Quantitative RP-HPLC Determination of Some Aldehydes and Hydroxyaldehydes as Their 2,4-Dinitrophenylhydrazone Derivatives

Koivusalmi, Eija; Haatainen, E.; Root, Andrew

doi:10.1021/ac980699f

Cited by 43 publications

(30 citation statements)

References 31 publications

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“…Because the run time for binary mixtures with large water content (e.g., 30% ACN (exp 1: AW = 30:70)) was extended significantly (over approximately 500 min), the actual evaluation of our data was made after excluding exp No. was commonly observed in several prior studies (e.g., Koivusalmi et al 1999;Ochs et al 2010). …”

Section: Resultssupporting

confidence: 76%

Effect of HPLC binary mobile phase composition on the analysis of carbonyls

Kim

2010

Environ Monit Assess

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The relative performance of the binary mobile phase in the high-performance liquid chromatography analysis of carbonyl compounds (CCs) was tested using the liquid-phase standards containing 15 aldehyde/ketone-DNPH mixture. The Hichrome column was employed for the analysis of CCs at a flow rate of 1.5 mL min( - 1). The binary mobile phases prepared using both acetonitrile/water (AW) and a possible alternative of methanol:water (MW) mixture were examined by their calibration results. The data derived from these two binary phases were then evaluated in terms of three key variables (i.e., resolution, relative sensitivity, and retention time). The relative water content (or the water to organic solvent ratio (W/A) or (W/M)) of the binary phase was found as the key variable for the performance. The results indicate that the optimal resolution of AW combination was attained consistently for most composition, while MW generally suffered from overpressure problem. The changes of water content in the AW mixture led to the changes of all three variables in the quantitative analysis of CCs. The obtained results confirm that the AW mixture should be the optimal elutant for the CC analysis, as other simple binary compositions like MW are limited in many respects.

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Section: Resultssupporting

confidence: 76%

Effect of HPLC binary mobile phase composition on the analysis of carbonyls

Kim

2010

Environ Monit Assess

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“…Typical UV spectra of hydroxy aldehyde ( ) with one strong and one weak absorption maximum, hydroxy acid (--) with two strong absorption maxima, unsaturated acid (---), carboxylic acid ( .... -), unsaturated aldehydes AEA (----) and 2-ethyl-2-hexenal (---% about 4-10 mg L l. The quantification limits of aliphatic aldehydes are about 50 times, and hydroxy aldehydes about 150 200 times higher than in the DNPH method [14]. Thus, the DNPH method should be used if low concentrations of aldehydes are to be determined, and the HPLC method used to analyze all the other compounds except formaldehyde.…”

Section: Figurementioning

confidence: 96%

“…To test the reliability of the developed method a BHPAL and a BHBAL sample were analyzed also by a DNPH-HPLC method [14]. The BHBAL results showed a good agreement and the BHPAL results a poorer agreement with each other, as presented in Table I.…”

Section: Quantitative Analysis and Identification Of The Componentsmentioning

confidence: 99%

“…The different forms of 2,2-dimethyl-3-hydroxypropionaldehyde (DHPAL) have been determined in reaction mixtures by GC as trimethylsilyl derivatives, and the dimeric form was shifted quantitatively to monomeric DHPAL by oxime/trimethylsilyl-derivatization [13]. Aldehydes and hydroxy aldehydes have been determined as their 2,4-dinitrophenylhydratzine (DNPH) derivatives by reversed phase HPLC analysis [14]. The acids, hydroxy acids, hydroxy aldehydes, and polyols can be analyzed by HPLC without derivatization.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the compounds in the synthesis mixtures of hydroxy acids by a gradient programmed RP-HPLC with UV/vis and RI detectors

et al. 2000

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KeyWordsColumn liquid chromatography Nuclear magnetic resonance spectroscopy Hydroxy carboxylic acids SummaryA gradient programmed reversed phase high-performance liquid chromatographic (RP-HPLC) method is described for the quantitative determination of some aldehydes, hydroxy aldehydes, unsaturated aldehydes, hydroxy carboxylic acids, carboxylic acids, alcohols and polyols using an ultraviolet and a refractive index detector in series. The sample matrices are synthesis mixtures of hydroxy carboxylic acids. The structures of the hydroxy aldehyde intermediates are determined by 13C NMR and the influence of sample preparation on analysis results is discussed. The limits of detection and the precision of the method are evaluated.

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“…In most cases, due to the physicochemical properties of this group of compounds, analytical procedures include derivatization step. Frequently used as a derivatization reagents are such derivatives of hydrazine as: 2,4-dinitrophenylhydrazine (DNPH) [5,12,17,21], 2,4,6-trichlorophenylhydrazine (TCPH) [20], 2,3,4,5,6-pentafl uorophenylhydrazine (PFPH) [14], 5-(dimethylamino)-naphthalene-1-sulfon-hydrazide (DNSH) [19], 3-methylbenzothiazole-2-hydrazine (MBTH) [11], 4-(N.N-dimethylaminosulfonyl)-7-N-methylhydrazino-2,1,3-benzoxadiazole (MDBDH) [9], 5-amino-4-sulfanylphthalhydrazide (ASPH) [8], hydroxylamine derivatives (O-benzylhydroxylamine (BOA) [10], o- (2,3,4,5,6-pentafl uorobenzyl)-hydroxylamine (PFBHA) [15], Hantzscha reagents (1,3-cykloheksadion (CHD), 5,5-dimethyl-1,3-cykloheksadion (Dimedon), 4-amino--3-penten-2-one (Fluoral P), acetylacetone, acetoacetanilid or 2-aminoethanethiol (cysteamine), 3-amonofl uoroethan [6,22]. DNPH is the commonly used derivatization agent for high performance liquid chromatography (HPLC), whereas PFBHA is usually used for gas chromatography.…”

Section: Archives Of Environmental Protection Vol 40 No 2 Pp 21 -3mentioning

confidence: 99%

Determination of Aldehydes in Wet Deposition

Czaplicka¹,

Jaworek²,

Wochnik³

2014

Archives of Environmental Protection

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Abstract:The paper presents two sample preparation procedures for the determination of aldehydes in wet deposition. In both cases the 2,4-dinitrophenylhydrazine derivatization and solid phase extraction were applied. The derivatization in method A was applied before the extraction, the extraction in method B was carried out with simultaneous derivatisation. Accuracy of both methods was evaluated on the basis of the analysis of aqueous solutions of selected carbonyl compounds. Both methods were characterized by good recovery, however, due to the precision of the method expressed as RSD for testing of environmental samples the method B was used.The analysis of environmental samples showed signifi cant differences in the concentrations of aldehydes in wet deposition, depending on the location of the sampling point. In the case of samples taken from agricultural areas the predominant aldehydes were formaldehyde and acetaldehyde. Formaldehyde was from 31% to 47% of the determined compounds. While in samples collected near a traffi c source, in the deposition acrolein was determined at the levels from 62% to 64% of the identifi ed compounds.

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Quantitative RP-HPLC Determination of Some Aldehydes and Hydroxyaldehydes as Their 2,4-Dinitrophenylhydrazone Derivatives

Cited by 43 publications

References 31 publications

Effect of HPLC binary mobile phase composition on the analysis of carbonyls

Effect of HPLC binary mobile phase composition on the analysis of carbonyls

Analysis of the compounds in the synthesis mixtures of hydroxy acids by a gradient programmed RP-HPLC with UV/vis and RI detectors

Determination of Aldehydes in Wet Deposition

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