Poyang Lake is the largest freshwater lake in China and forms an essential component of the hydrological, nutrient, and carbon cycles, providing various ecosystem services to the local environment. Since changes in Poyang Lake’s water temperature can significantly affect the surrounding environment and social development, continuous monitoring of lake temperature changes is required. Traditional water monitoring methods are resource intensive and cannot simultaneously conduct extensive water monitoring. Remote sensing of temperature inversion has the advantages of all-weather, efficient, and large-scale real-time monitoring. Six Landsat 8 images from August to October in 2020 and 2021 were utilized to extract lake surface temperature (LST), and the variations in LST over the two years were analyzed to determine the impact of global climate anomalies on inland lakes. The results indicate that the LST in August and October 2021 was significantly higher than that in the same periods of the previous year, and the temperature difference in October reached 8 °C. In contrast to the overall normal distribution pattern of the water temperature in 2020, 2021 exhibited a relatively concentrated, unimodal distribution pattern. A trend analysis of the driving factors suggests that the LST of Poyang Lake is influenced by the global climate, and the artificial heat sources around the lake clearly alter the distribution characteristics of the LST simultaneously.