Quantitative proteomic analysis of Down syndrome in the umbilical cord blood using iTRAQ

Sui, Weiguo; Zhang, Ruohan; Chen, Jiejing; He, Hao; Cui, Zhenzhen; Ou, Minglin; Li, Wuxian; Qi, Suwen; Wen, Jingli; Lin, Xiuhua; Dai, Yong

doi:10.3892/mmr.2014.2828

Cited by 14 publications

(13 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As iTRAQ has previously been suggested to be suitable for identifying novel plasma biomarkers (25), this method has been used to detect for potential quantitative changes in the plasma proteome of fetuses with DS, compared with normal fetuses. These proteins are found in the sera of patients with Alzheimer's disease, which has a similar pathology to DS (26).…”

Section: Discussionmentioning

confidence: 99%

Integrated microRNA and protein expression analysis reveals novel microRNA regulation of targets in fetal down syndrome

Hua

Sui

et al. 2016

Molecular Medicine Reports

Self Cite

View full text Add to dashboard Cite

Down syndrome (DS) is caused by trisomy of human chromosome 21 and is associated with a number of deleterious phenotypes. To investigate the role of microRNA (miRNA) in the regulation of DS, high-throughput Illumina sequencing technology and isobaric tagging for relative and absolute protein quantification analysis were utilized for simultaneous expression profiling of miRNA and protein in fetuses with DS and normal fetuses. A total of 344 miRNAs were associated with DS. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to investigate the proteins found to be differentially expressed. Functionally important miRNAs were determined by identifying enriched or depleted targets in the transcript and the protein expression levels were consistent with miRNA regulation. The results indicated that GRB2, TMSB10, RUVBL2, the hsa-miR-329 and hsa-miR-27b, hsa-miR-27a targets, and MAPK1, PTPN11, ACTA2 and PTK2 or other differentially expressed proteins were connected with each other directly or indirectly. Integrative analysis of miRNAs and proteins provided an expansive view of the molecular signaling pathways in DS.

show abstract

Section: Discussionmentioning

confidence: 99%

Integrated microRNA and protein expression analysis reveals novel microRNA regulation of targets in fetal down syndrome

Hua

Sui

et al. 2016

Molecular Medicine Reports

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sui et al compared umbilical cord blood from mothers carrying Down syndrome fetuses ( n = 6) and healthy fetuses ( n = 11). Using iTRAQ labeling, SCX fractionation and LC‐MALDI they identified 505 proteins and reported five putative biomarkers …”

Section: Applications Of Isobaric Labeling For Serum and Plasma Protementioning

confidence: 99%

“…Using iTRAQ labeling, SCX fractionation and LC-MALDI they identified 505 proteins and reported five putative biomarkers. 169…”

Section: Pregnancy and Gynecologymentioning

confidence: 99%

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

Moulder

Bhosale

Goodlett

et al. 2017

Mass Spectrometry Reviews

128

View full text Add to dashboard Cite

Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.

show abstract

“…Of those, 13 were found to be up-regulated in DS samples and six were down-regulated. APOE, complement factor B (CFB), amyloid P component), serum (APCS) (up-regulated), matrin-3 and osteopontin (down-regulated) were previously associated with DS (139). Interestingly, the other proteins were related to iron and copper homeostasis, blood pressure, and coagulation, all components of the metabolic syndrome, which is a known long-term consequence of IUGR in adult life (141).…”

Section: Proteomics and Art Outcomementioning

confidence: 99%