Quantitative Proteomic Analysis Reveals Important Roles of the Acetylation of ER-Resident Molecular Chaperones for Conidiation in Fusarium oxysporum

Lv, Fangjiao; Xu, Yang; Gabriel, Dean W.; Wang, Xue; Zhang, Ning; Liang, Wenxing

doi:10.1016/j.mcpro.2022.100231

Cited by 5 publications

(2 citation statements)

References 57 publications

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“…The mycelial radial growth was measured after 3 d of incubation. For conidiation, conidia with a concentration of 10 3 conidia ml −1 of culture volume were inoculated into one‐tenth of the standard concentration of YEPD and incubated at 25°C, 150 rpm for 48 h (Lv et al ., 2022). For conidial germination assay, fresh conidia of strains were harvested and adjusted to the concentration of 2.5 × 10 5 conidia ml −1 in PDB (liquid PDA).…”

Section: Methodsmentioning

confidence: 99%

Sir2‐mediated cytoplasmic deacetylation facilitates pathogenic fungi infection in host plants

Zhang,

Hu,

Liu

et al. 2023

New Phytologist

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Summary Lysine acetylation is an evolutionarily conserved and widespread post‐translational modification implicated in the regulation of multiple metabolic processes, but its function remains largely unknown in plant pathogenic fungi. A comprehensive analysis combined with proteomic, molecular and cellular approaches was presented to explore the roles of cytoplasmic acetylation in Fusarium oxsysporum f.sp. lycopersici (Fol). The divergent cytoplasmic deacetylase FolSir2 was biochemically characterized, which is contributing to fungal virulence. Based on this, a total of 1752 acetylated sites in 897 proteins were identified in Fol via LC–MS/MS analysis. Further analyses of the quantitative acetylome revealed that 115 proteins representing two major pathways, translational and ribosome biogenesis, were hyperacetylated in the ∆Folsir2 strain. We experimentally examined the regulatory roles of FolSir2 on K271 deacetylation of FolGsk3, a serine/tyrosine kinase implicated in a variety of cellular functions, which was found to be crucial for the activation of FolGsk3 and thus modulated Fol pathogenicity. Cytoplasmic deacetylation by FolSir2 homologues has a similar function in Botrytis cinerea and likely other fungal pathogens. These findings reveal a conserved mechanism of silent information regulator 2‐mediated cytoplasmic deacetylation that is involved in plant‐fungal pathogenicity, providing a candidate target for designing broad‐spectrum fungicides to control plant diseases.

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Section: Methodsmentioning

confidence: 99%

Sir2‐mediated cytoplasmic deacetylation facilitates pathogenic fungi infection in host plants

Zhang,

Hu,

Liu

et al. 2023

New Phytologist

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“…However, the exploration of lysine acetylation in F. oxysporum has been limited to secreted proteins and the process of conidiation in F. oxysporum f. sp. lycoperisici [ 19 , 20 ]. The overall profile of lysine acetylation of F. oxysporum is required for a comprehensive understanding of pathogenesis and virulence evolution.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated Fusarium oxysporum f. sp. cucumerinum Isolates during Cucumber Colonization

Zhou,

Lu,

Hao

et al. 2023

JoF

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Fusarium oxysporum f. sp. cucumerinum (Foc) is a prominent pathogen that adversely affects cucumber (Cucumis sativus) production. In the pathogen’s parasitic lifestyle, the pathogenesis and virulence evolution may be regulated by lysine acetylation, as demonstrated in many living organisms. However, its specific function in Foc remains poorly understood. In this study, the acetylome profiles of a mild virulence strain (foc-3b) and its derived virulence-enhanced strain (Ra-4) were analyzed before and post-inoculation on cucumber plants. In total, 10,664 acetylation sites were identified corresponding to 3874 proteins, and 45 conserved acetylation motifs were detected. Through comparison of the acetylomes, numerous differentially lysine-acetylated proteins were enriched in energy metabolism and protein processing processes, indicating the critical role of lysine acetylation during the transition from the saprotrophic lifestyle to the parasitic lifestyle. Comparative acetylome analyses on the two virulence-differentiated strains revealed that several differentially lysine-acetylated proteins were involved in pathways of defense response and energy metabolism. Ra-4 showed enhanced energy metabolism compared to foc-3b. This indicates that robust metabolic activity is required to achieve high virulence and facilitating adaptive evolution. Additionally, faster host responses are supported by an ample energy supply enhancing virulence. Thus, lysine acetylation plays a crucial role in the pathogenesis and virulence evolution of Foc.

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Fungal heat shock proteins: molecular phylogenetic insights into the host takeover

Sagini,

Ligabue-Braun

2024

Sci Nat

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Quantitative Proteomic Analysis Reveals Important Roles of the Acetylation of ER-Resident Molecular Chaperones for Conidiation in Fusarium oxysporum

Cited by 5 publications

References 57 publications

Sir2‐mediated cytoplasmic deacetylation facilitates pathogenic fungi infection in host plants

Sir2‐mediated cytoplasmic deacetylation facilitates pathogenic fungi infection in host plants

Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated Fusarium oxysporum f. sp. cucumerinum Isolates during Cucumber Colonization

Fungal heat shock proteins: molecular phylogenetic insights into the host takeover

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