Quantitative rather than qualitative differences in gene expression predominate in intestinal cell maturation along distinct cell lineages

Velcich, Anna; Corner, Georgia; Paul, D.; Zhuang, Min; Mariadason, John M.; Laboisse, Christian L.; Augenlicht, Leonard H.

doi:10.1016/j.yexcr.2004.10.014

Cited by 18 publications

(23 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4E). Interestingly, there was more modest induction of SBP1 in HT29 cl.19A cells, another clonal derivative of HT29 cells that differentiate into deep-crypt chloride-secreting cells over 21 days in culture [23,33], consistent with the sequential maturation of intestinal cells as they migrate along the crypt axis in the colon. These data suggest that SBP1 expression is upregulated during intestinal cell differentiation, although the extent of upregulation may vary in different intestinal cell types.…”

Section: Sbp1 Is Upregulated During In Vivo Intestinalmentioning

confidence: 66%

See 1 more Smart Citation

Expression of selenium‐binding protein 1 characterizes intestinal cell maturation and predicts survival for patients with colorectal cancer

Li¹,

Yang

et al. 2008

Molecular Nutrition Food Res

115

View full text Add to dashboard Cite

To identify candidate genes involved in the development of colorectal cancer, we used cDNA microarrays to analyze gene expression differences between human colorectal tumors and paired adjacent normal mucosa. We identified approximately 3.5-fold significant downregulation of selenium-binding protein 1 (SBP1) in colorectal tumors compared to normal mucosa (p = 0.003). Importantly, stage III colorectal cancer patients with low tumor-SBP1 expression had significantly shorter disease-free and overall survival as compared with those patients with high tumor-SBP1 expression (p = 0.04 and 0.03, respectively). We further characterized the role of SBP1 in colorectal cancer in vivo and in vitro. In normal tissue, SBP1 was maximally expressed in terminally differentiated epithelial cells on the luminal surface of crypts in the large intestine. Consistent with this in vivo localization, SBP1 was upregulated during in vitro colonic cell differentiation along the absorptive (Caco-2) and secretory (HT29 Clones 16E and 19A) cell lineages. Downregulation (approximately 50%) of SBP1 expression by small interfering RNA in colonic cancer cells was associated with reduced expression of another epithelial differentiation marker, carcinoembryonic antigen (CEA), although PCNA and p21(WAF1/cip1 )expression were not altered. These data demonstrate that higher expression of SBP1 is associated with differentiation of the normal colonic epithelia and may be a positive prognostic factor for survival in stage III colorectal carcinoma.

show abstract

Section: Sbp1 Is Upregulated During In Vivo Intestinalmentioning

confidence: 66%

“…For spontaneous differentiation studies, Caco-2, HT29 cl.16E, and HT29 cl.19A cells were cultured to confluence (day 0), and for 2, 5, 7, 14, or 21 days postconfluence, with medium changed every other day as previously described [22,23]. Cells were harvested in PBS at different time points.…”

Section: Cell Linesmentioning

confidence: 99%

Expression of selenium‐binding protein 1 characterizes intestinal cell maturation and predicts survival for patients with colorectal cancer

Li¹,

Yang

et al. 2008

Molecular Nutrition Food Res

115

View full text Add to dashboard Cite

show abstract

“…Since we have previously shown that Wnt signaling, a putative inducer of Jagged1, decreased Jagged1 inhibits colon cancer cell differentiation S Guilmeau et al during spontaneous growth arrest and differentiation of these HT29Cl16E cells (Velcich et al, 2005), we investigated the role of Jagged1. Figure 3b shows that Jagged1 levels decreased along with downregulation of NICD1 and NICD2, and Hes1, which paralleled our previously reported decreases in Wnt signaling and tumorigenicity of the HT29Cl16E cells (Velcich et al, 2005), consistent with a role for Jagged1 in active Notch signaling in the transformed cells. To investigate this, endogenous Jagged1 mRNA expression was reduced by 45%, 72 h after transfection of siRNA targeting Jagged1 (Figure 5d).…”

Section: Resultsmentioning

confidence: 99%

Heterogeneity of Jagged1 expression in human and mouse intestinal tumors: implications for targeting Notch signaling

et al. 2009

View full text Add to dashboard Cite

“…cDNA microarrays consisting of 8,063 clones were used to identify 257 differentially expressed genes in the C116E cells and 330 genes in the C119A cells measured at six time points over a 20-day period. Velcich et al [75] observed changes in numerous cell cycle regulation genes as well as decreased expression of c-myc correlating with the transition from proliferating to differentiated cells. The authors noted that while only 115 genes were common to the differentially expressed sets of the two cell lines, closer examination revealed that the overall expression profi les in the two clones were strikingly similar.…”

Section: Characterization Of Intestinal Epithelial Cell Culturesmentioning

confidence: 99%

“…Velcich et al [75] used microarrays to demonstrate that although two subclones of HT29 colon tumor cells (C116E and C119A) differentiate into different phenotypes, they exhibit similar gene expression programming during differentiation. C116E cells spontaneously differentiated into mucus-producing goblet cells, while C119A cells attain a deep-crypt secretory phenotype.…”

Section: Characterization Of Intestinal Epithelial Cell Culturesmentioning

confidence: 99%

Intestinal Epithelial Cells In Vitro

Chopra

Dombkowski

Stemmer

et al. 2010

Stem Cells and Development

View full text Add to dashboard Cite

Recent advances in the biology of stem cells has resulted in signifi cant interest in the development of normal epithelial cell lines from the intestinal mucosa, both to exploit the therapeutic potential of stem cells in tissue regeneration and to develop treatment models of degenerative disorders of the digestive tract. However, the diffi culty of propagating cell lines of normal intestinal epithelium has impeded research into the molecular mechanisms underlying differentiation of stem/progenitor cells into the various intestinal lineages. Several short-term organ/organoid and epithelial cell culture models have been described. There is a dearth of long-term epithelial and/or stem cell cultures of intestine. With an expanding role of stem cells in the treatment of degenerative disorders, there is a critical need for additional efforts to develop in vitro models of stem/progenitor epithelial cells of intestine. The objective of this review is to recapitulate the current status of technologies and knowledge for in vitro propagation of intestinal epithelial cells, markers of the intestinal stem cells, and gene and protein expression profi les of the intestinal cellular differentiation.

show abstract

Quantitative rather than qualitative differences in gene expression predominate in intestinal cell maturation along distinct cell lineages

Cited by 18 publications

References 53 publications

Expression of selenium‐binding protein 1 characterizes intestinal cell maturation and predicts survival for patients with colorectal cancer

Expression of selenium‐binding protein 1 characterizes intestinal cell maturation and predicts survival for patients with colorectal cancer

Heterogeneity of Jagged1 expression in human and mouse intestinal tumors: implications for targeting Notch signaling

Intestinal Epithelial Cells In Vitro

Contact Info

Product

Resources

About