Quantitative Studies by Electron Microscopy on the Sex-Difference and the Change during the Oestrous Cycle in the Mouse Anterior Pituitary

Sasaki, Fumihiko

doi:10.1679/aohc1950.37.41

Cited by 23 publications

(13 citation statements)

References 47 publications

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“…In adult mice, there is a clear sex difference in the population of GH cells in the adenohypophysis; they are more abundant in males (Sasaki, 1974;Sasaki and Sano, 1980). GH levels both in the pituitary (Yanai and Nagasawa, 1968; and in serum ) are higher in male mice than in the females.…”

Section: Discussionmentioning

confidence: 99%

“…Mice that showed a normal estrous cycle were decapitated on the day of diestrus. To ensure that the electron micrographs were representative of all parts of the anterior pituitary gland, stereological morphometry for electron microscopy was used as described elsewhere (Sasaki, 1974). All methods for electron microscopy used in this study are described in the 1974 paper and in another (Sasaki et al, 1979).…”

Section: Methodsmentioning

confidence: 99%

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Growth Hormone Cells in the Adenohypophysis Compared in Mice with High and Low Incidence of Mammary Tumors by Stereological Morphometry with Electron Microscopy

Sasaki

1988

Okajimas Folia Anatomica Japonica

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Summary: Growth hormone (GH) cells in the adenohypophysis of adult female mice of the C3H/He strain, with a high incidence of mammary tumors, and of the C57BL/6 strain, with a low incidence of such tumors, were compared by stereological morphometry with electron microscopy. There was no significant difference in the population of GH cells between the two strains of mice. The size of GH cells was significantly larger in the C3H/He mice (P<0.01). In ultrastructural observations by electron microscopy, GH cells in the C3H/He mice contained more secretory granules and better-developed cell organelles than those in the C57BL/6 mice. The body weight was significantly greater in the C3H/He strain than in the C57BL/6 one (P<0.001). These results suggested that GH cells in the C3H/He mice may have higher GH synthetic activity than those in the C57BL/6 strain.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Growth Hormone Cells in the Adenohypophysis Compared in Mice with High and Low Incidence of Mammary Tumors by Stereological Morphometry with Electron Microscopy

Sasaki

1988

Okajimas Folia Anatomica Japonica

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“…Moreover, the state of activity in terms of GH synthesis and storage versus GH release may affect cell granule content, and diurnal variations and strain differences may also affect these measurements in developing animals, as in adults [Yamamoto et al. 1970: Sinha el al.. 1974Sasaki andSano. 1980.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Aspects of Growth Hormone Cell Maturation in the Normal and Little Mutant Mouse

Wilson¹,

Wyatt²,

Gadler³

et al. 1988

Cells Tissues Organs

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Growth hormone (GH) cells were analyzed by means of ultrastructural morphometry in the pars distalis of pituitary glands from male adult and immature normal (C57BL) and homozygous little (lit/lit) mutant mice. Thin sections were exposed to anti-GH serum and processed immunocytochemically with the colloidal-gold technique. In the pars distalis of adult lit/lit mice, the mean volume density of GH cells/total tissue was 24% of the normal value, granules/GH cells was 58% of normal, and granules/total tissue was only 12% of normal. Deficits in all of these parameters likewise occurred in immature glands, though to a lesser extent than in the adults.The results indicate that the GH deficiency in this mutant reflects quantitative deficits in both the secretory granule content of GH cells, as well as the GH cell content of the gland, with the latter being the more severely affected.

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“…Each hemipituitary gland was diced into 6 pieces (Sasaki, 1974), and these were fixed in 0504 for 1 h at 4°C. The blocks were dehydrated in a graded series of ethanol solutions and embedded in Epon (Coulter, 1967) for 5 days at 60°C.…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical Study of Adenohypophysial Gonadotropes in the Musk Shrew, Suncus murinus L. (Insectivora)

Iwama

Nakano

Hasegawa

1990

Okajimas Folia Anatomica Japonica

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Quantitative Studies by Electron Microscopy on the Sex-Difference and the Change during the Oestrous Cycle in the Mouse Anterior Pituitary

Cited by 23 publications

References 47 publications

Growth Hormone Cells in the Adenohypophysis Compared in Mice with High and Low Incidence of Mammary Tumors by Stereological Morphometry with Electron Microscopy

Growth Hormone Cells in the Adenohypophysis Compared in Mice with High and Low Incidence of Mammary Tumors by Stereological Morphometry with Electron Microscopy

Quantitative Aspects of Growth Hormone Cell Maturation in the Normal and Little Mutant Mouse

Immunohistochemical Study of Adenohypophysial Gonadotropes in the Musk Shrew, Suncus murinus L. (Insectivora)

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