Quantitative proteomics reveals protein kinases and phosphatases in the individual phases of contextual fear conditioning in the C57BL/6J mouse

Šmidák, Roman; Mayer, Rupert L.; Bileck, Andrea; Gerner, Christopher; Mechtcheriakova, Diana; Stork, Oliver; Lubec, Gert; Li, Lin

doi:10.1016/j.bbr.2015.12.033

Cited by 9 publications

(6 citation statements)

References 48 publications

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“…The sound should precede the shock, with an interval of 58 s between the two stimuli. The Panlab fear condition device was used to record the freezing time of mice in both contextual and cued tests, and all data were analyzed by PACKWIN v2.0.05 ( Šmidák et al, 2016 ; Tishkina et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Identification of Potential Key circRNAs in Aged Mice With Postoperative Delirium

Wei

Liang

Yuan

et al. 2022

Front. Mol. Neurosci.

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Postoperative delirium (POD) is a common postoperative complication in elderly patients and seriously affects postoperative recovery. The exact mechanism of POD is still unclear. Therefore, it is necessary to explore the mechanism of POD in transcriptional regulation. At present, circRNAs have been proven to play an important role in a variety of mental health and cognitive disorders, such as Alzheimer’s disease, depression and schizophrenia. To reveal the effect of circRNA on POD, we used microarray to analyze the differential expression profiles of circRNAs in the hippocampus of 12-month-old mice between the tibial fracture and control groups. A total of 1,4236 circRNAs were identified. Compared with the control group, there were 500 circRNAs with increased expression and 187 with decreased expression. The accuracy of the microarray data was further verified by qRT–PCR. Finally, GO enrichment and KEGG pathway analyses indicated that changes in axon orientation, ubiquitin-mediated proteolysis, glutamate synapses, the estrogen signaling pathway, the RAS signaling pathway and other systems may be important potential pathological mechanisms in the progression of POD. In particular, we found that the HOMER1 gene and its transcript mmu_circRNA_26701 are specifically expressed in the glutamate synapse, which may provide new clues and intervention targets for the progression of this refractory disease.

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Section: Methodsmentioning

confidence: 99%

Identification of Potential Key circRNAs in Aged Mice With Postoperative Delirium

Wei

Liang

Yuan

et al. 2022

Front. Mol. Neurosci.

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“…Generally, involvement of protein kinases and phosphatases in various learning paradigms has already been shown, but the exact function of TULA‐2 in conditioning remains unknown. One may hypothesize that the dephosphorylation of Syk plays an important role in this process, although it should also be noted that Syk itself is not listed in this study as a kinase changing its level in the course of conditioning (Smidak et al, ).…”

Section: Role Of Tula Proteins In Health and Diseasementioning

confidence: 96%

“…An example of such findings provides a proteomics study of the role of protein kinases and phosphatases in the development of contextual fear conditioning. It has been shown that the level of TULA‐2 increases specifically during the consolidation phase of conditioning and that this increase is one of the highest (~5‐fold) among the proteins studied (Smidak et al, ). Generally, involvement of protein kinases and phosphatases in various learning paradigms has already been shown, but the exact function of TULA‐2 in conditioning remains unknown.…”

Section: Role Of Tula Proteins In Health and Diseasementioning

confidence: 99%

TULA‐family proteins: Jacks of many trades and then some

Tsygankov

2018

Journal Cellular Physiology

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UBASH3/STS/TULA is a novel two-member family, which exerts several key regulatory effects in multiple cell types. UBASH3B/STS-1/TULA-2 is a highly active protein tyrosine phosphatase; its major target appears to be a specific regulatory site of protein tyrosine kinases of the Syk family, dephosphorylation of which inhibits Syk and Zap-70 kinases and suppresses receptor signaling mediated by these kinases. UBASH3A/STS-2/TULA exhibits substantial homology to UBASH3B/STS-1/TULA-2, but possesses only a small fraction of phosphatase activity of UBASH3B/STS-1/TULA-2, and thus, its regulatory effect may be based also on the phosphatase-independent mechanisms. Critical physiologic effects of these proteins have been demonstrated in T lymphocytes, platelets, stem cells, and other important cell types. These proteins have also been shown to play a key role in such pathologic conditions as autoimmunity, cancer, and thrombosis. The review focuses on the recent studies of this important family of cellular regulators.

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“…Thus, TULA-1 has been implicated in the regulation of human kidney development; in this process, TULA-1 may interact with AIF-related proteins [83]. Another example of an unexpected putative lymphoid-independent function of TULA-family proteins is provided by a strong and specific increase in the level of TULA-2 in mouse brain during the consolidation phase of conditioning [84].…”

Section: Ptp-independent Regulatory Effects Of Tula-family Proteinsmentioning

confidence: 99%

TULA Proteins in Men, Mice, Hens, and Lice: Welcome to the Family

Tsygankov

2023

IJMS

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The two members of the UBASH3/STS/TULA protein family have been shown to critically regulate key biological functions, including immunity and hemostasis, in mammalian biological systems. Negative regulation of signaling through immune receptor tyrosine-based activation motif (ITAM)- and hemITAM-bearing receptors mediated by Syk-family protein tyrosine kinases appears to be a major molecular mechanism of the down-regulatory effect of TULA-family proteins, which possess protein tyrosine phosphatase (PTP) activity. However, these proteins are likely to carry out some PTP-independent functions as well. Whereas the effects of TULA-family proteins overlap, their characteristics and their individual contributions to cellular regulation also demonstrate clearly distinct features. Protein structure, enzymatic activity, molecular mechanisms of regulation, and biological functions of TULA-family proteins are discussed in this review. In particular, the usefulness of the comparative analysis of TULA proteins in various metazoan taxa, for identifying potential roles of TULA-family proteins outside of their functions already established in mammalian systems, is examined.

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Quantitative proteomics reveals protein kinases and phosphatases in the individual phases of contextual fear conditioning in the C57BL/6J mouse

Cited by 9 publications

References 48 publications

Identification of Potential Key circRNAs in Aged Mice With Postoperative Delirium

Identification of Potential Key circRNAs in Aged Mice With Postoperative Delirium

TULA‐family proteins: Jacks of many trades and then some

TULA Proteins in Men, Mice, Hens, and Lice: Welcome to the Family

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