Quantitative Proteomics Analysis of Berberine-Treated Colon Cancer Cells Reveals Potential Therapy Targets

Li, Pengfei; Zou, Hao; Liu, Huanhuan; Zhu, Bojing; Dang, Liuyi; Ma, Chen; Xu, Yintai; Zhang, Yiyan; Fan, Daidi; Sun, Shisheng

doi:10.3390/biology10030250

Cited by 13 publications

(14 citation statements)

References 72 publications

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“…The details of sample treatment were described previously ( 18 ). Treated and untreated cells were washed three times by ice-cold PBS buffer to remove the cell culture medium.…”

Section: Methodsmentioning

confidence: 99%

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

Zou

et al. 2021

Front. Immunol.

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Macrophages can be polarized into classically activated macrophages (M1) and alternatively activated macrophages (M2) in the immune system, performing pro-inflammatory and anti-inflammatory functions, respectively. Human THP-1 and mouse RAW264.7 cell line models have been widely used in various macrophage-associated studies, while the similarities and differences in protein expression profiles between the two macrophage models are still largely unclear. In this study, the protein expression profiles of M1 and M2 phenotypes from both THP-1 and RAW264.7 macrophages were systematically investigated using mass spectrometry-based proteomics. By quantitatively analyzing more than 5,000 proteins among different types of macrophages (M0, M1 and M2) from both cell lines, we identified a list of proteins that were uniquely up-regulated in each macrophage type and further confirmed 43 proteins that were commonly up-regulated in M1 macrophages of both cell lines. These results revealed considerable divergences of each polarization type between THP-1 and RAW264.7 macrophages. Moreover, the mRNA and protein expression of CMPK2, RSAD2, DDX58, and DHX58 were strongly up-regulated in M1 macrophages for both macrophage models. These data can serve as important resources for further studies of macrophage-associated diseases in experimental pathology using human and mouse cell line models.

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“…The details of sample treatment were described previously ( 18 ). Treated and untreated cells were washed three times by ice-cold PBS buffer to remove the cell culture medium.…”

Section: Methodsmentioning

confidence: 99%

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

Zou

et al. 2021

Front. Immunol.

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“…Anyway, β-catenin transcriptionally inhibited fat mass and obesity-associated protein (FTO) via binding to its promoter region, and berberine could downregulate m 6 A methylation via decreasing β-catenin and increasing FTO [81]. Moreover, berberine inhibited colon cancer cell proliferation via modulating mitochondrial translation and ribosome biogenesis, as well as promoting calcium mobilization and fatsoluble vitamins metabolism [84]. In addition, CRC proliferation was related to elevating telomerase level and activity.…”

Section: Colorectal Cancermentioning

confidence: 99%

Anticancer Effects and Mechanisms of Berberine from Medicinal Herbs: An Update Review

Xiong

Huang

et al. 2022

Molecules

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Cancer has been a serious public health problem. Berberine is a famous natural compound from medicinal herbs and shows many bioactivities, such as antioxidant, anti-inflammatory, antidiabetic, anti-obesity, and antimicrobial activities. In addition, berberine shows anticancer effects on a variety of cancers, such as breast, lung, gastric, liver, colorectal, ovarian, cervical, and prostate cancers. The underlying mechanisms of action include inhibiting cancer cell proliferation, suppressing metastasis, inducing apoptosis, activating autophagy, regulating gut microbiota, and improving the effects of anticancer drugs. This paper summarizes effectiveness and mechanisms of berberine on different cancers and highlights the mechanisms of action. In addition, the nanotechnologies to improve bioavailability of berberine are included. Moreover, the side effects of berberine are also discussed. This paper is helpful for the prevention and treatment of cancers using berberine.

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“…MMP-16 is a target gene of miR-145. Berberine inhibits migration and invasion by promoting miR-145 expression and decreasing MMP-16 expression in the human ovarian cancer (SK-OV-3, 3AO) cells ( Li P. et al, 2021 ). Furthermore, berberine inhibits migration in breast cancer (ZR-75–30) cells by targeting ephrin-B2 ( Ma et al, 2017 ).…”

Section: Antitumor Effects and Corresponding Mechanismsmentioning

confidence: 99%

“…Proteomic data demonstrated differentially expressed proteins (DEPs) among 5,130 or 8,051 identified proteins on berberine-treated CRC DLD-1 (675 proteins), HCT-116 (865 proteins), Caco-2 (503 proteins), and LOVO (277 proteins) cells, respectively. Among these, a total of 54 DEPs (22 upregulated and 32 downregulated proteins) overlapped in DLD1 and HCT116 cells, as well as 83 DEPs (the most downregulated proteins) overlapped in Caco-2 and LOVO cells were mainly involved in specific pathways, including calcium mobilization (LAT2/NTAL/LAB), metabolism of fat-soluble vitamins (LDLR and VKORC1), mitochondrial protein synthesis (GTPase ERAL1, MRPL11, and MRPL 48), tricarboxylic acid cycle (TCA) (citrate synthase, CS), and respiratory electron transport pathway (NDUFS2 and COX7A2L) ( Tong et al, 2020 ; Li P. et al, 2021 ). Moreover, a total of 96 and 22 DEPs screened from 1,800 identified proteins showed altered protein expression and thiol reactivity on berberine-treated breast cancer (MCF-7) cells, respectively, which are attributable to gene regulation (HDAC1), protein folding (HSP27 and PPIA), signal transduction (KCIP-1 and NRG2), and metabolism (ENO1 and TPI1) ( Chou et al, 2012 ).…”

Section: Omics Studiesmentioning

confidence: 99%

Current Advances in Coptidis Rhizoma for Gastrointestinal and Other Cancers

Chen

Liang

et al. 2022

Front. Pharmacol.

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Cancer is a serious disease with an increasing number of reported cases and high mortality worldwide. Gastrointestinal cancer defines a group of cancers in the digestive system, e.g., liver cancer, colorectal cancer, and gastric cancer. Coptidis Rhizoma (C. Rhizoma; Huanglian, in Chinese) is a classical Chinese medicinal botanical drug for the treatment of gastrointestinal disorders and has been shown to have a wide variety of pharmacological activity, including antifungal, antivirus, anticancer, antidiabetic, hypoglycemic, and cardioprotective effects. Recent studies on C. Rhizoma present significant progress on its anticancer effects and the corresponding mechanisms as well as its clinical applications. Herein, keywords related to C. Rhizoma, cancer, gastrointestinal cancer, and omics were searched in PubMed and the Web of Science databases, and more than three hundred recent publications were reviewed and discussed. C. Rhizoma extract along with its main components, berberine, palmatine, coptisine, magnoflorine, jatrorrhizine, epiberberine, oxyepiberberine, oxyberberine, dihydroberberine, columbamine, limonin, and derivatives, are reviewed. We describe novel and classic anticancer mechanisms from various perspectives of pharmacology, pharmaceutical chemistry, and pharmaceutics. Researchers have transformed the chemical structures and drug delivery systems of these components to obtain better efficacy and bioavailability of C. Rhizoma. Furthermore, C. Rhizoma in combination with other drugs and their clinical application are also summarized. Taken together, C. Rhizoma has broad prospects as a potential adjuvant candidate against cancers, making it reasonable to conduct additional preclinical studies and clinical trials in gastrointestinal cancer in the future.

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Quantitative Proteomics Analysis of Berberine-Treated Colon Cancer Cells Reveals Potential Therapy Targets

Cited by 13 publications

References 72 publications

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

Anticancer Effects and Mechanisms of Berberine from Medicinal Herbs: An Update Review

Current Advances in Coptidis Rhizoma for Gastrointestinal and Other Cancers

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