Quantitative proteomic analysis of milk fat globule membrane (MFGM) proteins from donkey colostrum and mature milk

Li, Weixuan; Li, Mohan; Cao, Xiaohong; Yang, Mei; Han, Hongjiao; Kong, Fanhua

doi:10.1039/c9fo00386j

Cited by 43 publications

(24 citation statements)

References 53 publications

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“…It has been indicated to be more suitable for children and elderly people due to its remarkable nutritional value and less allergenic. Besides, milk fat globules in DM are smaller and more easily digested and absorbed by infants ( 2 ). With its obvious advantages, the demand of direct consumption for DM has increased.…”

Section: Introductionmentioning

confidence: 99%

“…To reveal the differences in the formation of MFGM in different mammals, the MFGM proteins of cow, yak, buffalo, goat, horse, camel and human were also compared by iTRAQ proteomics ( 6 ). Recently, Li et al hoped to explore the changes in the regulation mechanism of different lactation stages by analyzing the differences of MFGM proteins between donkey colostrum and mature milk ( 2 ). However, studies of the DM MFGM proteome are relatively sparse and less comprehensive, especially fully comparative analyses of MFGM protein compositions and potential biological activities between DM and other species.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Label-Free Proteomic Analysis of Milk Fat Globule Membrane in Donkey and Human Milk

Zhang

Jiang

Ji³

et al. 2021

Front. Nutr.

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Previous studies have found donkey milk (DM) has the similar compositions with human milk (HM) and could be used as a potential hypoallergenic replacement diet for babies suffering from cow's milk allergy. Milk fat globule membrane (MFGM) proteins are involved in many biological functions, behaving as important indicators of the nutritional quality of milk. In this study, we used label-free proteomics to quantify the differentially expressed MFGM proteins (DEP) between DM (in 4–5 months of lactation) and HM (in 6–8 months of lactation). In total, 293 DEP were found in these two groups. Gene Ontology (GO) enrichment analysis revealed that the majority of DEP participated in regulation of immune system process, membrane invagination and lymphocyte activation. Several significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were determined for the DEP, such as lysosome, galactose metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. Our study may provide valuable information in the composition of MFGM proteins in DM and HM, and expand our knowledge of different biological functions between DM and HM.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Label-Free Proteomic Analysis of Milk Fat Globule Membrane in Donkey and Human Milk

Zhang

Jiang

Ji³

et al. 2021

Front. Nutr.

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“…Milk fat globule membrane (MFGM) secreted from the apical membrane carries a part of the cytoplasmic fraction of MECs, and they also represent partial information on the proteome of terminally differentiated MECs 23 . Studies on MFGM proteome reported a total of 632, 137, 947 and 1104 proteins in cattle, goat, donkey, and human respectively 24,25 . On the other hand, a proteomics study on mice mammary tissue suggested the biological significance of RNA polymerase B transcription factor 3 (BTF3) in the differentiation of MECs during pregnancy 26 .…”

Section: Discussionmentioning

confidence: 99%

In-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation

Jaswal

Anand

Kumar

et al. 2020

Sci Rep

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The mature mammary gland is made up of a network of ducts that terminates in alveoli. The innermost layer of alveoli is surrounded by the differentiated mammary epithelial cells (MECs), which are responsible for milk synthesis and secretion during lactation. However, the MECs are in a state of active proliferation during pregnancy, when they give rise to network like structures in the mammary gland. Buffalo (Bubalus bubalis) constitute a major source of milk for human consumption, and the MECs are the major precursor cells which are mainly responsible for their lactation potential. The proteome of MECs defines their functional state and suggests their role in various cellular activities such as proliferation and lactation. To date, the proteome profile of MECs from buffalo origin is not available. In the present study, we have profiled in-depth proteome of in vitro cultured buffalo MECs (BuMECs) during active proliferation using high throughput tandem mass spectrometry (MS). MS analysis identified a total of 8330, 5970, 5289, 4818 proteins in four sub-cellular fractions (SCFs) that included cytosolic (SCF-I), membranous and membranous organelle's (SCF-II), nuclear (SCF-III), and cytoskeletal (SCF-IV). However, 792 proteins were identified in the conditioned media, which represented the secretome. Altogether, combined analysis of all the five fractions (SCFs-I to IV, and secretome) revealed a total of 12,609 non-redundant proteins. The KEGG analysis suggested that these proteins were associated with 325 molecular pathways. Some of the highly enriched molecular pathways observed were metabolic, MAPK, PI3-AKT, insulin, estrogen, and cGMP-PKG signalling pathway. The newly identified proteins in this study are reported to be involved in NOTCH signalling, transport and secretion processes.The mammary gland, a unique organ of mammals, is a derivative of ventral skin 1 and has evolved to provide nutrition and immune protection to the offspring. Though the anatomical and physiological features of a mammary gland are diverse, the basic structure is similar in all species. The mature mammary gland is constituted by a network of tubular-alveolar structures, with their lumen lined by a layer of polarized secretory mammary epithelial cells (MECs). The MECs are dynamic components of the mammary gland, which undergo repeated rounds of proliferation and differentiation during different developmental stages such as pregnancy, and lactation 2 . The quantity and functional differentiation of MECs in the udder determine the milk-producing ability in livestock. Bovine (ruminants) are physiologically distinct from other mammals such as humans, mice and rats. The present study on in-depth proteome analysis of buffalo (Bubalus bubalis) MECs (BuMECs) advances our knowledge on the protein machinery operating in mammary gland development and lactogenesis in buffalo, which can be extrapolated in other mammalian species as well. Among the domestic livestock species, cattle (Bos taurus) and

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“…This important information helped identify the main proteins with immunity-promoting properties for newborns [66]. These analyses have been performed to study and compare the proteome of various MFGMs, mainly from humans and cows [64][65][66], but also from other species such as the goat, yak, buffalo, horse, and donkey [61,62,[67][68][69][70].…”

Section: Comparison Of Mfgm Proteome Between Different Species and Lamentioning

confidence: 99%

“…These analyses have been performed to study and compare the proteome of various MFGMs, mainly from humans and cows [ 64 , 65 , 66 ], but also from other species such as the goat, yak, buffalo, horse, and donkey [ 61 , 62 , 67 , 68 , 69 , 70 ].…”

Section: Comparison Of Mfgm Proteome Between Different Species Andmentioning

confidence: 99%

Comparative Proteomics of Milk Fat Globule Membrane (MFGM) Proteome across Species and Lactation Stages and the Potentials of MFGM Fractions in Infant Formula Preparation

Manoni

Lorenzo

Ottoboni

et al. 2020

Foods

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Milk is a lipid-in-water emulsion with a primary role in the nutrition of newborns. Milk fat globules (MFGs) are a mixture of proteins and lipids with nutraceutical properties related to the milk fat globule membrane (MFGM), which protects them, thus preventing their coalescence. Human and bovine MFGM proteomes have been extensively characterized in terms of their formation, maturation, and composition. Here, we review the most recent comparative proteomic analyses of MFGM proteome, above all from humans and bovines, but also from other species. The major MFGM proteins are found in all the MFGM proteomes of the different species, although there are variations in protein expression levels and molecular functions across species and lactation stages. Given the similarities between the human and bovine MFGM and the bioactive properties of MFGM components, several attempts have been made to supplement infant formulas (IFs), mainly with polar lipid fractions of bovine MFGM and to a lesser extent with protein fractions. The aim is thus to narrow the gap between human breast milk and cow-based IFs. Despite the few attempts made to date, supplementation with MFGM proteins seems promising as MFGM lipid supplementation. A deeper understanding of MFGM proteomes should lead to better results.

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Quantitative proteomic analysis of milk fat globule membrane (MFGM) proteins from donkey colostrum and mature milk

Abstract: The composition and functions of milk fat globule membrane (MFGM) proteins are important indicators of the nutritional quality of milk.

Cited by 43 publications

References 53 publications

Quantitative Label-Free Proteomic Analysis of Milk Fat Globule Membrane in Donkey and Human Milk

Quantitative Label-Free Proteomic Analysis of Milk Fat Globule Membrane in Donkey and Human Milk

In-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation

Comparative Proteomics of Milk Fat Globule Membrane (MFGM) Proteome across Species and Lactation Stages and the Potentials of MFGM Fractions in Infant Formula Preparation

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