Quantitative Proteomics Analysis of Global Protein Expression in Campylobacter jejuni Cultured in Sublethal Concentrations of Bile Acids and Varying Temperatures

Abstract

Campylobacter jejuni is the leading cause of diarrhoea among human beings worldwide.Epidemiological investigations have shown that it affects over 500 million people per year.C. jejuni is mainly transmitted to human through consumption of cross contaminated chicken.In most cases, the diarrhoea clears by itself within 3 to 5 days. But it causes a big discomfort in the affected individuals. In addition, it has a huge economic impact due to sick leaves.Because of this, efforts are being put into understanding how C. jejuni interacts with human beings and other hosts. An indepth understanding of how this pathogen interacts with its hosts will lead to development of appropriate diagnosis tools and prevention measures. Bile acids are a major component of the gut fluid in all the hosts of C. jejuni. However, the interaction of C. jejuni and different types of bile acids at human body temperature of 37 o C is poorly understood. Consequently, this study was designed to unearth the proteomic response in C. jejuni reference strain 81-176 to sublethal concentrations of cholic acid (CA), deoxycholic acid (DCA), lithocholic acid (LCA), taurocholic acid (TCA), chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA) and glycocholic acid (GCA). The specific objectives were: (i) to investigate the response in 81-176 to DCA 0.05% at 37 o C for 12h and 24h using both stable isotope labeling with amino acids in cell culture (SILAC) and label-free analysis with sequantial window acquisation of all theoretical mass spectra (SWATH); and determine a suitable quantitative method for the study. (ii) To use the method selected quantitative method to investigate global protein expression in 81-176 in response to sublethal concentrations of CA, LCA, TCA, CDCA, UDCA and GCA cultured at 37 o C for 12h under microaerophilic conditions. (iii) To identify and characterize a currently uncharacterized and widely induced protein (iv) To use label-free analysis with SWATH to investigate protein expression in 81-176 cultured in temperatures of 37 o C (human) and 42 o C (chicken) without bile acids.. Intially, the capability of C. jejuni to adhere and invade Caco-2 cells in the presence of various concentrations of bile acids was investigated using gentamicin protection assay (GPA). The results showed that DCA, CDCA and GCA promoted adherence and invasion in a dose depandant fashion. LCA and UDCA didn't neither promote nor suppress adherence and invasion. Subsequently, IC 50 of each bile acid was obtained. Half of this concentration of each bile acid corresponded to the concentrations that are present in the large intestines of human Microbiology and Genetics, GAUSS) and Prof. Dr. Holger Reichardt (Celluar and Molecular Immunology, UMG) for accepting to be members of my thesis committee and for their helpful suggestions. PD Dr. Andreas E. Zautner for having accepted me to be a Ph.D student in his group and for the support that he offered me throughout the study. Dr. Raimond Lugert, a senior scientist in the Campylobacter Research group for correcting ...