Quantitative Proteomics and Weighted Correlation Network Analysis of Tear Samples in Type 2 Diabetes Patients Complicated with Dry Eye

Zou, Xudong; Zhang, Pei; Xu, Yi; Lu, Lina; Zou, Haidong

doi:10.1002/prca.201900083

Cited by 10 publications

(6 citation statements)

References 23 publications

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“…Basal tear flow (Schirmer test II) and TBUT were found to be unaffected. These data are consistent with the observation that diabetic neuropathy most often involves the ophthalmic nerve, thus triggering a corneal neuropathy with loss of sensitivity [39,40], which in turn results in decreased tear secretion and altered composition of tear proteins [41,42].…”

Section: Discussionsupporting

confidence: 92%

Effects of the Topical Use of the Natural Antioxidant Alpha-Lipoic Acid on the Ocular Surface of Diabetic Patients with Dry Eye Symptoms

Roszkowska¹,

Spinella²,

Oliverio³

et al. 2022

Front. Biosci. (Landmark Ed)

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Purpose:The purpose of this study is to investigate the effects of the treatment with eye-drops based on a combination of antioxidant and mucomimetic molecules, namely 0.1% alpha-lipoic acid (ALA) and 0.3% hydroxy-propyl-methylcellulose (HPMC) on the ocular surface of diabetic patients with dry eye symptoms. Methods: Seventy patients, 42 M and 28 F, aged from 50 to79 years (mean 62.1 ± 10.5), affected by type II diabetes mellitus, were enrolled and divided in two groups treated for 2 months as follows: Group 1 (35 patients), received topical ALA/HPMC three times a day, Group 2 (35 patients) received topical HPMC (0.3%) alone, three times a day. The main outcome measures were: Ocular Surface Disease Index (OSDI), tear film break-up time (TBUT), corneal fluorescein staining, Schirmer I test, corneal sensitivity. An examination of tear film morphology with confocal microscopy was carried out in a subset of patients of each group at baseline and after two months. Statistical analysis was performed with t-test for the parametric data and Mann-Whitney U-test or chi-squared test for the nonparametric data. Results: Both treatments resulted in significant improvements of BUT, OSDI and tear film morphology, although the improvements observed in group 1 showed a higher trend than what observed for group 2. Moreover, only in group 1 a significant improvement was visible for corneal staining, and no significant improvements were observed in any group for Schirmer I and sensitivity. Conclusions: These results confirmed the efficacy of HPMC in the treatment of diabetic dry eye and indicated that the addition of a strong self-regenerating antioxidant like ALA may give a distinctive advantage for the healing of corneal defects (as evidenced by corneal staining), beside improving HPMC efficacy on three other parameters (BUT, OSDI score, tear morphology). Therefore, the addition of a strong antioxidant like ALA can be helpful in preventing or treating ocular surface defects in diabetic patients, in which the oxidative damage is predominant.

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Section: Discussionsupporting

confidence: 92%

Effects of the Topical Use of the Natural Antioxidant Alpha-Lipoic Acid on the Ocular Surface of Diabetic Patients with Dry Eye Symptoms

Roszkowska¹,

Spinella²,

Oliverio³

et al. 2022

Front. Biosci. (Landmark Ed)

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show abstract

“…Not discovering abnormally expressed gene signatures, WGCNA can assign highly correlated genes into the same coexpression module and identify clinical phenotype-related modules, which is more effective to identify diagnostic and therapeutic markers [ 9 ]. Recent studies have identified shared susceptibility modules and genes of diabetes and other diseases such as Alzheimer's disease [ 10 ], cardiovascular disease [ 11 ], and dry eye [ 12 ] utilizing WGCNA approach. Least Absolute Shrinkage and Selection Operator (LASSO) represents a popular technology, which is extended and broadly utilized to construct diagnostic and prognostic Cox proportional hazard regression models based on transcriptome profiles [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

[Retracted] Establishment and Verification of a Gene Signature for Diagnosing Type 2 Diabetics by WGCNA, LASSO Analysis, and In Vitro Experiments

Shao

Zhang

Liu

et al. 2022

BioMed Research International

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Objective. The incidence and prevalence of type 2 diabetes are increasing with age. Nevertheless, there is lack of sensitive diagnostic tools and effective therapeutic regimens. We aimed to establish and verify a practical and valid diagnostic tool for this disease. Methods. WGCNA was presented on the expression profiling of type 2 diabetic and normal islets in combined GSE25724 and GSE38642 datasets. By LASSO Cox regression analyses, a gene signature was constructed based on the genes in diabetes-related modules. ROC curves were plotted for assessing the diagnostic efficacy. Correlations between the genes and immune cell infiltration and pathways were analyzed. BST2 and BTBD1 expression was verified in glucotoxicity-induced and normal islet β cells. The influence of BST2 on β cell dysfunction was investigated under si-BST2 transfection. Results. Totally, 14 coexpression modules were constructed, and red and cyan modules displayed the correlations to diabetes. The LASSO gene signature (BST2, BTBD1, IFIT1, IFIT3, and RTP4) was developed. The AUCs in the combined datasets and GSE20966 dataset were separately 0.914 and 0.910, confirming the excellent performance in diagnosing type 2 diabetes. Each gene in the model was distinctly correlated to immune cell infiltration and key signaling pathways (TGF-β and P53, etc.). The abnormal expression of BST2 and BTBD1 was confirmed in glucotoxicity-induced β cells. BST2 knockdown ameliorated β cell dysfunction and altered the activation of TGF-β and P53 pathways. Conclusion. Our findings propose a gene signature with high efficacy to diagnose type 2 diabetes, which could assist and improve early diagnosis and therapy.

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“…Downregulation of these two genes supports the conclusion that 9,10-PQ treatment increased glycolysis capacity, as described above, and suggests that 9,10-PQ influenced osmotic pressure in fish liver. DNAJC3A and HYOU1, as members of heat shock proteins (HSP), are potential biomarkers of diabetes (92) and endoplasmic reticulum stress (61). Moreover, HYOU1 and NLRC3 play roles in proliferation and inflammation in cancer cells, and NLRC3 has been shown to be significantly downregulated in cancer tissues (38,90).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome changes ofTakifugu obscurusliver after acute exposure to the oxygenated-PAH 9,10-phenanthrenequione

Jiang

Yang

Fang

2020

Physiological Genomics

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Contamination with polycyclic aromatic hydrocarbons (PAHs) causes noticeable ecological problems in aquatic ecosystems. 9,10-phenanthrenequione (9,10-PQ) is an oxidized PAH and is highly toxic to aquatic animals. However, the effects of 9,10-PQ on the molecular metabolism of fish remain largely unknown. In this study, Takifugu obscurus juveniles were acutely exposed to 44.30 µg/L 9,10-PQ for three days. The transcriptome profile changes in their livers were compared between the 9,10-PQ treatment group and the control using T. rubripes as the reference genome. The results identified 22,414 genes in our transcriptome. Among them, 767 genes were differentially expressed (DEGs) after exposure to 9,10-PQ, which enriched 16 KEGG pathways. Among them, the glycolysis, phagosome, and FOXO signaling pathways were significantly activated in 9,10-PQ treatment compared with the control. These data indicate that 9,10-PQ increased the glycolysis capacity to produce more energy for resistance and harmed immune function. Moreover, several genes related to tumorigenesis were significantly up-regulated in response to 9,10-PQ, displaying the carcinogenic toxicity of 9,10-PQ to T. obscurus. Genes in steroid biosynthesis pathways were down-regulated in the 9,10-PQ treatment group, suggesting interference with the endocrine system. Overall, these findings provide information to help evaluate the environmental risks that oxygenated-PAHs present to T. obscurus.

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Quantitative Proteomics and Weighted Correlation Network Analysis of Tear Samples in Type 2 Diabetes Patients Complicated with Dry Eye

Cited by 10 publications

References 23 publications

Effects of the Topical Use of the Natural Antioxidant Alpha-Lipoic Acid on the Ocular Surface of Diabetic Patients with Dry Eye Symptoms

Effects of the Topical Use of the Natural Antioxidant Alpha-Lipoic Acid on the Ocular Surface of Diabetic Patients with Dry Eye Symptoms

[Retracted] Establishment and Verification of a Gene Signature for Diagnosing Type 2 Diabetics by WGCNA, LASSO Analysis, and In Vitro Experiments

Transcriptome changes ofTakifugu obscurusliver after acute exposure to the oxygenated-PAH 9,10-phenanthrenequione

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