Quantitative Real-Time PCR Detection of TRPV1–4 Gene Expression in Human Leukocytes from Healthy and Hyposensitive Subjects

Spinsanti, Giacomo; Zannolli, Raffaella; Panti, Cristina; Ceccarelli, Ilaria; Marsili, Letizia; Bachiocco, Valeria; Frati, Francesco; Aloisi, Anna Maria

doi:10.1186/1744-8069-4-51

Cited by 69 publications

(52 citation statements)

References 38 publications

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“…TRPV1 is also expressed in the CNS, including the spinal cord, striatum, hippocampus, cerebellum, and amygdala (19)(20)(21)(22)(23). Finally, TRPV1 is found in nonneuronal cells, including leukocytes, smooth muscle cells, and endothelial cells (11,17,(24)(25)(26)(27). In this regard, TRPV1 activation has been reported to regulate the activation and proinflammatory properties of CD4 + T cells (28) NADA and TRPV1 have overlapping distributions, because NADA has been detected in the striatum, cerebellum, hippocampus, thalamus, brainstem, and dorsal root ganglia (7,19,20,23,29).…”

Section: T He Endogenous Lipid N-arachidonoyl Dopamine (Nada)mentioning

confidence: 99%

N-Arachidonoyl Dopamine Modulates Acute Systemic Inflammation via Nonhematopoietic TRPV1

Lawton

Tran

et al. 2017

The Journal of Immunology

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N-Arachidonoyl dopamine (NADA) is an endogenous lipid that potently activates the transient receptor potential vanilloid 1 (TRPV1), which mediates pain and thermosensation. NADA is also an agonist of cannabinoid receptors 1 and 2. We have reported that NADA reduces the activation of cultured human endothelial cells by LPS and TNF-α. Thus far, in vivo studies using NADA have focused on its neurologic and behavioral roles. In this article, we show that NADA potently decreases in vivo systemic inflammatory responses and levels of the coagulation intermediary plasminogen activator inhibitor 1 in three mouse models of inflammation: LPS, bacterial lipopeptide, and polymicrobial intra-abdominal sepsis. We also found that the administration of NADA increases survival in endotoxemic mice. Additionally, NADA reduces blood levels of the neuropeptide calcitonin gene-related peptide but increases the neuropeptide substance P in LPS-treated mice. We demonstrate that the anti-inflammatory effects of NADA are mediated by TRPV1 expressed by nonhematopoietic cells and provide data suggesting that neuronal TRPV1 may mediate NADA’s anti-inflammatory effects. These results indicate that NADA has novel TRPV1-dependent anti-inflammatory properties and suggest that the endovanilloid system might be targeted therapeutically in acute inflammation.

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Section: T He Endogenous Lipid N-arachidonoyl Dopamine (Nada)mentioning

confidence: 99%

N-Arachidonoyl Dopamine Modulates Acute Systemic Inflammation via Nonhematopoietic TRPV1

Lawton

Tran

et al. 2017

The Journal of Immunology

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“…Jacob y col. 23 (2013) recomiendan el uso de más de un gen de referencia, así como también de más de un algoritmo para la evaluación de la estabilidad de la expresión. En el presente trabajo, se comparan dos metodologías (NormFinder y Rest 2009) ampliamente usadas [24][25][26][27][28][29] para evaluar siete genes de referencia candidatos, con el fin de seleccionar aquellos de mayor estabilidad para estudios de calidad seminal.…”

Section: Discussionunclassified

Selección de genes de referencia en semen equino criopreservado para su uso en estudios de expresión genética con la técnica de PCR cuantitativa

Rico¹,

Castejón²,

Sánchez³

et al. 2014

Sanid. Mil.

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“…As such, it is clear that inflammatory cells have a central role in this entity. Since TRPV4 is expressed in several immune lineages (Damann et al 2009;Spinsanti et al 2008), researchers have also probed the role of TRPV4 in the cells of the innate immune system during ARDS. In a series of studies in isolated perfused mouse lungs, TRPV4 knockout resulted in decreased sensitivity to VILI (Hamanaka et al 2010).…”

Section: Acute Lung Injurymentioning

confidence: 99%

TRPV4: physiological role and therapeutic potential in respiratory diseases

Goldenberg

Ravindran

Kuebler

2014

Naunyn-Schmiedeberg's Arch Pharmacol

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Members of the family of transient receptor potential (TRP) channels have been implicated in the pathophysiology of a host of lung diseases. The role of these multimodal cation channels in lung homeostasis is thought to stem from their ability to respond to changes in mechanical stimuli (i.e., shear and stretch), as well as to various protein and lipid mediators. The vanilloid subfamily member, TRPV4, which is highly expressed in the majority of lung cell types, is well positioned for critical involvement in several pulmonary conditions, including edema formation, control of pulmonary vascular tone, and the lung response to local or systemic inflammatory insults. In recent years, several pharmacological inhibitors of TRPV4 have been developed, and the current generation of compounds possess high affinity and specificity for TRPV4. As such, we have now entered a time where the therapeutic potential of TRPV4 inhibitors can be systematically examined in a variety of lung diseases. Due to this fact, this review seeks to describe the current state of the art with respect to the role of TRPV4 in pulmonary homeostasis and disease, and to highlight the current and future roles of TRPV4 inhibitors in disease treatment. We will first focus on genera aspects of TRPV4 structure and function, and then will discuss known roles for TRPV4 in pulmonary diseases, including pulmonary edema formation, pulmonary hypertension, and acute lung injury. Finally, both promising aspects and potential pitfalls of the clinical use of TRPV4 inhibitors will be examined.

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Quantitative Real-Time PCR Detection of TRPV1–4 Gene Expression in Human Leukocytes from Healthy and Hyposensitive Subjects

Cited by 69 publications

References 38 publications

N-Arachidonoyl Dopamine Modulates Acute Systemic Inflammation via Nonhematopoietic TRPV1

N-Arachidonoyl Dopamine Modulates Acute Systemic Inflammation via Nonhematopoietic TRPV1

Selección de genes de referencia en semen equino criopreservado para su uso en estudios de expresión genética con la técnica de PCR cuantitativa

TRPV4: physiological role and therapeutic potential in respiratory diseases

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