Quantitative real-time PCR detection of adenovirus in clinical blood specimens: A comparison of plasma, whole blood and peripheral blood mononuclear cells

Perlman, Jason; Gibson, C.; Pounds, Stanley; Gu, Zheng; Bankowski, Matthew J.; Hayden, Randall T.

doi:10.1016/j.jcv.2007.09.003

Cited by 24 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This frequency is similar to that found in the DAISY study [10] (although in that study a visit was deemed positive if serum, saliva or stool samples were positive) and is twofold higher than that of the Finnish DIPP (Diabetes Prediction and Prevention) study (4% and 3% sera positivity in case and control participants) [7]. Our high enterovirus frequency may thus also support the notion of the non-inferiority or superiority of whole blood compared with other sources of nucleic acid, as previously observed in testing for other viruses [19,20].…”

Section: Discussionsupporting

confidence: 65%

Enterovirus RNA in longitudinal blood samples and risk of islet autoimmunity in children with a high genetic risk of type 1 diabetes: the MIDIA study

et al. 2014

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 65%

Enterovirus RNA in longitudinal blood samples and risk of islet autoimmunity in children with a high genetic risk of type 1 diabetes: the MIDIA study

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Adenovirus is not commonly found in peripheral blood, except during a fulminant infection (Flomenberg et al, 1997;Perlman et al, 2007). By contrast, a large majority of lymphocyte samples isolated from the adenoids or tonsils of normal children undergoing tonsillectomy or adenoidectomy contain adenovirus DNA (Garnett et al, 2002(Garnett et al, , 2009).…”

Section: Discussionmentioning

confidence: 99%

Adenovirus DNA in Guthrie cards from children who develop acute lymphoblastic leukaemia (ALL)

et al. 2010

View full text Add to dashboard Cite

BACKGROUND: In search of a proposed viral aetiology of childhood acute lymphoblastic leukaemia (ALL), the common species C adenoviruses were analysed in Guthrie cards. METHODS: Guthrie cards from 243 children who later developed ALL and from 486 matched controls were collected and analysed by nested polymerase chain reaction for the presence of adenovirus DNA. RESULTS: Adenovirus DNA was reliably detected from only two subjects, both of whom developed ALL. CONCLUSION: Adenovirus DNA is detected in Guthrie card samples at too low a frequency to reveal an association between adenovirus and the development of leukaemia.

show abstract

“…Blood samples from rhesus macaques were collected at 0, 5, 10, 15, 30, 60, 90 and 180 min post-injection with Ad5-LFA-3/IgG 1 using EDTA-Na as an anticoagulant [10]. Rat heart, spleen, liver, lung, kidney, gonad, brain, lymph node and thymus tissues were harvested at 10, 30 and 120 min post-injection with Ad5-LFA-3/IgG 1 .…”

Section: Preparation and Extraction Of Samplesmentioning

confidence: 99%

“…For a negative control, at least one no-template-control (NTC; reactions that contained all PCR components except for vector DNA) was included in each PCR run to confirm the absence of both template and product contamination in the PCR mixture [4,10,12]. As a positive control, a PCR reaction containing the Ad5-LFA-3/IgG 1 plasmid DNA was included in all runs.…”

Section: Preparation and Extraction Of Samplesmentioning

confidence: 99%

Development and Application of a Real-time PCR Method for Pharmacokinetic and Biodistribution Studies of Recombinant Adenovirus

Shui

Chen

et al. 2009

Mol Biotechnol

View full text Add to dashboard Cite

A replication-deficient recombinant adenovirus (Ad5-LFA-3/IgG(1)) that encodes secreted LFA-3/IgG(1) was constructed for gene therapy treatment of psoriasis. The purpose of this study was to develop a real-time PCR method for pharmacokinetic and biodistribution studies of Ad5-LFA-3/IgG(1) within the circulation and organs. This method showed good specificity, sensitivity and reproducibility over a wide dynamic range of concentrations. Quantitative measurement of recombinant adenoviral DNA suggested that the level of Ad5-LFA-3/IgG(1) DNA in circulating blood peaked within 10 min following intravenous injection in rhesus macaques. Following this peak, the adenoviral DNA level dropped significantly to a very low level. Real-time PCR revealed that Ad5-LFA-3/IgG(1) DNA was enriched in the spleen, lung and liver after injection of the adenovirus into rats through the tail vein. The adenoviral DNA was barely detected in other tissues. These data provide important information for clinical trials of Ad5-LFA-3/IgG(1) and confirm the utility of the real-time PCR assay for monitoring gene therapy trials.

show abstract

Quantitative real-time PCR detection of adenovirus in clinical blood specimens: A comparison of plasma, whole blood and peripheral blood mononuclear cells

Cited by 24 publications

References 23 publications

Enterovirus RNA in longitudinal blood samples and risk of islet autoimmunity in children with a high genetic risk of type 1 diabetes: the MIDIA study

Enterovirus RNA in longitudinal blood samples and risk of islet autoimmunity in children with a high genetic risk of type 1 diabetes: the MIDIA study

Adenovirus DNA in Guthrie cards from children who develop acute lymphoblastic leukaemia (ALL)

Development and Application of a Real-time PCR Method for Pharmacokinetic and Biodistribution Studies of Recombinant Adenovirus

Contact Info

Product

Resources

About