Quantitative studies of estrogen transfer between parabiotic rats in relation to the effect of p p′ dihydroxy‐a‐b‐diethylstilbene on the secretion of the gonadotropic complex

Biddulph, Clyde; Meyer, Roland K.; Gumbreck, Laurence G.

doi:10.1002/jez.1400880103

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…The amplification effect could account for prolonged biologic action despite a short plasma half-life. Our results with exogenous parathyroid hormone and endogenous parathyroid hormone are in accord with the known characteristic of other protein hormones, the hypo¬ physeal gonadotrophins, of stimulating ovarian growth and steroid secretion across the parabiotic union when castrated or ovariectomized rats are joined to partners with intact gonads (Rice 8c Segaloff 1966è;Biddulph et al 1941). Vitamin D.< produced severe hypercalcaemia in the treated parabionts but little change in the serum calcium levels of the untreated TPX parabionts (Figs.…”

Section: Discussionsupporting

confidence: 89%

“…4 and 5). These results are in accord with the inability of other steroid hormones, the sex steroids, to stimulate accessory sex organs across the para¬ biotic union (Rice 8c Segaloff 1966è;Biddulph et al 1941). These data are difficult to reconcile at this time with recent studies of vitamin D metabolism suggesting a long plasma half-life for these compounds.…”

Section: Discussionsupporting

confidence: 84%

“…Peptide hor¬ mones, such as the hypophyseal gonadotrophins, are known to stimulate target organs across the parabiotic union (Rice 8c Segaloff 1966Ò). Certain steroid hormones, such as the oestrogens, do not appear to manifest an action on the target organ across the parabiotic junction (Rice 8c Segaloff 1966 ;Biddulph et al 1941). We would like to report the results of a comparative study in which the parabiosis model was used to characterize the hypercalcaemic states produced by parathyroid hormone, used as the model peptide, and exogenous vitamin D,j, used as the model steroid.…”

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confidence: 99%

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Hypercalcaemia of Neoplasia, Parathyroid Hormone and Vitamin D: Studies in Parabiosis

Ponthier

Rice

1974

Acta Endocrinologica

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A localized transplantable Leydig cell tumour of the Fischer rat regularly produces lethal hypercalcaemia in thyroparathyroidectomized and intact hosts. The parabiosis model was used to explore and compare the hypercalcaemia producing action of this tumour, parathyroid hormone, and vitamin D. Administration of exogenous parathyroid hormone (10 and 20 units) to one thyroparathyroidectomized parabiont rat increased the serum calcium in both parabionts within 24 hours. Endogenous parathyroid hormone, studied by producing parabiosis between thyroparathyroidectomized rats and intact rats, raised the serum calcium of the thyroparathyroidectomized parabiont within 7 days in parabiosis, suggesting that endogenous parathyroid hormone is effective across the parabiotic union. Vitamin D3 (100 000 and 150 000 units) given to one parabiont of a thyroparathyroidectomized pair produced hypercalcaemia only in the injected parabionts. When the hypercalcaemia producing tumour was grown in thyroparathyroidectomized pairs for 3\p=n-\6 weeks, severe hypercalcaemia and death occurred only in the tumour bearing parabiont. The serum calcium of the non-tumour parabiont did rise over a period of 3\p=n-\6weeks and was compatible with an approach to calcium

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 84%

mentioning

confidence: 99%

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Hypercalcaemia of Neoplasia, Parathyroid Hormone and Vitamin D: Studies in Parabiosis

Ponthier

Rice

1974

Acta Endocrinologica

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“…Sections were cut at 8 µ and stained with Mallory's triple stain. The presence of India ink verified the age of the corpus 218 Guy H. Kiracofe and Harold G. Spies luteum under investigation since circulating oestrogens ofeither parabiont could reach levels sufficient to confuse the vaginal smear of the experimental parabiont (Biddulph, Meyer & Gumbreck, 1941). Fifteen rats of the same age and strain as described in the four parabiosis groups were hysterectomized following cervical stimulation at early oestrus and divided into three equal groups to test for evidence of a uterine luteolysin.…”

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confidence: 99%

Effect of Parabiosis or Uterine Extracts on Pseudopregnancy in Hysterectomized Rats

Kiracofe¹,

Spies²

1966

Reproduction

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Regression of the corpora lutea was hastened in hysterectomized-pseudopregnant rats by parabiotic union with ovariectomized partners. Hysterectomized-pseudopregnant rats paired either with hysterectomized-pseudopregnant or with normal cycling partners had periods of pseudopregnancy similar in length to those in unpaired hysterectomized-pseudopregnant control females. Injections of ether-soluble extracts or of lyophilized homogenates of oestrous rat uteri did not shorten pseudopregnancy in hysterectomized rats.The technique of parabiosis (Bunster & Meyer, 1933) was employed to test for presence of a luteolysin from normal or ovariectomized rats that could cross the parabiotic union and influence the life-span of corpora lutea in pseudopregnant or hysterectomized-pseudopregnant partners. Sprague-Dawley females, 60 to 80 days old, were paired as follows: In Group 1, rats ovari¬ ectomized in di-oestrus were combined 4 or 5 days later with intact pseudo¬ pregnant ones which were in oestrus (Day 1) at the time of the operation; in Group 2, intact rats in oestrus were combined with hysterectomized-pseudo¬ pregnant ones on Day 4 or 5; in Group 3, pseudopregnant females hysterecto¬ mized immediately before parabiosis on Days 3, 4 or 5 were combined with rats ovariectomized 4 or 5 days previously; and in Group4, both pseudopregnant partners were hysterectomized and paired on Day 5. Pseudopregnancy was induced by cervical stimulation with a glass rod at late pro-oestrus or early oestrus. Rats showing vaginal cornification before Day 7 were discarded. Several of the largest corpora lutea in the hysterectomized-and intact-pseudopregnant partners were marked with India ink before joining, using the method described by Spies, Gier & Wheat (1964). Most rats were killed on the day after the first vaginal cornification following pairing or at the expected end of pseudopregnancy for the experimental parabiont; however, in a few cases repeated vaginal smears were followed. At autopsy ovaries were fixed in Bouin's fluid for histological study. Sections were cut at 8 µ and stained with Mallory's triple stain. The presence of India ink verified the age of the corpus * Present address :

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Commentary on and reprint of Reissmann KR, Studies on the mechanism of erythropoietin stimulation in parabiotic rats during hypoxia, in Blood (1950) 5:372–380

2000

Hematology

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Quantitative studies of estrogen transfer between parabiotic rats in relation to the effect of p p′ dihydroxy‐a‐b‐diethylstilbene on the secretion of the gonadotropic complex

Cited by 5 publications

References 10 publications

Hypercalcaemia of Neoplasia, Parathyroid Hormone and Vitamin D: Studies in Parabiosis

Hypercalcaemia of Neoplasia, Parathyroid Hormone and Vitamin D: Studies in Parabiosis

Effect of Parabiosis or Uterine Extracts on Pseudopregnancy in Hysterectomized Rats

Commentary on and reprint of Reissmann KR, Studies on the mechanism of erythropoietin stimulation in parabiotic rats during hypoxia, in Blood (1950) 5:372–380

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