Quantitative real-time optical imaging of the tissue metabolic rate of oxygen consumption

Ghijsen, Michael; Lentsch, Griffin; Gioux, Sylvain; Brenner, Matthew; Durkin, Anthony J.; Choi, Bernard; Tromberg, Bruce J.

doi:10.1117/1.jbo.23.3.036013

Cited by 45 publications

(48 citation statements)

References 50 publications

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“…In this work, CMRO 2 was computed differently for the two cases of normoglycemia and hyperglycemia using the following formulas as described in detail in Ref. ,

{CMRO}_{2} \propto ({, \begin{array}{c} α \times CBF \times Hb : normal \\ 4 \times d ((), Hbr) / italicdt : hyper \end{array})

…”

Section: Methodsmentioning

confidence: 99%

Decreased cerebral blood flow and hemodynamic parameters during acute hyperglycemia in mice model observed by dual‐wavelength speckle imaging

Shemesh

Bokobza

Rozenberg

et al. 2019

Journal of Biophotonics

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In this study, we use dual-wavelength optical imaging-based laser speckle technique to assess cerebral blood flow and metabolic parameters in a mouse model of acute hyperglycemia (high blood glucose). The effect of acute glucose levels on physiological processes has been extensively described in multiple organ systems such as retina, kidney, and others. We postulated that hyperglycemia also alters brain function, which in turn can be monitored optically using dual-wavelength laser speckle imaging (DW-LSI) platform. DW-LSI is a wide-field, noncontact optical imaging modality that integrates the principles of laser flowmetry and oximetry to obtain macroscopic information such as hemoglobin concentration and blood flow. A total of eight mice (C57/BL6) were used, randomized into two groups of normoglycemia (control, n = 3) and hyperglycemia (n = 5). Hyperglycemia was induced by intraperitoneal injection of a commonly used anesthetic drug combining ketamine and xylazine (KX combo). We found that this KX combo increases blood glucose (BG) levels from 150 to 350 mg/dL, approximately, when measured 18 minutes post-administration. BG continues to increase throughout the test period, with BG reaching an average of 463 ± 20.34 mg/dL within 60 minutes. BG levels were measured every 10 minutes from tail blood using commercially available glucometer. Experimental results demonstrated reductions in cerebral blood flow (CBF) by 55%, tissue oxygen saturation (SO 2 ) by 15%, and cerebral metabolic rate of oxygen (CMRO 2 ) by 75% following acute hyperglycemia. The observed decrease in these parameters was consistent with results reported in the literature, measured by a variety of experimental techniques. Measurements with laser Doppler flowmetry (LDF) were also performed which confirmed a reduction in CBF following acute hyperglycemia. In summary, our findings indicate that acute hyperglycemia modified brain hemodynamic response and induced significant David Shemesh and Naor Bokovza contributed equally to this work.

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“…In this work, CMRO 2 was computed differently for the two cases of normoglycemia and hyperglycemia using the following formulas as described in detail in Ref. ,

{CMRO}_{2} \propto ({, \begin{array}{c} α \times CBF \times Hb : normal \\ 4 \times d ((), Hbr) / italicdt : hyper \end{array})

…”

Section: Methodsmentioning

confidence: 99%

Decreased cerebral blood flow and hemodynamic parameters during acute hyperglycemia in mice model observed by dual‐wavelength speckle imaging

Shemesh

Bokobza

Rozenberg

et al. 2019

Journal of Biophotonics

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“…76 , [80][81][82] The concept has been extended to real-time measurements using SSOP combined with speckle flow measurement into coherent-SSOP (c-SSOP), which has been validated onto arm-cuff occlusions in human subject and is now tested clinically. 83,84…”

Section: Sfdi and Specklementioning

confidence: 99%

Spatial frequency domain imaging in 2019: principles, applications, and perspectives

2019

Self Cite

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Spatial frequency domain imaging (SFDI) has witnessed very rapid growth over the last decade, owing to its unique capabilities for imaging optical properties and chromophores over a large field-of-view and in a rapid manner. We provide a comprehensive review of the principles of this imaging method as of 2019, review the modeling of light propagation in this domain, describe acquisition methods, provide an understanding of the various implementations and their practical limitations, and finally review applications that have been published in the literature. Importantly, we also introduce a group effort by several key actors in the field for the dissemination of SFDI, including publications, advice in hardware and implementations, and processing code, all freely available online.

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“…This quantity is equivalent to the concentration of deoxygenated hemoglobin that arrived in a venule following oxygen extraction by the brain. Therefore, within our measurement paradigm [6], Eq. (4) is re-written as:…”

Section: Absolute Cerebral Metabolic Rate Of Oxygen (Cmro 2 ) Calculamentioning

confidence: 99%

“…Diffuse optical imaging (DOI) techniques are an inroad to addressing this problem, as they are noncontact and use non-harmful visible and near-infrared light to probe tissue absorption and scattering for endogenous contrast. Recently, we demonstrated that the combination of Spatial Frequency Domain Imaging (SFDI) and Laser Speckle Imaging (LSI)) can quantify tissue metabolic changes with high spatial and temporal resolution [6]. We also recently applied high-speed LSI [7] and SFDI [8] to measure perfusion, oxygenation, and tissue scattering in the brain in a cardiac arrest (CA) model of global cerebral ischemia.…”

Section: Introductionmentioning

confidence: 99%

High-speed quantitative optical imaging of absolute metabolism in the rat cortex

Wilson

Crouzet

Torabzadeh

et al. 2019

Preprint

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Quantitative measures of blood flow and metabolism are essential for improved assessment of brain health and response to ischemic injury. In this report, we demonstrate a multimodal technique for measuring the cerebral metabolic rate of oxygen (CMRO 2 ) in the rodent brain on an absolute scale (M O 2 / min). We use laser speckle imaging (LSI) at 809 nm and spatial frequency domain imaging (SFDI) at 655 nm, 730 nm, and 850 nm to obtain spatiotemporal maps of cerebral blood flow (CBF), tissue absorption ( a ), and tissue scattering ( s '). Knowledge of these three values enables calculation of a characteristic blood flow speed, which in turn is input to a mathematical model with a "zero-flow" boundary condition to calculate absolute CMRO 2 . We apply this method to a rat model of cardiac arrest (CA) and cardiopulmonary resuscitation. With this model, the zero-flow condition occurs during entry into CA. The CMRO 2 values calculated with our method are in good agreement with those measured with magnetic resonance (MR) and positron emission tomography (PET) by other groups. Our technique provides a quantitative metric of cerebral metabolism that can potentially be used for comparison between animals and longitudinal monitoring of a single animal over multiple days, to assess differences in baseline metabolism and track recovery of metabolism in survival studies following ischemia and reperfusion.

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Quantitative real-time optical imaging of the tissue metabolic rate of oxygen consumption

Cited by 45 publications

References 50 publications

Decreased cerebral blood flow and hemodynamic parameters during acute hyperglycemia in mice model observed by dual‐wavelength speckle imaging

Decreased cerebral blood flow and hemodynamic parameters during acute hyperglycemia in mice model observed by dual‐wavelength speckle imaging

Spatial frequency domain imaging in 2019: principles, applications, and perspectives

High-speed quantitative optical imaging of absolute metabolism in the rat cortex

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