Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering

Graewert, Melissa A.; Wilhelmy, Christoph; Bacic, Tijana; Schumacher, Jens; Blanchet, Clement; Meier, Florian; Drexel, Roland; Welz, Roland; Kolb, Bastian; Bartels, Kim; Nawroth, Thomas; Klein, Thorsten; Svergun, Dmitri; Langguth, Peter; Haas, Heinrich

doi:10.1038/s41598-023-42274-z

Cited by 13 publications

(1 citation statement)

References 48 publications

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“…Dynamic light scattering DLS [68,69] Electron microscopy EM [70,71] Energy dispersive X-ray spectroscopy EDS [72,73] Environmental scanning electron microscopy ESEM [74,75] Field-flow fractionation FFF [76,77] Flame atomic absorption spectrometry F-AAS [78,79] Graphite furnace atomic absorption spectrometry GF-AAS [80,81] High-performance liquid chromatography HPLC [42,82] Hydrodynamic chromatography HDC [83,84] Infrared spectroscopy IR [85,86] Inductively coupled plasma mass spectrometry ICP-MS [87,88] Microscopical analysis plays a pivotal role in characterizing nanoparticles within coatings, offering valuable insights into their purity and composition. Utilizing an electron microscope, analytical electron microscopy (AEM) facilitates the comprehensive characterization of nanoparticles.…”

Section: Methods Name Abbreviation Referencesmentioning

confidence: 99%

Characterization of Nanoparticles in Antimicrobial Coatings for Medical Applications—A Review

Rezić,

Meštrović

2023

Coatings

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This review discusses relevant topics concerning the understanding of the characterization of antimicrobial coatings due to powerful antimicrobial nanoparticles in their composition. These coatings are utilized in the surface modification of yarns and materials designed for use in medical and dental applications. Various physical and chemical methods are employed to create these coatings, ensuring the development of efficient, homogeneous, and uniform layers on diverse surfaces and materials. The primary objective is to confer antimicrobial and/or antiviral properties upon these materials. For these coatings to be effective, they must incorporate active compounds that can combat a wide array of microorganisms, including those that have developed resistance to antibiotics. Examples of such active compounds include metallic nanoparticles such as silver, copper, and gold, as well as nanoparticles of metal oxides such as zinc, titanium, and aluminum. Upon the application of these coatings to medical materials, extensive testing and characterization procedures are undertaken, which will be thoroughly detailed in this review. It is crucial to emphasize that the absence of proper characterization and testing of nanoparticles in antimicrobial coatings could lead to the absence of standards, norms, or procedures necessary to safeguard human health and the environment. Despite their widespread application in the medical field, concerns have been raised regarding the potential toxicity of nanoparticles to living organisms. Consequently, this paper provides a comprehensive overview of the current state-of-the-art methodologies for characterizing nanoparticles in antimicrobial coatings, specifically focusing on materials with varying roughness and structures. Additionally, it outlines the issues associated with the potential accumulation of antimicrobial nanoparticles within the human body.

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Section: Methods Name Abbreviation Referencesmentioning

confidence: 99%

Characterization of Nanoparticles in Antimicrobial Coatings for Medical Applications—A Review

Rezić,

Meštrović

2023

Coatings

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On the Influence of Fabrication Methods and Materials for mRNA‐LNP Production: From Size and Morphology to Internal Structure and mRNA Delivery Performance In Vitro and In Vivo

Bi,

Wilhelmy,

Unthan

et al. 2024

Adv Healthcare Materials

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Lipid nanoparticle (LNP) remains the most advanced platform for messenger RNA (mRNA) delivery. To date, mRNA LNPs synthesis is mostly performed by mixing lipids and mRNA with microfluidics. In this study, a cost‐effective microfluidic setup for synthesizing mRNA LNPs is developed. It allows to fine‐tune the LNPs characteristics without compromising LNP properties. It is compared with a commercial device (NanoAssemblr) and ethanol injection and the influence of manufacturing conditions on the performance of mRNA LNPs is investigated. LNPs prepared by ethanol injection exhibit broader size distributions and more inhomogeneous internal structure (e.g., bleb‐like substructures), while other LNPs show uniform structure with dense cores. Small angel X‐ray scattering (SAXS) data indicate a tighter interaction between mRNA and lipids within LNPs synthesized by custom device, compared to LNPs produced by NanoAssemblr. Interestingly, the better transfection efficiency of polysarcosine (pSar)‐modified LNPs correlates with a higher surface roughness than that of PEGylated ones. The manufacturing approach, however, shows modest influence on mRNA expression in vivo. In summary, the home‐developed cost‐effective microfluidic device can synthesize LNPs and represents a potent alternative to NanoAssemblr. The preparation methods show notable effect on LNPs’ structure but a minor influence on mRNA delivery in vitro and in vivo.

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Advances in the design and delivery of RNA vaccines for infectious diseases

Lokras,

Bobak,

Baghel

et al. 2024

Advanced Drug Delivery Reviews

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Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering

Cited by 13 publications

References 48 publications

Characterization of Nanoparticles in Antimicrobial Coatings for Medical Applications—A Review

Characterization of Nanoparticles in Antimicrobial Coatings for Medical Applications—A Review

On the Influence of Fabrication Methods and Materials for mRNA‐LNP Production: From Size and Morphology to Internal Structure and mRNA Delivery Performance In Vitro and In Vivo

Advances in the design and delivery of RNA vaccines for infectious diseases

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