible by X-ray radiography (8) In OA, dysregulation caused by the presence of numerous bio factors results in the loss of cartilage homeostasis, which in turn causes the degradation of the collagen-and proteoglycan-rich extracellular matrix (ECM), fibrillation and erosion of the articular surface, cell death, matrix calcification, and vascular invasion (9).…”