Quantitative separation of antioxidant pigments in purple sea urchin shells using a reversed-phase high performance liquid chromatography

Kuwahara, Rui; Hatate, Hideo; Chikami, Atsuto; Murata, Hisashi; Kijidani, Yoshio

doi:10.1016/j.lwt.2010.03.005

Cited by 26 publications

(29 citation statements)

References 13 publications

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“…Similar results were obtained by [16], suggesting that PHNQ pigments are highly concentrated in the shells. This was confirmed by the orange to the brown-like color of the extracts.…”

Section: Shell Extractsupporting

confidence: 84%

Bioactive potential of Diadema sp. from the South East Coast of Mauritius

2017

J App Biol Biotech

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As a consequence of the alarming rise in antimicrobial resistance, this study aimed at evaluating the bioactive potential of sea urchin extract, Diadema sp. Two different tissues; shells and gonads were used and two drying methods were compared; freeze-drying (FD) and non-FD (NFD). Statistically, significant interaction was observed between the drying methods and solvents used. Compared to the gonadal tissue, NFD methanolic shell extract reported higher extraction yield, 9.13 ± 0.87%. Phenolic content assessed using the Folin-Ciocalteu method was higher in NFD gonadal extract, 688.1 mg gallic acid equivalents/g distilled water. Promising antimicrobial activity was recorded using agar disc diffusion and results confirmed using minimum inhibitory concentration. Among the bacteria used, Escherichia coli and Staphylococcus aureus were more vulnerable, reporting inhibition zone of 16.3 ± 1.37 mm and 17.1 ± 0.23 mm using NFDSM and FDGM extract, respectively. DPPH antioxidant assay showed NFDSM having better activity than the positive control, half maximal inhibitory concentration of 3.77 µg/ml. No significant larvicidal activity was recorded. Diadema sp. are a potential source of novel antimicrobial compounds and suggest its possible use as natural food-grade antioxidant. This study also shows NFD method and methanol as the recommended solvent for maximal bioactivity.

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“…Similar results were obtained by [16], suggesting that PHNQ pigments are highly concentrated in the shells. This was confirmed by the orange to the brown-like color of the extracts.…”

Section: Shell Extractsupporting

confidence: 84%

Bioactive potential of Diadema sp. from the South East Coast of Mauritius

2017

J App Biol Biotech

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“…The obtained results showed that total phenols of purple sea urchin E. mathaei tissue extracts are similar to those of gonads (53 mg GAE/100g) and digestive tracts (123 mg GAE/100 g) of green sea urchin centrotus droebachiensis and shells (1.5 mg GAE/g) of red-brown sea urchin Psammechinus miliaris (18). Phenolic compounds may account for antioxidant properties such as reducing power (5), which are caused by hydroxyl groups of these molecules (19,20).…”

Section: Discussionmentioning

confidence: 99%

Determination of In Vitro Antioxidant Properties, Anti-inflammatory Effects and A-Amylase Inhibition of Purple Sea Urchin Extract of Echinometra mathaei from the Persian Gulf

Soleimani

Moein

Yousefzadi

et al. 2016

Jundishapur J Nat Pharm Prod

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“…For example, earlier ndings by Anderson and co-authors did not describe echinochrome A as being present in E. mathaei, H. crassispina, P. milliaris, T. gratilla, 7 but recent reports have found this compound in all of these species. 6,28,35,61 It was likely more challenging to identify spinochromes earlier because of a lack of modern instrumentation such as LC-MS and high-resolution NMR. It is also well known that the composition of secondary metabolites in marine organisms may differ signicantly due to geographic, ecological, seasonal, gender and other variables.…”

Section: Family Schizasteridaementioning

confidence: 99%

Naphthoquinones of the spinochrome class: occurrence, isolation, biosynthesis and biomedical applications

et al. 2018

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Quinones are widespread in nature and have been found in plants, fungi and bacteria, as well as in members of the animal kingdom. More than forty closely related naphthoquinones have been found in echinoderms, mainly in sea urchins but occasionally in brittle stars, sea stars and starfish. This review aims to examine controversial issues on the chemistry, biosynthesis, functions, stability and application aspects of the spinochrome class, a prominent group of secondary metabolites found in sea urchins. The emphasis of this review is on the isolation and structure of these compounds, together with evaluation of their relevant biological activities, source organisms, the location of origin and methods used for isolation and identification. In addition, the studies of their biosynthesis and ecological function, stability and chemical synthesis have been highlighted. This review aims to establish a focus for future spinochrome research and its potential for benefiting human health and well-being. Scheme 2 Synthesis of spinochrome E (6) as described by Borisova and Anufriev. 94 Reagents and conditions: (i) (EtO) 3 CH, reflux; (ii) NaNO 2 , EtOH, H 2 O, heating; (iii) Na 2 S 2 O 4 , H 2 O; (iv) AlCl 3 , PhNO 2 , heating, then 5% HCl, heating. Scheme 1 Synthesis of spinochromes as described by Singh et al. 90 Reagents and conditions: (i) AlCl 3 -NaCl, 195 C; (ii) MeONa, MeOH, reflux; (iii) conc. HBr, reflux.This journal is

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Quantitative separation of antioxidant pigments in purple sea urchin shells using a reversed-phase high performance liquid chromatography

Cited by 26 publications

References 13 publications

Bioactive potential of Diadema sp. from the South East Coast of Mauritius

Bioactive potential of Diadema sp. from the South East Coast of Mauritius

Determination of In Vitro Antioxidant Properties, Anti-inflammatory Effects and A-Amylase Inhibition of Purple Sea Urchin Extract of Echinometra mathaei from the Persian Gulf

Naphthoquinones of the spinochrome class: occurrence, isolation, biosynthesis and biomedical applications

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