Quantitative proteomics analysis by iTRAQ revealed underlying changes in thermotolerance of Arthrospira platensis

Chang, Rong; Lv, Bingxin; Li, Bosheng

doi:10.1016/j.jprot.2017.06.015

Cited by 17 publications

(7 citation statements)

References 44 publications

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“…Such stress tolerance may be related to the generation of reactive oxygen species (ROS). Microbial cells generate a large amount of ROS, especially when grown at growth-limiting high temperatures ( Davidson et al, 1996 ; Matsushita et al, 2016 ; Chang et al, 2017 ; Nantapong et al, 2019 ), and thermally adapted strains have been shown to generate less ROS than the wild-type strain when grown at higher growth temperatures ( Matsumoto et al, 2018 , 2020 ). Acetic acid fermentation may similarly generate ROS due to structural damage to the cell membrane caused by acetic acid and/or ethanol.…”

Section: Resultsmentioning

confidence: 99%

Mutations in degP and spoT Genes Mediate Response to Fermentation Stress in Thermally Adapted Strains of Acetic Acid Bacterium Komagataeibacter medellinensis NBRC 3288

et al. 2022

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An acetic acid bacterium, Komagataeibacter medellinensis NBRC 3288, was adapted to higher growth temperatures through an experimental evolution approach in acetic acid fermentation conditions, in which the cells grew under high concentrations of ethanol and acetic acid. The thermally adapted strains were shown to exhibit significantly increased growth and fermentation ability, compared to the wild strain, at higher temperatures. Although the wild cells were largely elongated and exhibited a rough cell surface, the adapted strains repressed the elongation and exhibited a smaller cell size and a smoother cell surface than the wild strain. Among the adapted strains, the ITO-1 strain isolated during the initial rounds of adaptation was shown to have three indel mutations in the genes gyrB, degP, and spoT. Among these, two dispensable genes, degP and spoT, were further examined in this study. Rough cell surface morphology related to degP mutation suggested that membrane vesicle-like structures were increased on the cell surface of the wild-type strain but repressed in the ITO-1 strain under high-temperature acetic acid fermentation conditions. The ΔdegP strain could not grow at higher temperatures and accumulated a large amount of membrane vesicles in the culture supernatant when grown even at 30°C, suggesting that the degP mutation is involved in cell surface stability. As the spoT gene of ITO-1 lost a 3′-end of 424 bp, which includes one (Act-4) of the possible two regulatory domains (TGS and Act-4), two spoT mutant strains were created: one (ΔTGSAct) with a drug cassette in between the 5′-half catalytic domain and 3′-half regulatory domains of the gene, and the other (ΔAct-4) in between TGS and Act-4 domains of the regulatory domain. These spoT mutants exhibited different growth responses; ΔTGSAct grew better in both the fermentation and non-fermentation conditions, whereas ΔAct-4 did only under fermentation conditions, such as ITO-1 at higher temperatures. We suggest that cell elongation and/or cell size are largely related to these spoT mutations, which may be involved in fermentation stress and thermotolerance.

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Section: Resultsmentioning

confidence: 99%

Mutations in degP and spoT Genes Mediate Response to Fermentation Stress in Thermally Adapted Strains of Acetic Acid Bacterium Komagataeibacter medellinensis NBRC 3288

et al. 2022

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“…Such stress tolerance may be related to the generation of reactive oxygen species (ROS). Microbial cells generate a large amount of ROS, especially when grown at growth-limiting high temperatures Matsushita et al, 2016;Chang et al, 2017;Nantapong et al, 2019), and thermally adapted strains have been shown to generate less ROS than the wild-type strain when grown at higher growth temperatures (Matsumoto et al, 2018.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Microbial Stress: From Model Organisms to Applications in Food, Microbiotechnology and Medicine

2022

Frontiers Research Topics

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Frontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation.

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“…Such an increased NADPH level, derived from increased metabolic flux, was expected to contribute to protection from thermal stress. As described in the introduction, although the level of ROS generation is increased by several stressors, including thermal stress (14)(15)(16)(17), it is expected to be lowered in stress-resistant strains, where ROS-scavenging activity is often high (16,26). Because some ROS-scavenging enzymes require NADPH (23,24), the increase in the intracellular NADPH concentration may support the stress resistance of cells (25).…”

Section: Figmentioning

confidence: 99%

“…Aerobic organisms generate reactive oxygen species (ROS) causing cell damage, which has been shown to increase when microbes are grown at a lethal high temperature (14,15) or after heat treatment in plants (16,17). ROS are generated in the respiratory chain, mainly by flavoproteins, especially NADH dehydrogenase, as demonstrated in many microbes, such as yeast (18), E. coli (19,20), and Corynebacterium glutamicum (21,22).…”

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confidence: 99%

A Single-Nucleotide Insertion in a Drug Transporter Gene Induces a Thermotolerance Phenotype in Gluconobacter frateurii by Increasing the NADPH/NADP ⁺ Ratio via Metabolic Change

Matsumoto

Hattori

Matsutani

et al. 2018

Appl Environ Microbiol

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Thermotolerant microorganisms are beneficial to the fermentation industry because they reduce the need for cooling and offer other operational advantages. Previously, we obtained a thermally adapted strain by experimental evolution. In the present study, we found only a single G insertion in the adapted strain, which causes a frameshift in a gene encoding a putative drug transporter. A mutant derivative strain with the single G insertion in the transporter gene (Wild-G) was constructed from the wild-type strain and showed increased thermotolerance. We found that the thermotolerant strains accumulated substantial intracellular trehalose and manifested a defect in sorbose assimilation, suggesting that the transporter is partly involved in trehalose efflux and sorbose uptake and that the defect in the transporter can improve thermotolerance. The Δ strain, constructed by elimination of the trehalose synthesis gene in the wild type, showed no trehalose production but, unexpectedly, much better growth than the adapted strain at high temperatures. The Δ mutant produced more acetate as the final metabolite than the wild-type strain did. We hypothesized that trehalose does not contribute to thermotolerance directly; rather, a metabolic change including increased carbon flux to the pentose phosphate pathway may be the key factor. The NADPH/NADP ratio was higher in strain Wild-G, and much higher in the Δ strain, than in the wild-type strain. Levels of reactive oxygen species (ROS) were lower in the thermotolerant strains. We propose that the defect of the transporter causes the metabolic flux to generate more NADPH, which may enhance thermotolerance in The biorefinery industry has to ensure that microorganisms are robust and retain their viability and function at high temperatures. Here we show that, an industrially important member of the acetic acid bacteria, exhibited enhanced thermotolerance through the reduction of trehalose excretion after thermal adaptation. Although intracellular trehalose may play a key role in thermotolerance, the molecular mechanisms of action of trehalose in thermotolerance are a matter of debate. Our mutated strain that was defective in trehalose synthase genes, producing no trehalose but a larger amount of acetic acid as the end metabolite instead, unexpectedly showed higher thermotolerance than the wild type. Our adapted and mutated thermotolerant strains showed increased NADPH/NADP ratios and reductions in ROS levels. We concluded that in , trehalose does not contribute to thermotolerance directly; rather, the metabolic change increases the NADPH/NADP ratio to enhance thermotolerance.

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Quantitative proteomics analysis by iTRAQ revealed underlying changes in thermotolerance of Arthrospira platensis

Cited by 17 publications

References 44 publications

Mutations in degP and spoT Genes Mediate Response to Fermentation Stress in Thermally Adapted Strains of Acetic Acid Bacterium Komagataeibacter medellinensis NBRC 3288

Mutations in degP and spoT Genes Mediate Response to Fermentation Stress in Thermally Adapted Strains of Acetic Acid Bacterium Komagataeibacter medellinensis NBRC 3288

Microbial Stress: From Model Organisms to Applications in Food, Microbiotechnology and Medicine

A Single-Nucleotide Insertion in a Drug Transporter Gene Induces a Thermotolerance Phenotype in Gluconobacter frateurii by Increasing the NADPH/NADP ⁺ Ratio via Metabolic Change

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Quantitative proteomics analysis by iTRAQ revealed underlying changes in thermotolerance of Arthrospira platensis

Cited by 17 publications

References 44 publications

Mutations in degP and spoT Genes Mediate Response to Fermentation Stress in Thermally Adapted Strains of Acetic Acid Bacterium Komagataeibacter medellinensis NBRC 3288

Mutations in degP and spoT Genes Mediate Response to Fermentation Stress in Thermally Adapted Strains of Acetic Acid Bacterium Komagataeibacter medellinensis NBRC 3288

Microbial Stress: From Model Organisms to Applications in Food, Microbiotechnology and Medicine

A Single-Nucleotide Insertion in a Drug Transporter Gene Induces a Thermotolerance Phenotype in Gluconobacter frateurii by Increasing the NADPH/NADP + Ratio via Metabolic Change

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A Single-Nucleotide Insertion in a Drug Transporter Gene Induces a Thermotolerance Phenotype in Gluconobacter frateurii by Increasing the NADPH/NADP ⁺ Ratio via Metabolic Change