Quantitative succinyl-proteome profiling of Chinese hickory (Carya cathayensis) during the grafting process

Abstract

BackgroundChinese hickory (Carya cathayensis) is a popular nut plant having high economic value. Grafting is applied to accelerate the transition from vegetative phase to reproductive phase. Lysine succinylation occurs frequently in the proteins associated with metabolic pathways, which may participate in the regulation of the grafting process. However, the exact regulatory mechanism underlying grafting process in Chinese hickory has not been studied at post-translational modification level.ResultsA comprehensive proteome-wide lysine succinylation profiling of Chinese hickory was explored by a newly developed method combining affinity enrichment and high-resolution LC-MS/MS. In total, 259 succinylation sites in 202 proteins were identified, representing the first comprehensive lysine succinylome in Chinese hickory. The succinylation was biased to occur in the cytosolic proteins of Chinese hickory. Moreover, four conserved succinylation motifs were identified in the succinylated peptides. Comparison of two grafting stages of Chinese hickory revealed that the differential expressed succinylated proteins were mainly involved in sugar metabolism, carbon fixation, amino acid metabolism and plant-pathogen interaction. Besides, seven heat shock proteins (HSPs) with 11 succinylation sites were also identified, all of which were observed to be up-regulated during the grafting process.ConclusionsSuccinylation of the proteins involved in amino acid biosynthesis might be required for a successful grafting. Succinylated HSPs might play a role in stress tolerance of the grafted Chinese hickory plants. Our results can be a good resource for functional validation of the succinylated proteins and a starting point for the investigation of molecular mechanisms during lysine succinylation occurring at grafting site.

Comprehensive Succinylome Profiling Reveals the Pivotal Role of Lysine Succinylation in Energy Metabolism and Quorum Sensing of Staphylococcus epidermidis

“…The average degree of succinylation sites per protein was 2.4 (1557/649). Compared with other species including E. coli 3.85 (2580/670), V. parahaemolyticus 3.0 (1931/642), A. hydrophila 3.3 (2174/666), M. tuberculosis 2.5 (1545/626), P. aeruginosa 2.5 (1520/612), T. rubrum 2.0 (569/284), A. flavus 2.8 (985/349), S. cerevisiae 2.8 (1345/474), D. rerio 3.4 (552/164), H. sapiens 2.7 (2004/738), M. musculus 2.9 (2140/750), C. cathayensis 1.3 (259/202), S. lycopersicum 1.7 (347/202), and T. aestivum 1.9 (330/173) ( Weinert et al, 2013 ; Pan et al, 2015 ; Yang et al, 2015 ; Jin and Wu, 2016 ; Xu et al, 2017 ; Zhang et al, 2017 ; Gaviard et al, 2018 ; Ren et al, 2018 ; Gao Y. et al, 2019 ; Yao et al, 2019 ; Yuan et al, 2019 ), S. epidermidis ranked the last in the bacterial group whose average degree was over 2.5 (see Figure 1B ).…”

Proteomic Profiling Reveals Differentially Expressed Proteins Associated with Amylose Accumulation During Rice Grain Filling

“…Proteomics pro ling has been approved as a powerful molecular strategy that has been widely implemented in dissecting the molecular basis of various biological processes in living organisms including plants. However, the experimental system and procedure of the employed proteomics pro ling approach greatly affect the powerful and e ciency of proteomic pro ling in dissecting the molecular mechanisms of a biological process [18][19][20][21][22][23][24][25][26]. In rice, seed development is a complex biological process that is greatly affect grain yield and quality and is governed by complex regulatory networks comprising numerous transcription factors [27].…”

Comparative Quantitative Proteomic Analysis Reveals Differentially Expressed Proteins Involved in Grain-Filling Rate of Rice at the Early Ripening Stage

“…However, the e ciency of proteomic analysis in identi cation of proteins underlying a given biological process greatly depends on the applied proteomics analysis procedure and the experimental system [28][29][30][31][32][33]. Although proteomic analyses have been widely employed to dissect the molecular basis of variable biological processes in plants, in particular stress tolerance, [29,32,[34][35][36][37][38][39]), to the best of our knowledge this is the rst comprehensive study conducted to dissect proteins involved in grain lling rate of rice. Seed development in rice is a complex biological process that directly impact potential yield and is regulated by complex regulatory networks involving several transcription factors [40].In the current study, two rice cultivars, i.e., Xiangzaoxian 24 and Xiangzaoxian 42, that possess contrasting phenotypes in grain lling rate during the early ripening period were employed in identi cation of grain lling differentially expressed proteins.…”