Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments

Furuta, Takahisa; Kaneko, Eizō; Suzuki, Michihiro; Arai, Hajime; Futami, Hajime

doi:10.1128/jcm.34.10.2421-2425.1996

Cited by 34 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously reported a new method for quantification of H. pylori in gastric mucus by cPCR. 12 In the present study, we used this method to test for H. pylori in patients with duodenal ulcer treated with lansoprazole alone or lansoprazole and amoxycillin. Most patients treated with these drugs were negative when tested by RUT, histology, and culture, but returned to being H. pylori positive following cessation of treatment.…”

Section: Discussionmentioning

confidence: 99%

Amount of Helicobacter pylori in gastric mucus during anti‐H. pylori treatment

Furuta

Takashima

Futami

et al. 1998

J of Gastro and Hepatol

View full text Add to dashboard Cite

Helicobacter pylori is present in infected patients not only on the gastric epithelial cell surface but also in gastric mucus. We developed a competitive polymerase chain reaction (cPCR) method for quantitative measurement of H. pylori in gastric mucus. The aim of this study was to determine the number of H. pylori in gastric mucus before and after anti-H. pylori treatment. Patients with duodenal ulcer were treated with lansoprazole alone (n = 11) or lansoprazole and amoxycillin (n = 12). The amount of H. pylori in gastric mucus was measured over time by a cPCR assay. Helicobacter pylori infection was also tested for using histology, culture, and the rapid urease test (RUT). Although most patients treated with lansoprazole alone had become H. pylori-negative by the end of treatment when tested by histology, RUT, and culture, a large number of H. pylori organisms were found in the gastric mucus at that time by cPCR. These patients returned to being H. pylori positive 1 to 12 months later on the basis of histology, RUT, and culture. However, cPCR results indicated eradication of H. pylori by the end of treatment in eight of the 12 patients treated with lansoprazole and amoxicillin, and these patients remained H. pylori negative on histology, RUT, culture, and cPCR 1 to 12 months later. Testing for H. pylori in gastric mucus is thus useful for precise determination of the success or failure of H. pylori eradication therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Amount of Helicobacter pylori in gastric mucus during anti‐H. pylori treatment

Furuta

Takashima

Futami

et al. 1998

J of Gastro and Hepatol

View full text Add to dashboard Cite

show abstract

“…Other 31 biopsies of gastric cancer were not amplified of the desired 16S rDNA gene and showed the tissues negative for HP. The small size of gastric biopsy specimen or minor colonisation of pathogens may results of colonised and non-colonised tissues [24] .…”

Section: Discussionmentioning

confidence: 99%

Detection of HER2 expression and its structural alterations in gastric cancer tissues infected with cagA+H. pylori

Srivastava¹,

Singh

2020

Preprint

View full text Add to dashboard Cite

Background: Helicobacter pylori (HP) cagA is the causing agent for development of gastric cancer (GC). H. pylori also involves to trigger the EGFR (epidermal growth factor receptor) expression in gastric cancer cells. However, the prognostic relation of cagA with HER2 status in GC was not well understood. Objective:The main aim of this study was to investigate the link of HER2 expression with CagA+ H. pylori in GC tissues. Materials and Methods:The study was performed on 85 GC tissues of GC patients.The specific primers of 16S rDNA and cagA for PCR amplification were used. For investigation of HER2 status in GC tissues, immunohistochemistry and PCR amplification were performed. In silico study was performed for the investigation of interactive potential of HER2 with CagA protein. Results: PCR amplified the 54 (63.52 %) of 85 GC tissues for HP that showed 34 (62.96 %) cagA+ HP. Immunohistochemistry of tissues revealed 57 (67.05 %) diffuse and 28 (32.94 %) intestinal type cancer. Of 85 cases, 21 GC tissues scored (2 + or 3 +) for positive HER2 expression and score (0 or 1 +) of 64 (75.29 %) showed negative. Of 21 HER2 + GC tissue, 15 biopsies had cagA+ HP and 2 were negative. PCR amplified single amplicon in 17 (20 %) CagA+ tissues and 3 (5.55 %) in CagA -HP. The molecular interactions of CagA was also showed its efficiency for HER2 expression. Conclusion:The study concluded that CagA+ HP may induce HER2 overexpression in GC tissues.

show abstract

“…During gastroduodenoscopy, immediately after entering the stomach, 5 ml of gastric juice was aspirated through the suction channel of the endoscope and collected in a trap placed in the suction line [19]. Routine inspection of the upper gastrointestinal tract was performed, and several biopsy specimens from both the antrum and the corpus were then obtained for RUT and bacteriological culture as described below.…”

Section: Gastroduodenoscopy and Diagnosis Of H Pylori Infectionmentioning

confidence: 99%

“…For the bacterial culture test, biopsy specimens were inoculated onto agar plates (E-MR82; Eiken Chemical Co, Ltd., Tochigi, Japan) and incubated at 37 °C under microaerophilic conditions for up to 7 days [19]. Colonies were identified as H. pylori based on morphology in Gram stains, oxidase test, and RUT [19].…”

Section: Gastroduodenoscopy and Diagnosis Of H Pylori Infectionmentioning

confidence: 99%

False Sero-Negative Results for Helicobacter Pylori Infection Indicate Increased Risk of Severe Atrophic Gastritis in Japanese Patients

Ichikawa¹

2014

J Carcinog Mutagen

View full text Add to dashboard Cite

Background/objective: H. pylori infection is commonly diagnosed using an anti-H. pylori IgG antibody test. However, a proportion of results are falsely sero-negative. We investigated characteristics of patients falsely seronegative for H. pylori in relation to gastric atrophy. Methods: H. pylori infection (Hp+ or Hp-) was determined based on culture test, rapid urease test (RUT), and polymerase chain reaction (PCR) test in 280 outpatients. Anti-H. pylori antibody titers ≥ 10 U/ml were diagnosed as sero-positive for H. pylori (IgG+), while those <10 U/ml were sero-negative (IgG-). Serum pepsinogen (PG) I/PG II ratios were calculated as a serological marker of gastric atrophy. Endoscopic gastric mucosal atrophy was also assessed according to the Kimura-Takemoto classification system. Results: The mean PG I/PG II ratio in each group was as follows: Hp-/IgG-(4.99 ± 1.04, n=10), Hp+/IgG+ (2.59 ± 1.51, n=240), Hp-/IgG+ (5.65 ± 2.72, n=4) and Hp+/IgG-(3.02 ± 2.61, n=26). The mean serum PG I/PG II ratio in the Hp+/IgG-group was lower than those of Hp-/IgG-and Hp-/IgG+ groups (P=0.028 and 0.072). Incidence of severe gastric mucosal atrophy in the Hp+/IgG-group was highest of the four groups. Conclusions: Individuals falsely sero-negative for H. pylori infection is at increased risk of severe atrophic gastritis, which is well known as precancerous lesion.

show abstract

Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments

Cited by 34 publications

References 33 publications

Amount of Helicobacter pylori in gastric mucus during anti‐H. pylori treatment

Amount of Helicobacter pylori in gastric mucus during anti‐H. pylori treatment

Detection of HER2 expression and its structural alterations in gastric cancer tissues infected with cagA+H. pylori

False Sero-Negative Results for Helicobacter Pylori Infection Indicate Increased Risk of Severe Atrophic Gastritis in Japanese Patients

Contact Info

Product

Resources

About