Quantitative proteomics analysis of young and elderly skin with DIA mass spectrometry reveals new skin aging-related proteins

Ma, Jing; Liu, Mengting; Wang, Yaochi; Xin, Cong; Zhang, Hui; Chen, Shirui; Zheng, Xiaodong; Zhang, Xuejun; Xiao, Feng‐Li; Yang, Sen

doi:10.18632/aging.103461

Cited by 26 publications

(18 citation statements)

References 119 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study on age-dependent variation in cytokines, chemokines, and biological analytes rinsed from the surface of healthy human skin showed significantly decreased levels of epidermal growth factor, fibroblast growth factor 2, IFN-α2, IL-1 receptor antagonist, HSA, keratin-6, and involucrin in aged skin, whereas cortisol levels were increased [ 40 ]. Moreover, several novel stratum corneum proteins related to chronic inflammation were upregulated in aged skin, including ORM1, a plasma-binding protein involved in the transport of inflammatory mediators [ 41 ].…”

Section: Keratinocyte Senescencementioning

confidence: 99%

Cellular Senescence and Inflammaging in the Skin Microenvironment

Lee

Choi

Roh

et al. 2021

IJMS

109

View full text Add to dashboard Cite

Cellular senescence and aging result in a reduced ability to manage persistent types of inflammation. Thus, the chronic low-level inflammation associated with aging phenotype is called “inflammaging”. Inflammaging is not only related with age-associated chronic systemic diseases such as cardiovascular disease and diabetes, but also skin aging. As the largest organ of the body, skin is continuously exposed to external stressors such as UV radiation, air particulate matter, and human microbiome. In this review article, we present mechanisms for accumulation of senescence cells in different compartments of the skin based on cell types, and their association with skin resident immune cells to describe changes in cutaneous immunity during the aging process.

show abstract

Section: Keratinocyte Senescencementioning

confidence: 99%

Cellular Senescence and Inflammaging in the Skin Microenvironment

Lee

Choi

Roh

et al. 2021

IJMS

109

View full text Add to dashboard Cite

show abstract

“…VAT1 is a membrane protein involved in vesicular transport and is overexpressed in both cancer , and hyperplasia . Notably, because VAT1 is downregulated in aged skin, it may represent an HMEC-specific senescence biomarker. Finally, PLS1/3 are actin-bundling proteins that have not, to our knowledge, been previously linked to either aging or senescence, although we speculate that PLS1/3 could regulate the cytoskeletal reorganization observed in senescence. , Future work is needed to understand how these novel biomarkers regulate HMEC senescence.…”

Section: Resultsmentioning

confidence: 99%

Identification of a Proteomic Signature of Senescence in Primary Human Mammary Epithelial Cells

et al. 2021

View full text Add to dashboard Cite

Senescence is a permanent cell cycle arrest that occurs in response to cellular stress and promotes age-related disease. Because senescence differs greatly depending on cell type and senescence inducer, continued progress in the characterization of senescent cells is needed. Here, we analyzed primary human mammary epithelial cells (HMECs), a model system for aging and cancer, using mass spectrometry-based proteomics. By integrating data from replicative senescence, immortalization by telomerase reactivation, and quiescence, we identified a robust proteomic signature of HMEC senescence consisting of 34 upregulated and 10 downregulated proteins. This approach identified known senescence biomarkers including β-galactosidase (GLB1) as well as novel senescence biomarkers including catechol O-methyltransferase (COMT), synaptic vesicle membrane protein VAT-1 homolog (VAT1), and plastin-1/3 (PLS1/PLS3). Gene ontology enrichment analysis demonstrated that senescent HMECs upregulated lysosomal proteins and downregulated RNA metabolic processes. In addition, a classification model based on our proteomic signature successfully discriminated proliferating and senescent HMECs at the transcriptional level. Finally, we found that the HMEC senescence signature was positively and negatively correlated with proteomic alterations in HMEC aging and breast cancer, respectively. Taken together, our results demonstrate the power of proteomics to identify cell type-specific signatures of senescence and advance the understanding of senescence in HMECs.

show abstract

“…TGFβ2 is a ligand of the transforming growth factor-beta superfamily (Baxter, 2000) and disruptions of the TGFβ/SMAD pathway has also been linked to disease and age-associated disorders (Tominaga & Suzuki, 2019). HPX and SERPINC1, proteins linked to the circulation and recently associated to Alzheimer’s disease (Ashraf et al, 2020) and aging of the skin (Ma et al, 2020), but whose roles in the dentin remain to be understood.…”

Section: Discussionmentioning

confidence: 99%

Proteomic pipeline to identify age-related compositional and structural changes of the human dentin extracellular matrix

Reis

Lee

Bedran-Russo

et al. 2020

Preprint

View full text Add to dashboard Cite

Aging is the conjunction of progressive physiological changes that occur in living organisms over time. A fundamental component of organs and tissues that undergoes functional and structural modifications with age is the extracellular matrix (ECM). The ECM is a dynamic assembly of proteins that regulates cellular functions and participates in tissue organization and remodeling. The ECM of teeth is no different but lacks turnover capability. Dentin, the bulk of the tooth, endures modifications throughout a lifespan. As of now, the composition of the dentin ECM and how it changes with age is unknown. To address, we used proteomics to profile the dentin ECM of young and older adult human teeth. We report the identification of 341 unique ECM proteins and define the matrisome of young and old dentin as composed of 125 and 112 proteins, respectively. Our study identified over 100 ECM proteins that have not previously been associated with the dentin. Importantly, it also reveals changes to both protein solubility and matrisome composition as a function of age. Our study is a first step towards the discovery of biomarkers of the aging tooth and the development of strategies to maintain or regenerate a healthy dentition in our aging population.

show abstract

Quantitative proteomics analysis of young and elderly skin with DIA mass spectrometry reveals new skin aging-related proteins

Cited by 26 publications

References 119 publications

Cellular Senescence and Inflammaging in the Skin Microenvironment

Cellular Senescence and Inflammaging in the Skin Microenvironment

Identification of a Proteomic Signature of Senescence in Primary Human Mammary Epithelial Cells

Proteomic pipeline to identify age-related compositional and structural changes of the human dentin extracellular matrix

Contact Info

Product

Resources

About