Quantitative redox proteomics revealed molecular mechanisms of salt tolerance in the roots of sugar beet monomeric addition line M14

Abstract

Background Salt stress is often associated with excessive production of reactive oxygen species (ROS). Oxidative stress caused by the accumulation of ROS is a major factor that negatively affects crop growth and yield. Root is the primary organ that senses and transmits the salt stress signal to the whole plant. How oxidative stress affect redox sensitive proteins in the roots is not known. Results In this study, the redox proteome of sugar beet M14 roots under salt stress was investigated. Using iTRAQ reporters, we determined that salt stress caused significant changes in the abundance of many proteins (2305 at 20 min salt stress and 2663 at 10 min salt stress). Using iodoTMT reporters, a total of 95 redox proteins were determined to be responsive to salt stress after normalizing again total protein level changes. Notably, most of the differential redox proteins were involved in metabolism, ROS homeostasis, and stress and defense, while a small number play a role in transport, biosynthesis, signal transduction, transcription and photosynthesis. Transcription levels of 14 genes encoding the identified redox proteins were analyzed using qRT-PCR. All the genes were induced by salt stress at the transcriptional level. Conclusions Based on the redox proteomics results, we construct a map of the regulatory network of M14 root redox proteins in response to salt stress. This study further refines the molecular mechanism of salt resistance at the level of protein redox regulation.

Comparative proteomic analysis on chloroplast proteins provides new insights into the effects of low temperature in sugar beet

“…In sugar beet, a total of 570 proteins were identified in vernalized and nonvernalized plants, of which the majority were assigned to phenylpropanoid biosynthesis, hormone metabolism and protein processing pathway (Liang et al 2018 ). Several hundreds of proteins involved in photosynthesis and ROS homeostasis were also found in response to drought, salt stress and alkali stress in sugar beet (Yang et al 2013 ; Wang et al 2017 ; Geng et al 2021 ; Liu et al 2022 ). However, the response of the chloroplast proteome to low temperature has not been studied in sugar beet.…”