Quantitative Real-Time Analysis of Living Materials by Stimulated Raman Scattering Microscopy

Qian, Chenxi; Liu, Hanwei; Chittur, Priya K.; Chadha, Rahuljeet S.; Yao, Yuxing; Kornfield, Julia A.; Tirrell, David A.; Wei, Lu

doi:10.1021/acs.analchem.3c03736

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…Beyond their versatility in detection, ELMs provide enhanced accuracy in quantitative analysis by responding to specific conditions . Advancements in in vivo functional materials have led to the development of implantable or wearable devices capable of continuously monitoring physiological states and administering medication as needed. ,, As nanotechnology and bioengineering progress, the application of ELMs in biosensors is poised to expand further, offering new avenues for rapid, convenient, and highly accurate detection.…”

Section: Applications Of Elmsmentioning

confidence: 99%

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Lu,

Huang,

Cui

et al. 2024

ACS Synth. Biol.

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Engineered Living Materials (ELMs) are materials composed of or incorporating living cells as essential functional units. These materials can be created using bottom-up approaches, where engineered cells spontaneously form welldefined aggregates. Alternatively, top-down methods employ advanced materials science techniques to integrate cells with various kinds of materials, creating hybrids where cells and materials are intricately combined. ELMs blend synthetic biology with materials science, allowing for dynamic responses to environmental stimuli such as stress, pH, humidity, temperature, and light. These materials exhibit unique "living" properties, including self-healing, self-replication, and environmental adaptability, making them highly suitable for a wide range of applications in medicine, environmental conservation, and manufacturing. Their inherent biocompatibility and ability to undergo genetic modifications allow for customized functionalities and prolonged sustainability. This review highlights the transformative impact of ELMs over recent decades, particularly in healthcare and environmental protection. We discuss current preparation methods, including the use of endogenous and exogenous scaffolds, living assembly, 3D bioprinting, and electrospinning. Emphasis is placed on ongoing research and technological advancements necessary to enhance the safety, functionality, and practical applicability of ELMs in real-world contexts.

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Section: Applications Of Elmsmentioning

confidence: 99%

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Lu,

Huang,

Cui

et al. 2024

ACS Synth. Biol.

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Mapping Endothelial-Macrophage Interactions in Diabetic Vasculature: Role of TREM2 in Vascular Inflammation and Ischemic Response

Malhi,

Luo,

Tang

et al. 2024

Preprint

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Vasculopathies occur 15 years earlier in individuals with diabetes mellitus (DM) as compared to those without, but the underlying mechanisms driving diabetic vasculopathy remain incompletely understood. Endothelial cells (ECs) and macrophages (MΦ) are critical players in vascular wall and their crosstalk is crucial in diabetic vasculopathy. In diabetes, EC activation enables monocyte recruitment, which transmigrate into the intima and differentiate into macrophages (MΦ). Beyond this established model of diapedesis, EC-MΦ interplay is highly intricate and heterogenous. To capture these highly context dependent EC-MΦ interactions, we leveraged single-cell (sc)RNA-seq in conjunction with spatial transcriptome (ST)-seq profiling to analyze human mesenteric arteries from non-diabetic (ND) and type 2 diabetic (T2D) donors. We provide in this study a transcriptomic map encompassing major arterial vascular cells, e.g., EC, mononuclear phagocyte (MP), and T cells, and their interactions associated with human T2D. Furthermore, we identified Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) as a top T2D-induced gene in MP, with concomitant increase of TREM2 ligands in ECs. TREM2 induction was confirmed in mouse models of T2D and monocyte/MΦ subjected to DM- mimicking stimuli. Perturbing TREM2 with either an antibody or silencing RNA in MPs led to decreased pro-inflammatory responses in MPs and ECs and increased EC migration in vitro. In a mouse model of diabetes, TREM2 expression and its interaction with ECs are increased in the ischemic, as compared to non-ischemic muscles. Importantly, neutralization of TREM2 using a neutralizing antibody enhanced ischemic recovery and flow reperfusion in the diabetic mice, suggesting a role of TREM2 in promoting diabetic PAD. Finally, we verified that both TREM2 expression and the TREM2-EC-interaction are increased in human patients with DM-PAD. Collectively, our study presents the first atlas of human diabetic vessels with a focus on EC-MP interactions. Exemplified by TREM2, our study provides valuable insights into EC-MΦ interactions, key processes contributing to diabetic vasculopathies and the potential of targeting these interactions for therapeutic development.

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Quantitative Real-Time Analysis of Living Materials by Stimulated Raman Scattering Microscopy

Cited by 2 publications

References 26 publications

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Mapping Endothelial-Macrophage Interactions in Diabetic Vasculature: Role of TREM2 in Vascular Inflammation and Ischemic Response

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