Quantitative Proteomics Using iTRAQ Labeling and Mass Spectrometry

Fuller, Heidi R.; Morris, George E.

doi:10.5772/31469

Integrative Proteomics 2012

DOI: 10.5772/31469

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Quantitative Proteomics Using iTRAQ Labeling and Mass Spectrometry

Heidi R. Fuller¹,

George E. Morris²

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2019

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Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs

Jimenez

McKenzie

et al. 2019

J. Proteome Res.

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Extracellular vesicles (EVs) are important mediators of cell-cell communication due to their cargo content of proteins, lipids and RNAs. We previously reported that small EVs (SEVs) called exosomes promote directed and random cell motility, invasion, and serum-independent growth. In contrast, larger EVs (LEVs) were not active in those assays, but may have unique functional properties. In order to identify protein cargos that may contribute to different functions of SEVs and LEVs, we used isobaric tag for relative and absolute quantitation (iTRAQ)-liquid chromatography (LC) tandem mass spectrometry (MS) on EVs isolated from a colon cancer cell line. Mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD010840. Bioinformatic analyses revealed that SEVs are enriched in proteins associated with cell-cell junctions, cell-matrix adhesion, exosome biogenesis machinery and various signaling pathways. In contrast, LEVs are enriched in proteins associated with ribosome and RNA biogenesis and processing, and metabolism. Western blot analysis of EVs purified from 2 different cancer cell types confirmed the enrichment of cell-matrix and cell-cell adhesion proteins in SEVs. Consistent with those data, we found that cells exhibit enhanced adhesion to surfaces coated with *

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Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs

Jimenez

McKenzie

et al. 2019

J. Proteome Res.

View full text Add to dashboard Cite

show abstract

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Quantitative Proteomics Using iTRAQ Labeling and Mass Spectrometry

Cited by 1 publication

References 38 publications

Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs

Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs

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