Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer

Wang, Guoqiang; Yang, Qiongzhi; Li, Maoyu; Zhang, Ye; Cai, Yuxiang; Liang, Xujun; Fu, Ying; Xiao, Zhefeng; Zhou, Minze; Xie, Zheng; Huang, Huichao; Huang, Ya‐Hui; Chen, Yongheng; He, Qiaojun; Peng, Fang; Chen, Zhuchu

doi:10.1101/561555

Cited by 2 publications

(1 citation statement)

References 59 publications

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“…Despite its prevalence, the significance and underlying mechanisms of EPHA2 remain unclear. However, increasing evidence suggests that the expression of human EPHA2 is notably high in various cancer types, such as prostate, lung, colorectal, cervical, ovarian, breast, and skin cancers [11,[14][15][16] . The expression of EPHA2 is correlated with an undesirable prognosis, increased metastatic capacity, and decreased survival rates in cancer patients [17] .…”

Section: Introductionmentioning

confidence: 99%

Molecular Characteristics and Prognostic Role of EPHA2 in Human Tumors via Pan-cancer Analysis

Wang,

Liang,

Guo

et al. 2023

Preprint

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Background: The tyrosine kinase ephrin type-A receptor 2（EPHA2）is remarkably elevated expressed in various tumors and plays a crucial role in cancer tumorigenesis and progression, while pan-cancer analyses (PCA) are currently lacking. This study was designed to analyze the expression status and prognostic significance of EPHA2 in pan-cancer. Methods: By mining The Cancer Genome Atlas (TCGA) data, we performed a comprehensive and systematic characterization of EPHA2 across more than 10,000 samples of 33 types of cancer. Results: EPHA2 expressions were substantially different in most of the normal control and tumor tissues and considerably associated with the prognosis of tumor patients. In addition, the missense mutation is more prevalent in EPHA2 genetic modification in malignancies. Moreover, a notable association has been developed between EPHA2 expressions and cancer-associated fibroblasts (CAFs) in most TCGA cancers. Furthermore, Functional enrichment analysis showed that the biological role of EPHA2 in tumors is mainly involved in some noticeably pro-oncogenic pathways, such as the Ras signaling pathway, PI3K-Akt signaling pathway, ErbB signaling pathway, MAPK signaling pathway, etc. Conclusions: This study provides the first PCA with the extensive role of EPHA2 in various tumors, and EPHA2 is potentially involved in many cancer types and can be developed as candidates for cancer diagnosis, prognosis, and therapeutic biomarkers.

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Section: Introductionmentioning

confidence: 99%

Molecular Characteristics and Prognostic Role of EPHA2 in Human Tumors via Pan-cancer Analysis

Wang,

Liang,

Guo

et al. 2023

Preprint

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Subendothelial stiffness alters endothelial cell traction force generation while exerting a minimal effect on the transcriptome

Bastounis

Yeh

Theriot

2019

Sci Rep

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Endothelial cells respond to changes in subendothelial stiffness by altering their migration and mechanics, but whether those responses are due to transcriptional reprogramming remains largely unknown. We measured traction force generation and also performed gene expression profiling for two endothelial cell types grown in monolayers on soft or stiff matrices: primary human umbilical vein endothelial cells (HUVEC) and immortalized human microvascular endothelial cells (HMEC-1). Both cell types respond to changes in subendothelial stiffness by increasing the traction stresses they exert on stiffer as compared to softer matrices, and exhibit a range of altered protein phosphorylation or protein conformational changes previously implicated in mechanotransduction. However, the transcriptome has only a minimal role in this conserved biomechanical response. Only few genes were differentially expressed in each cell type in a stiffness-dependent manner, and none were shared between them. In contrast, thousands of genes were differentially regulated in HUVEC as compared to HMEC-1. HUVEC (but not HMEC-1) upregulate expression of TGF-β2 on stiffer matrices, and also respond to application of exogenous TGF-β2 by enhancing their endogenous TGF-β2 expression and their cell-matrix traction stresses. Altogether, these findings provide insights into the relationship between subendothelial stiffness, endothelial mechanics and variation of the endothelial cell transcriptome, and reveal that subendothelial stiffness, while critically altering endothelial cells’ mechanical behavior, minimally affects their transcriptome.

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Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer

Cited by 2 publications

References 59 publications

Molecular Characteristics and Prognostic Role of EPHA2 in Human Tumors via Pan-cancer Analysis

Molecular Characteristics and Prognostic Role of EPHA2 in Human Tumors via Pan-cancer Analysis

Subendothelial stiffness alters endothelial cell traction force generation while exerting a minimal effect on the transcriptome

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