Quantitative Significance of Plasma Albumin Metabolism in the Whole Body Protein Turnover in Rats

Kawabata, Hiroaki; Noguchi, Tadashi; Naitoi, Hiroshi

doi:10.1080/00021369.1983.10865946

Cited by 1 publication

(1 citation statement)

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“…Thus, the flux of leucine calculated from the data on skin mucus, 9 to 10 days after the beginning of mucus and plasma collection, may be over estimated by about 20%. The assessment of recycling of labelled amino acids from the pattern of changes of the radioactivity in the plasma protein is partially justified by their large contribution to whole body protein turnover (Kawabata et al 1983).…”

Section: Validity Of the Methodsmentioning

confidence: 99%

Measurement of plasma leucine flux in rainbow trout (Salmo gairneri R.) using osmotic pump. Preliminary investigations on influence of diet

Fauconneau¹,

Tesseraud²

1990

Fish Physiol Biochem

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A method for the direct measurement of plasma amino acid flux, in rainbow trout, using the continuous infusion of L-[U-(14)C]-leucine with ALZET mini-osmotic pumps implanted into the peritoneal cavity, was developed. The fish were fed successively on three different diets (a commercial control diet, a semi-purified diet and a purified diet) during the 4 weeks of experiment. The amounts of radioactivity in the free pool and the protein of both the plasma and skin mucus were measured in these fish. The total flux of leucine was calculated either from the specific activity of leucine in the plasma (61.8 mg leucine. 100 g(-1).d(-1)) or from the amounts of labelled and unlabelled leucine flowing into the skin mucus protein (61.5 mg leucine. 100g(-1).day(-1)). The total plasma flux was not affected by changes in the diet. The contributions of total leucine oxidation and whole body protein turnover to plasma leucine flux (80% and 20% respectively) were estimated in fish fed the semi-purified diet.

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Section: Validity Of the Methodsmentioning

confidence: 99%