Quantitative studies of coccidial development in miniature cultures of animal cells

Purification of Eimeria Sporozoites by DE‐52 Anion Exchange Chromatography

“…T sporulated oocysts are ground in a tissue grinder to release sporocysts (1 l), which are subsequently treated with a sodium taurodeoxycholate-trypsin excysting solution to release the sporozoites (12). During this process, large amounts of parasite debris (oocysts, oocyst walls, sporocysts, and sporocyst shells) are generated resulting in a crude, impure sporozoite suspension which is cytotoxic in cell cultures.…”