Quantitative response of cell growth and Tuber polysaccharides biosynthesis by medicinal mushroom Chinese truffle Tuber sinense to metal ion in culture medium

Tang, Ya‐Jie; Zhu, Lin; Liu, Rui-Sang; Li, Hongmei; Li, Dong‐Sheng; Mi, Zhiyuan

doi:10.1016/j.biortech.2008.02.006

Cited by 39 publications

(23 citation statements)

References 15 publications

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“…The dry cell weight, EPS production, and IPS production increased with increased initial sucrose concentration from 20 to 125 g/L, but higher sucrose concentration (i.e., 80 g/L) did not appear to enhance the effect. Thus, 80 g/L sucrose was considered the best initial level for submerged fermentation of T. sinense, and the maximum biomass (i.e., 22.81±1.03 g/L), EPS production (i.e., 2.33±0.22 g/L), and IPS production (i.e., 2.92±0.20 g/L) were enhanced by 82.5, 194.9, and 141.3 %, respectively, compared with that obtained from 20 g/L sucrose (Tang et al 2008a). …”

Section: Effect Of Carbon Sourcementioning

confidence: 86%

“…Lactose can be hydrolyzed into glucose and β-galactose by β-galactosidase, and glucose and β-galactose could be used for cellular growth of T. sinense. Further study showed the absence of β-galactosidase activity in the truffle fermentation system, which might explain why the growth of T. sinense was seriously inhibited when lactose was used as a carbon source (Tang et al 2008a). Thus, sucrose was viewed as the most favorable carbon source for truffle fermentation.…”

Section: Effect Of Carbon Sourcementioning

confidence: 98%

“…Sucrose was favorable for biomass (i.e., 14.97±0.08 g/L) and IPS production (i.e., 1.91±0.05 g/L). Lactose was best for EPS production (i.e., 1.61±0.40 g/L) but seriously inhibited biomass generation (i.e., 4.65±0.08 g/L) (Tang et al 2008a). Lactose can be hydrolyzed into glucose and β-galactose by β-galactosidase, and glucose and β-galactose could be used for cellular growth of T. sinense.…”

Section: Effect Of Carbon Sourcementioning

confidence: 99%

“…The highest EPS production (5.86±0.21 g/L) was achieved by the combination of 30 mM Mg 2+ , 5 mM K + , and 80 g/L sucrose (Tang et al 2008b). A complete enriched medium (i.e., 80 g/L sucrose, 10 g/L peptone, 30 g/L yeast extract, 5 mM K + , and 30 mM Mg 2+ ) was therefore proposed to further enhance the biomass and the production of EPS and IPS (Tang et al 2008a). However, only biomass was enhanced by the enriched medium, while the biosynthesis of truffle polysaccharides dramatically decreased.…”

Section: Development Of Fed-batch Fermentation Processmentioning

confidence: 99%

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Current progress on truffle submerged fermentation: a promising alternative to its fruiting bodies

Tang

Liu

2015

Appl Microbiol Biotechnol

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Truffle (Tuber spp.), also known as "underground gold," is popular in various cuisines because of its unique and characteristic aroma. Currently, truffle fruiting bodies are mostly obtained from nature and semi-artificial cultivation. However, the former source is scarce, and the latter is time-consuming, usually taking 4 to 12 years before harvest of the fruiting body. The truffle submerged fermentation process was first developed in Tang's lab as an alternative to its fruiting bodies. To the best of our knowledge, most reports of truffle submerged fermentation come from Tang's group. This review examines the current state of the truffle submerged fermentation process. First, the strategy to optimize the truffle submerged fermentation process is summarized; the final conditions yielded not only the highest reported truffle biomass but also the highest production of extracellular and intracellular polysaccharides. Second, the comparison of metabolites produced by truffle fermentation and fruiting bodies is presented, and the former were superior to the latter. Third, metabolites (i.e., volatile organic compounds, equivalent umami concentration, and sterol) derived from truffle fermentation could be regulated by fermentation process optimization. These findings indicated that submerged fermentation of truffles can be used for commercial production of biomass and metabolites as a promising alternative to generating its fruiting bodies in bioreactor.

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Section: Effect Of Carbon Sourcementioning

confidence: 86%

Section: Effect Of Carbon Sourcementioning

confidence: 98%

Section: Effect Of Carbon Sourcementioning

confidence: 99%

Section: Development Of Fed-batch Fermentation Processmentioning

confidence: 99%

See 2 more Smart Citations

Current progress on truffle submerged fermentation: a promising alternative to its fruiting bodies

Tang

Liu

2015

Appl Microbiol Biotechnol

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“…The potato dextrose agar slant and preparation of the preculture were previously described (24,33,34). Liquid seed medium consisted of glucose at 30 g/liter, NaNO 3 at 2 g/liter, K 2 HPO 4 at 1 g/liter, KCl at 0.5 g/liter, MgSO 4 ⅐ 7H 2 O at 0.5 g/liter, and FeSO 4 at 0.01 g/liter, and the pH was adjusted to 6.6 with 1.0 M HCl.…”

Section: Methodsmentioning

confidence: 99%

Novel Tandem Biotransformation Process for the Biosynthesis of a Novel Compound, 4-(2,3,5,6-Tetramethylpyrazine-1)-4′-Demethylepipodophyllotoxin

Tang

Zhao

2011

Appl Environ Microbiol

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According to the structure of podophyllotoxin and its structure-function relationship, a novel tandem biotransformation process was developed for the directional modification of the podophyllotoxin structure to directionally synthesize a novel compound, 4-(2,3,5,6-tetramethylpyrazine-1)-4-demethylepipodophyllotoxin (4-TMP-DMEP). In this novel tandem biotransformation process, the starting substrate of podophyllotoxin was biotransformed into 4-demethylepipodophyllotoxin (product 1) with the demethylation of the methoxyl group at the 4 position by Gibberella fujikuroi SH-f13, which was screened out from Shennongjia prime forest humus soil (Hubei, China). 4-Demethylepipodophyllotoxin (product 1) was then biotransformed into 4-demethylpodophyllotoxone (product 2) with the oxidation of the hydroxyl group at the 4 position by Alternaria alternata S-f6, which was screened out from the gathered Dysosma versipellis plants in the Wuhan Botanical Garden, Chinese Academy of Sciences. Finally, 4-demethylpodophyllotoxone (product 2) and ligustrazine were linked with a transamination reaction to synthesize the target product 4-TMP-DMEP (product 3) by Alternaria alternata S-f6. Compared with podophyllotoxin (i.e., a 50% effective concentration [EC 50 ] of 529 M), the EC 50 of 4-TMP-DMEP against the tumor cell line BGC-823 (i.e., 0.11 M) was significantly reduced by 5,199 times. Simultaneously, the EC 50 of 4-TMP-DMEP against the normal human proximal tubular epithelial cell line HK-2 (i.e., 0.40 M) was 66 times higher than that of podophyllotoxin (i.e., 0.006 M). Furthermore, compared with podophyllotoxin (i.e., log P ‫؍‬ 0.34), the water solubility of 4-TMP-DMEP (i.e., log P ‫؍‬ 0.66) was significantly enhanced by 94%. For the first time, the novel compound 4-TMP-DMEP with superior antitumor activity was directionally synthesized from podophyllotoxin by the novel tandem biotransformation process developed in this work.Podophyllotoxin (PTOX) is a naturally occurring aryltetralin lignan, and interest in it has been heightened by its potential antitumor activity (3, 16) but has failed to be used in human clinical trials because of unacceptable gastrointestinal toxic side effects (3). In order to overcome these limitations, numerous studies of Podophyllum lignans currently focus on the structural modification of the cycloparaffin (C ring) in the tetranap skeleton and the benzene with three methoxyl groups (E ring) to generate derivatives with superior pharmacological profiles (25). Aromatic heterocyclic compounds substituted with a carbon-nitrogen (CON) bond at the 4 position (14) may be a very effective pathway for improving the antitumor activity of PTOX, and most of these derivatives were more active than etoposide (1,5,7,8,15,20), which is a clinically important anticancer drug. Furthermore, previous investigations indicated that a PTOX derivative in which the para-position methoxyl group (4Ј-MeO) of PTOX was demethylated shows stronger anticancer activity and solubility than PTOX (29, 39). All of these analyses suggested that...

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Secondary Metabolites from Higher Fungi: Discovery, Bioactivity, and Bioproduction

Zhong

Xiao

2009

Biotechnology in China I

106

103

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Medicinal higher fungi such as Cordyceps sinensis and Ganoderma lucidum have been used as an alternative medicine remedy to promote health and longevity for people in China and other regions of the world since ancient times. Nowadays there is an increasing public interest in the secondary metabolites of those higher fungi for discovering new drugs or lead compounds. Current research in drug discovery from medicinal higher fungi involves a multifaceted approach combining mycological, biochemical, pharmacological, metabolic, biosynthetic and molecular techniques. In recent years, many new secondary metabolites from higher fungi have been isolated and are more likely to provide lead compounds for new drug discovery, which may include chemopreventive agents possessing the bioactivity of immunomodulatory, anticancer, etc. However, numerous challenges of secondary metabolites from higher fungi are encountered including bioseparation, identification, biosynthetic metabolism, and screening model issues, etc. Commercial production of secondary metabolites from medicinal mushrooms is still limited mainly due to less information about secondary metabolism and its regulation. Strategies for enhancing secondary metabolite production by medicinal mushroom fermentation include two-stage cultivation combining liquid fermentation and static culture, two-stage dissolved oxygen control, etc. Purification of bioactive secondary metabolites, such as ganoderic acids from G. lucidum, is also very important to pharmacological study and future pharmaceutical application. This review outlines typical examples of the discovery, bioactivity, and bioproduction of secondary metabolites of higher fungi origin.

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Quantitative response of cell growth and Tuber polysaccharides biosynthesis by medicinal mushroom Chinese truffle Tuber sinense to metal ion in culture medium

Cited by 39 publications

References 15 publications

Current progress on truffle submerged fermentation: a promising alternative to its fruiting bodies

Current progress on truffle submerged fermentation: a promising alternative to its fruiting bodies

Novel Tandem Biotransformation Process for the Biosynthesis of a Novel Compound, 4-(2,3,5,6-Tetramethylpyrazine-1)-4′-Demethylepipodophyllotoxin

Secondary Metabolites from Higher Fungi: Discovery, Bioactivity, and Bioproduction

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